Asiaticoside exerts neuroprotection through targeting NLRP3 inflammasome activation

He, Ziliang; Hu, Yeye; Zhang, Ying; Xie, Jing; Niu, Zhiqiang; Yang, Guigui; Zhang, Ji; Zhao, Zixuan; Wei, Shuai; Wu, Haifeng; Hu, Weicheng

doi:10.1016/j.phymed.2024.155494

Cited by 7 publications

(2 citation statements)

References 31 publications

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“…The protein structure was optimized and refined using the Fast Preparation module . All water molecules were removed . In the dock option, the pretreated proteins were selected as receptors and CA as ligands.…”

Section: Methodsmentioning

confidence: 99%

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Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Zhang,

Mu,

Wang

et al. 2024

J. Proteome Res.

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Dental caries is a chronic oral infectious disease, and Streptococcus mutans (S. mutans) plays an important role in the formation of dental caries. Trans-cinnamaldehyde (CA) exhibits broad-spectrum antibacterial activity; however, its target and mechanism of action of CA on S. mutans needs to be further explored. In this study, it was verified that CA could inhibit the growth and biofilm formation of S. mutans. Further proteomic analysis identified 33, 55, and 78 differentially expressed proteins (DEPs) in S. mutans treated with CA for 1, 2, and 4 h, respectively. Bioinformatics analysis showed that CA interfered with carbohydrate metabolism, glycolysis, pyruvate metabolism, and the TCA cycle, as well as amino acid metabolism of S. mutans. Protein interactions suggested that pyruvate dehydrogenase (PDH) plays an important role in the antibacterial effect of CA. Moreover, the upstream and downstream pathways related to PDH were verified by various assays, and the results proved that CA not only suppressed the glucose and sucrose consumption and inhibited glucosyltransferase (GTF) and lactate dehydrogenase (LDH) activities but also decreased the ATP production. Interestingly, the protein interaction, qRT-PCR, and molecular docking analysis showed that PDH might be the target of CA to fight S. mutans. In summary, the study shows that CA interferes with the carbohydrate metabolism of bacteria by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle via binding to PDH, which verifies that PDH is a potential target for the development of new drugs against S. mutans.

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Section: Methodsmentioning

confidence: 99%

“…37 All water molecules were removed. 38 In the dock option, the pretreated proteins were selected as receptors and CA as ligands.…”

Section: Molecular Dockingmentioning

confidence: 99%

Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Zhang,

Mu,

Wang

et al. 2024

J. Proteome Res.

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Asiaticoside enhances the effect of propofol on the invasion, ferroptosis and immune escape of bladder cancer

Jin,

He,

Zhen

et al. 2024

Drug Development Research

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Bladder cancer is a highly prevalent malignancy. Asiaticoside (AC), a triterpenoid derivative, exhibits antitumor effect on different tumors. This study aimed to explore the role and mechanism of AC on bladder cancer. J82 and T24 cells were treated with AC and/or propofol, and nude mice were subcutaneously administrated with T24 cells. The effect and mechanism of AC and/or propofol were explored by cell counting kit‐8, transwell, flow cytometry, enzyme‐linked immunosorbent assay, immunohistochemistry and western blot assays both in vitro and in vivo. Cell viability of J82 and T24 cells was inhibited by AC with a IC50 value of 2.43 μM and 2.16 μM, and by propofol with a IC50 value of 42.51 μM and 48.37 μM, respectively. AC or propofol alone decreased cell proliferation, invasion, and immune escape with the increased ferroptosis, as well as downregulating the level of the PI3K/AKT pathway in both animal and cell experiments. The effect of propofol on the above‐mentioned indicators was further enhanced with the co‐treatment of AC in vitro and in vivo. Taken together, AC promoted the ameliorative effect of propofol on bladder cancer involved in PI3K/AKT pathway.

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A new frontier in neuropharmacology: Recent progress in natural products research for blood–brain barrier crossing

Isabel,

de la Riera M. Belén,

Dolores R

et al. 2024

Current Research in Biotechnology

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Asiaticoside exerts neuroprotection through targeting NLRP3 inflammasome activation

Cited by 7 publications

References 31 publications

Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Asiaticoside enhances the effect of propofol on the invasion, ferroptosis and immune escape of bladder cancer

A new frontier in neuropharmacology: Recent progress in natural products research for blood–brain barrier crossing

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