Asian-common strains of ‘Candidatus Liberibacter asiaticus’ are distributed in Northeast India, Papua New Guinea and Timor-Leste

Miyata, Sumiko; Katô, Hiroshi; Davis, R. M.; Smith, Malcolm W.; Weinert, M.; Iwanami, Toru

doi:10.1007/s10327-010-0284-8

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…The presence of HLB in the North-Eastern region of India (Sikkim, West Bengal, Meghalaya, Assam) has been confirmed by PCR analyses, which identified Las not only in suspicious plant samples but also in psyllids from affected orchards in the regions visited (Das et al, 2007;Miyata et al, 2011). Las has also been reported from Vidarbha in Maharashtra (Das, 2009).…”

Section: Indian Subcontinentmentioning

confidence: 88%

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Keynote Address: Heat-tolerant Asian HLB meets heat-sensitive African HLB in the Arabian Peninsula! Why?

Bové¹

2014

Journal of Citrus Pathology

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Section: Indian Subcontinentmentioning

confidence: 88%

“…None of the sixteen samples collected in five locations from the eastern part of the country was positive, and the psyllid vector was not detected in these locations. Las was again identified in three leaf samples from East Timor by Miyata et al (2011).…”

Section: Timor Islandmentioning

confidence: 96%

Keynote Address: Heat-tolerant Asian HLB meets heat-sensitive African HLB in the Arabian Peninsula! Why?

Bové¹

2014

Journal of Citrus Pathology

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“…In addition to these genes, the rplKAJL-rpoBC operon sequence, nusG-rplK , and bacteriophage-type DNA polymerase region [30], [31], [32], [33] have been used for genetic differentiation of C Las. The completion of the C Las genome sequence [34] has allowed identifying different regions of the genome with variable number of tandem repeats (VNTRs) [35], [36], [37], [38], [39].…”

Section: Introductionmentioning

confidence: 99%

Validation of ‘Variable Number of Tandem Repeat’-Based Approach for Examination of ‘Candidatus Liberibacter asiaticus’ Diversity and Its Applications for the Analysis of the Pathogen Populations in the Areas of Recent Introduction

et al. 2013

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Citrus greening (Huanglongbing, HLB) is one of the most destructive diseases of citrus worldwide. In South Asia HLB has been known for more than a century, while in Americas the disease was found relatively recently. HLB is associated with three species of ‘Candidatus Liberibacter’ among which ‘Ca. Liberibacter asiaticus’ (CLas) has most wide distribution. Recently, a number of studies identified different regions in the CLas genome with variable number of tandem repeats (VNTRs) that could be used for examination of CLas diversity. One of the objectives of the work presented here was to further validate the VNTR analysis-based approach by assessing the stability of these repeats upon multiplication of the pathogen in a host over an extended period of time and upon its passaging from a host to a host using CLas populations from Florida. Our results showed that the numbers of tandem repeats in the four loci tested display very distinguishable “signature profiles” for the two Florida-type CLas haplotype groups. Remarkably, the profiles do not change upon passage of the pathogen in citrus and psyllid hosts as well as after its presence within a host over a period of five years, suggesting that VNTR analysis-based approach represents a valid methodology for examination of the pathogen populations in various geographical regions. Interestingly, an extended analysis of CLas populations in different locations throughout Florida and in several countries in the Caribbean and Central America regions and in Mexico where the pathogen has been introduced recently demonstrated the dispersion of the same haplotypes of CLas. On the other hand, these CLas populations appeared to differ significantly from those obtained from locations where the disease has been present for a much longer time.

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“…A few single nucleotide changes have been identified in the sequences of 16S rDNA, the omp gene, the wserAtrmU-tufB-secE-nusG-rplKAJL-rpoB gene cluster, and the flanking regions in various Las isolates (Adkar- Purushothama et al 2009;Furuya et al 2010;Miyata et al 2011). Due to their many mutations, this upstream region and the DNA pol region are not suitable for detection and diagnosis using PCR or LAMP methods.…”

mentioning

confidence: 98%

Recombination-like sequences in the upstream region of the phage-type DNA polymerase in ‘Candidatus Liberibacter asiaticus’

Furuya

Truc²,

Iwanami

2011

J Gen Plant Pathol

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We determined and compared nucleotide sequences in the upstream region of the bacteriophage-type DNA polymerase in 15 Asian isolates of the citrus-greening bacterium 'Candidatus Liberibacter asiaticus' using the genomic information of American isolate Psy62. Some isolates had nucleotide changes and a large 156-bp deletion, which were concentrated in the putative bacteriophage genes ORF3 and ORF4 and corresponding intergenic region. Eight partial recombination-like patterns were observed in this variable region. Most were confirmed to have occurred between the original types 1 and 2, but a fragment, only several dozen nucleotides long and possibly derived from a third type, was also identified.

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Asian-common strains of ‘Candidatus Liberibacter asiaticus’ are distributed in Northeast India, Papua New Guinea and Timor-Leste

Cited by 10 publications

References 12 publications

Keynote Address: Heat-tolerant Asian HLB meets heat-sensitive African HLB in the Arabian Peninsula! Why?

Keynote Address: Heat-tolerant Asian HLB meets heat-sensitive African HLB in the Arabian Peninsula! Why?

Validation of ‘Variable Number of Tandem Repeat’-Based Approach for Examination of ‘Candidatus Liberibacter asiaticus’ Diversity and Its Applications for the Analysis of the Pathogen Populations in the Areas of Recent Introduction

Recombination-like sequences in the upstream region of the phage-type DNA polymerase in ‘Candidatus Liberibacter asiaticus’

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