Ascorbic acid reduces the endotoxin‐induced lung injury in awake sheep

Abstract

Our aim was to investigate whether ascorbic acid can reduce reactive oxygen metabolite-mediated acute lung injury. The effects of intravenous administration of Escherichia coli endotoxin were studied, with and without ascorbic acid infusion, on haemodynamics, lung lymph flow, cardio-respiratory and neutrophil function in chronically instrumented sheep. Paired experiments were performed on eight sheep in which they received either endotoxin alone (0.5 micrograms kg-1 b.w.) (ET group) or in combination with an ascorbic acid infusion (1 g kg-1 b.w. bolus injection followed by 0.2 g kg-1 h-1 continuous infusion) ET + ASC group) in random order. Four of the animals also received ascorbic acid alone (ASC group). As a result, for the ET + ASC group a general and mostly significant improvement (P < 0.05) in the early hypertensive phase (0-60 min, P values) and in the late permeability phase (2-4 h, *P values) of cardiorespiratory function (mean artery pressure: P/*P = 0.283/0.049; mean pulmonary artery pressure: P/*P = 0.0001/0.0001; mean pulmonary artery wedge pressure: P/*P = 0.012/0.001; right ventricular stroke work index: P/*P = 0.02/0.0001; cardiac index: P/*P = 0.797/0.755; arterial oxygen saturation: P/*P = 0.0059/0.01; arterial-venous difference of oxygen tension: P/*P = 0.011/0.0005), oxygen consumption: P/*P = 0.013/0.035, lung lymph flow: P/*P = 0.562/0.012, lymph/plasma protein ratio: P/*P = 0.304/0.008 and protein clearance: P/*P = 0.56/0.05 was observed in comparison with the ET group.(ABSTRACT TRUNCATED AT 250 WORDS)

EPCK1, a Vitamin C and E Analogue, Reduces Endotoxin-Induced Systemic Inflammation in Mice

“…reported that vitamin C improved sepsis and sepsisrelated organ damage [11]. In addition, a recent study showed that co-administration of vitamin C and vitamin E significantly reduced sepsis-related inflammation and organ injury in the laboratory and in the clinical state [12,13].…”

Evaluation of Vitamin C for Adjuvant Sepsis Therapy

“…The lung injury in animal models of endotoxemia is also mitigated by vitamin C. For instance, parenteral administration of ascorbate [1000 mg/kg bolus injection followed by 200 mg/(kg.h) continuous infusion] decreases protein extravasation in the lungs of LPS-exposed sheep (15). Also, bolus injection of ascorbate or DHAA (200 mg/kg) decreases the concentration of protein in bronchoalveolar lavage fluid of LPS-exposed mice (17).…”

Early postoperative substitution procedure of the antioxidant ascorbic acid

“…In addition, the plasma concentration of AA, which is a major antioxidant of the plasma, is often lowered. Recently, the positive effects of AA substitution on ischemia-reperfusion damage [11,12], acute respiratory distress syndrome (ARDS) [13,14], systemic inflammatory response syndrome (SIRS) and sepsis [15] were shown. These effects are ascribed to the radical scavenger activity of AA.…”