Asexual reproductive organ-specific expression of the glyceraldehyde-3-phosphate dehydrogenase 2 gene of Pilobolus crystallinus

Abstract

Pilobolus crystallinus has three putative glyceraldehyde-3-phosphate dehydrogenase (gapdh) genes (pcgapdh1, pcgapdh2 and pcgapdh3). The results of this study demonstrate that expression of pcgapdh2 was increased by irradiation and that this increased expression was correlated with the formation of asexual reproductive organs (trophocysts). Interestingly, expression of pcgapdh2 was restricted to trophocysts. The formation of trophocysts was likely promoted by light, and the expression of pcgapdh2 was increased as a result of trophocyst formation. This is the first report that shows the regulation of a gapdh gene in an organ-specific manner in fungi.

Cloning of glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycete mushroom Pleurotus ostreatus and analysis of their expression during fruit-body development

“…In L. edodes, the GPD gene is expressed constitutively during each stage of fruit-body development and in the two parts of the mature fruit body, the gills and cap (Hirano et al 1999). However, GPD gene expression has been reported to be regulated under specific metabolic and environmental conditions in S. cerevisiae (McAlister and Holland 1985;Boucherié et al 1995), L. sajor-caju (Jeong et al 2000), and R. toruloides (Liu et al 2013), and during cell growth and differentiation in T. harzianum (Puyesky et al 1997), P. brasiliensis (Barbosa et al 2004), and P. crystallinus (Kubo 2012), although the reason for regulation is not fully understood. Accordingly, our results suggest that the expression of PoGPD1 and PoGPD2 is developmentally regulated in P. ostreatus strain H1, PoGPD1 and PoGPD2 proteins may serve to supply energy and substrates through glycolysis and gluconeogenesis preferentially in the mycelial and primordial stages and in the base as compared to other parts of mature fruit bodies.…”

“…During cellular growth and differentiation, GPD gene expression is induced during the transition from mycelium to yeast in Paracoccidioides brasiliensis (Barbosa et al 2004), while GPD gene expression is repressed during conidiation and mycoparasitism in Trichoderma harzianum (Puyesky et al 1997). In addition, the GPD gene is specifically expressed in asexual reproductive organs of Pilobolus crystallinus (Kubo 2012). These findings raise the question of whether GPD gene expression is regulated during the developmental process in basidiomycetous fruit bodies.…”