Asiaticoside enhances normal human skin cell migration, attachment and growth in vitro wound healing model

The potential of asiaticoside for TGF-β1/Smad signaling inhibition in prevention and progression of hypoxia-induced pulmonary hypertension

“…The direct inhibition by asiaticoside of PASMCs might result in the suppression of vascular remodeling. Yet, it was also reported [19,20] that asiaticoside enhanced normal human skin cell growth, migration and attachment in vitro, and induced type I collagen synthesis via the activation of the TGF-beta receptor I kinase (TbetaRI kinase)-independent Smad pathway, presenting a different side of asiaticoside. Diverse results might be explained by different cell types, experiment condition and duration of drug treatment.…”

Chitosan–aluminum monostearate composite sponge dressing containing asiaticoside for wound healing and angiogenesis promotion in chronic wound

“…Asiaticoside at some concentrations significantly promoted the NHDF proliferation. Stimulating effect on fibroblast proliferation of asiaticoside has been reported from both in vitro and in vivo studies [47][48][49]. Mechanism at molecular and gene expression levels of asiaticoside on fibroblast activities was studied previously [50].…”

Shikonin promotes intestinal wound healing in vitro via induction of TGF-β release in IEC-18 cells

“…Cells were subsequently cultured in fresh serum-deprived medium (0.5% foetal bovine serum) supplemented with either TGF-b 1 Sigma-Aldrich) (5 ng/mL), anti-TGF-b 1 (R&D Systems, Minneapolis, MN, USA) (10 lg/mL), shikonin 1 lM (TCI Europe, Zwijndrecht, Belgium), shikonin 1 lM + anti-TGF-b 1 (10 lg/mL), asiaticoside 15.6 lM (Sigma-Aldrich), dexamethasone 10 lM (Sigma-Aldrich) or left untreated (blank). We use asiaticoside as a positive standard because it has a long history of use for treating skin and vascular diseases in Asia, and has recently been demonstrated to increase wound healing in vitro through an increase in cell migration (Lee et al, 2012). The migration of the cells across the wound line was assessed 24 h later in a blind fashion with the aid of ImageJ software for Mac OS X (US National Institutes of Health) (Bu et al, 2007).…”