Asexual Sporulation in
            <i>Aspergillus nidulans</i>

Adams, Tom; Wieser, Jenny; Yu, Jae‐Hyuk

doi:10.1128/mmbr.62.2.545-545.1998

Cited by 50 publications

(87 citation statements)

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“…In contrast, A. nidulans DmedA mutants exhibit a medusoid phenotype resulting from a proliferation of branching chains of primary sterigmata (metulae), in addition to delayed phialide and conidia production (Clutterbuck, 1969;Aguirre, 1993). One possible explanation for this observation is that A. fumigatus produces uniseriate conidiophores that lack metulae, while those of A. nidulans are biseriate (Adams et al, 1998, Latge, 1999. These observations suggest that MedA exerts specific effects on the metulae of A. nidulans to produce the medusoid phenotype, and the lack of this cell type in A. fumigatus results in a simple delay of phialide and conidia production.…”

Section: Discussionmentioning

confidence: 99%

Aspergillus fumigatusMedA governs adherence, host cell interactions and virulence

Gravelat

Ejzykowicz

Chiang

et al. 2010

Cellular Microbiology

126

148

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SummaryIn medically important fungi, regulatory elements that control development and asexual reproduction often govern the expression of virulence traits. We therefore cloned the Aspergillus fumigatus developmental modifier MedA and characterized its role in conidiation, host cell interactions and virulence. As in the model organism Aspergillus nidulans, disruption of medA in A. fumigatus dramatically reduced conidiation. However, the conidiophore morphology was markedly different between the two species. Further, gene expression analysis suggested that MedA governs conidiation through different pathways in A. fumigatus compared with A. nidulans. The A. fumigatus DmedA strain was impaired in biofilm production and adherence to plastic, as well as adherence to pulmonary epithelial cells, endothelial cells and fibronectin in vitro. The DmedA strain also had reduced capacity to damage pulmonary epithelial cells, and stimulate pro-inflammatory cytokine mRNA and protein expression. Consistent with these results, the A. fumigatus DmedA strain also exhibited reduced virulence in both an invertebrate and a mammalian model of invasive aspergillosis. Collectively, these results suggest that the downstream targets of A. fumigatus MedA mediate virulence, and may provide novel therapeutic targets for invasive aspergillosis.

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Section: Discussionmentioning

confidence: 99%

Aspergillus fumigatusMedA governs adherence, host cell interactions and virulence

Gravelat

Ejzykowicz

Chiang

et al. 2010

Cellular Microbiology

126

148

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“…The wA functionality in pigment formation was confirmed by gene-deletion studies [ 19 ]. The lack of clustering of the two A. nidulans conidial pigment genes also became evident by their different expression patterns and the finding that they are controlled by different regulatory systems [ 20 , 21 ]. The yA gene is expressed in phialide cells and primary sterigmata (metulae) [ 18 ], and controlled by BrlA and AbaA [ 21 ], while wA is expressed only in phialides [ 22 ] and controlled by WetA [ 20 ].…”

Section: Naphthopyronementioning

confidence: 99%

Genetics of Polyketide Metabolism in Aspergillus nidulans

et al. 2012

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Secondary metabolites are small molecules that show large structural diversity and a broad range of bioactivities. Some metabolites are attractive as drugs or pigments while others act as harmful mycotoxins. Filamentous fungi have the capacity to produce a wide array of secondary metabolites including polyketides. The majority of genes required for production of these metabolites are mostly organized in gene clusters, which often are silent or barely expressed under laboratory conditions, making discovery and analysis difficult. Fortunately, the genome sequences of several filamentous fungi are publicly available, greatly facilitating the establishment of links between genes and metabolites. This review covers the attempts being made to trigger the activation of polyketide metabolism in the fungal model organism Aspergillus nidulans. Moreover, it will provide an overview of the pathways where ten polyketide synthase genes have been coupled to polyketide products. Therefore, the proposed biosynthesis of the following metabolites will be presented; naphthopyrone, sterigmatocystin, aspyridones, emericellamides, asperthecin, asperfuranone, monodictyphenone/emodin, orsellinic acid, and the austinols.

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“…This is due to the that, for the mold growth cycles, the mold grows fast and turns to mature in the first 36 h; then the mycelium growth gradually decreases. Consequently, the rate of mold growth reduced after 36 h of cultivation [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Study on the Anti-Biodegradation Property of Tunicate Cellulose

Chen

Mondal

et al. 2020

Polymers

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Tunicate is a kind of marine animal, and its outer sheath consists of almost pure Iβ crystalline cellulose. Due to its high aspect ratio, tunicate cellulose has excellent physical properties. It draws extensive attention in the construction of robust functional materials. However, there is little research on its biological activity. In this study, cellulose enzymatic hydrolysis was conducted on tunicate cellulose. During the hydrolysis, the crystalline behaviors, i.e., crystallinity index (CrI), crystalline size and degree of polymerization (DP), were analyzed on the tunicate cellulose. As comparisons, similar hydrolyses were performed on cellulose samples with relatively low CrI, namely α-cellulose and amorphous cellulose. The results showed that the CrI of tunicate cellulose and α-cellulose was 93.9% and 70.9%, respectively; and after 96 h of hydrolysis, the crystallinity, crystalline size and DP remained constant on the tunicate cellulose, and the cellulose conversion rate was below 7.8%. While the crystalline structure of α-cellulose was significantly damaged and the cellulose conversion rate exceeded 83.8% at the end of 72 h hydrolysis, the amorphous cellulose was completely converted to glucose after 7 h hydrolysis, and the DP decreased about 27.9%. In addition, tunicate cellulose has high anti-mold abilities, owing to its highly crystalized Iβ lattice. It can be concluded that tunicate cellulose has significant resistance to enzymatic hydrolysis and could be potentially applied as anti-biodegradation materials.

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Asexual Sporulation in Aspergillus nidulans

Cited by 50 publications

References 0 publications

Aspergillus fumigatusMedA governs adherence, host cell interactions and virulence

Aspergillus fumigatusMedA governs adherence, host cell interactions and virulence

Genetics of Polyketide Metabolism in Aspergillus nidulans

Study on the Anti-Biodegradation Property of Tunicate Cellulose

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