Ascorbyl palmitate synthesis in an organic solvent system using a Celite-immobilized commercial lipase (Lipolase 100L)

Sharma, S.K.; Kriti, Kanwar; Kanwar, Shamsher S.

doi:10.1007/s13205-016-0486-7

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…HK‐2 cells (human proximal endothelial cells) were cultured in medium (Opti‐MEM, Manassa, VA, USA) with epidermal growth factor human recombinant and bovine pituitary extract. Palmitate (P0500; Sigma, St. Louis, MO, USA) was dissolved in 0.1% dimethyl sulfoxide into final stock concentration 10 mmol/L, and dimethyl sulfoxide was used as the control. In the presence or absence of SGLT2 inhibitor or small interfering ribonucleic acid (siRNA)‐mediated knockdown of SGLT2, palmitate (adjusted to 150 μmol/L) or high glucose (30 mmol/L) were incubated in media for 48 h.…”

Section: Methodsmentioning

confidence: 99%

Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high‐fat diet

Takagi

Takagaki

et al. 2018

J of Diabetes Invest

102

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Aims/IntroductionComplete mechanisms of renoprotective effects of sodium–glucose cotransporter 2 (SGLT2) inhibitors have not been elucidated yet. Mitochondrial biogenesis is regulated by membrane GTPases, such as optic atrophy factor 1 and mitofusion 2. Here, we investigated whether SGLT2 inhibition in mice fed with a high‐fat diet (HFD) improved mitochondrial morphology and restored mitochondrial biogenesis‐related molecules.Materials and MethodsMice were fed a control diet or HFD with or without ipragliflozin treatment. After 16 weeks, the kidneys were taken out and utilized for the analysis.Results HFD‐fed mice treated with ipragliflozin showed increased caloric intake and ate more food than the control HFD‐fed mice. Body and kidney weights, and blood glucose levels were not altered by ipragliflozin treatment in HFD‐fed mice. Histological analysis showed that, compared with control mice, HFD‐fed mice displayed tubular vacuolation, dilatation and epithelial cell detachment; ipragliflozin ameliorated these alterations. Furthermore, ultrastructural analysis showed that the tubule mitochondria of HFD‐fed mice exhibited significant damage. Again, ipragliflozin reversed the damage to a normal state, and restored optic atrophy factor 1 and mitofusion 2 levels in HFD‐fed mice. Increased urine 8‐hydroxydeoxyguanosine levels in HFD‐fed mice were suppressed by ipragliflozin as well. In vitro experiments using HK‐2 cells revealed that either high glucose or high palmitate suppressed optic atrophy factor 1 and mitofusion 2 levels. Suppression of SGLT2 by a specific small interfering ribonucleic acid or ipragliflozin restored these GTPase levels to their normal values.Conclusions SGLT2 inhibition might act directly on tubular cells and protect kidney tubular cells from mitochondrial damage by metabolic insults regardless of blood glucose levels or improvement in bodyweight reduction.

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Section: Methodsmentioning

confidence: 99%

Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high‐fat diet

Takagi

Takagaki

et al. 2018

J of Diabetes Invest

102

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“…Santibañez, et al [20] used the enzyme immobilized in octyl-agarose obtaining a synthesis conversion of 57% at an AA:PA molar ratio of 1:5, using tert-butanol as solvent. Sharma, et al [22] evaluated the esterification of AA with PA, using 1 M AA and 2.5 M PA in dimethyl sulfoxide (DMSO) at 75 °C for 18 h, reaching 80% of synthesis conversion using the commercial lipase Lipolase 100 L. Recently, synthesis of AsPa in tert-butyl alcohol was carried out using immobilized lipase from Candida antarctica B at optimized reaction conditions obtaining in conversion of 90% [23]. Despite several studies have been published in this topic, there are no reports about the combined effect of higher AA:PA molar ratios and temperature, neither the evaluation of the influence of more polar solvents on the enzymatic performance in AsPa production, which could lead to high synthesis yield under softer reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis with Immobilized Lipases and Downstream Processing of Ascorbyl Palmitate

et al. 2019

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Ascorbyl palmitate is a fatty acid ester endowed with antioxidant properties, used as a food additive and cosmetic ingredient, which is presently produced by chemical synthesis. Ascorbyl palmitate was synthesized from ascorbic acid and palmitic acid with a Pseudomonas stutzeri lipase immobilized on octyl silica, and also with the commercial immobilized lipase Novozym 435. The latter was selected for optimizing the reaction conditions because of its high reactivity and stability in the solvent 2-methyl-2-butanol used as reaction medium. The reaction of the synthesis was studied considering temperature and molar ratio of substrates as variables and synthesis yield as response parameter. The highest yield in the synthesis of ascorbyl palmitate was 81%, obtained at 55 °C and an ascorbic acid to palmitic acid molar ratio of 1:8, both variables having a strong effect on yield. The synthesized ascorbyl palmitate was purified to 94.4%, with a purification yield of 84.2%. The use of generally recognized as safe (GRAS) certified solvents with a polarity suitable for the solubilization of the compounds made the process a viable alternative for the synthesis and downstream processing of ascorbyl palmitate.

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“…(Thirunavukarasu et al, 2008). Tween 20 also increases the production of Bacillus altitudinis AP-MSU esterases (Palanichamy et al, 2012), but inhibition of lipase activity is detected in the presence of surfactants (Tween-20, Tween-80 and Triton X-100) tested on Celite-immobilized commercial lipase (Lipolase 100 L) (Sharma et al, 2016). It was also noted that lipases produced by Pseudomonas aeruginosa HFE733 are activated by beta-mercaptoethanol and cysteine (Jun et al, 2018).…”

Section: Effect Of Metal Ions and Additives On Lipase Activitymentioning

confidence: 99%

Lipase and esterase activities of lactic acid bacteria isolated from different biotopes

Dellali¹,

Karam²,

Karam³

2020

Afr. J. Biotechnol.

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The lipolytic and esterase activities of fifteen strains of lactic acid bacteria isolated from different biotopes of Algeria and Mauritania were tested on MRS medium supplemented with lipidic substrates. Five of them showed maximum activity in the presence of tributyrin; the activity is therefore a tributyrin esterase. These strains were identified by MALDI-TOF in Enterococcus faecium and Enterococcus durans. The study of growth kinetics as a function of time shows a start of fatty acid production during the exponential phase to reach its maximum in the stationary phase. A better esterase activity is observed at between pH 6 and 9 and at an optimal temperature of 30 to 40°C for the five strains. The influence of metal and additive ions on the esterase activity varies between bacteria but generally, total inhibition was observed in all strains tested in the presence of SDS, NaN 3 , CuCl 2 , EDTA, AgNO 3 and HgCl 2 .

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Ascorbyl palmitate synthesis in an organic solvent system using a Celite-immobilized commercial lipase (Lipolase 100L)

Cited by 11 publications

References 26 publications

Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high‐fat diet

Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high‐fat diet

Synthesis with Immobilized Lipases and Downstream Processing of Ascorbyl Palmitate

Lipase and esterase activities of lactic acid bacteria isolated from different biotopes

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