Asociación entre obesidad abdominal y daño oxidativo seminal en pacientes con síndrome metabólico

Rosety, Ignacio; Elosegui, S.; Pery, María Teresa; Fornieles, Gabriel; Rosety, J.M.; Díaz, Antonio; Rodríguez-Pareja, A.; Brenes, Francisco; Rosety-Rodríguez, Manuel; Ordóñez, Francisco Javier; Rosety, Miguel Angel

doi:10.4067/s0034-98872014000600007

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Sperm defects are a result of a defective interaction between the acrosome-manchette complex and membrane microdomains. Thus, on the basis of above results and evidence in humans [ 50 , 51 , 52 , 53 ], it can be proposed that hypercholesterolemia is an important factor contributing to male infertility. Therefore, the correlation between semen parameters and serum cholesterol level may help in diagnosis and treatment of male infertility, especially in idiopathic cases.…”

Section: Discussionmentioning

confidence: 98%

Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model

et al. 2017

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Hypercholesterolemia is a marker for several adult chronic diseases. Recently we demonstrated that sub/infertility is also associated to Hypercholesterolemia in rabbits. Seminal alterations included: abnormal sperm morphology, decreased sperm number and declined percentage of motile sperm, among others. In this work, our objective was to evaluate the effects of hypercholesterolemia on testicular efficiency and spermiogenesis, as the latter are directly related to sperm number and morphology respectively. Tubular efficiency was determined by comparing total number of spermatogenic cells with each cell type within the proliferation/differentiation compartments. We found lower testicular efficiency related to both a decrease in spermatogonial cells and an increase in germ cell apoptosis in hypercholesterolemic rabbits. On the other hand, spermiogenesis–the last step of spermatogenesis involved in sperm shaping–was detaily analyzed, particularly the acrosome-nucleus-manchette complex. The manchette is a microtubular-based temporary structure responsible in sperm cell elongation. We analyzed the contribution of actin filaments and raft microdomains in the arrangement of the manchette. Under fluorescence microscopy, spermatocyte to sperm cell development was followed in cells isolated from V to VIII tubular stages. In cells from hypercholesterolemic rabbits, abnormal development of acrosome, nucleus and inaccurate tail implantation were associated with actin–alpha-tubulin–GM1 sphingolipid altered distribution. Morphological alterations were also observed at electron microscopy. We demonstrated for the first time that GM1-enriched microdomains together with actin filaments and microtubules are involved in allowing the correct anchoring of the manchette complex. In conclusion, cholesterol enriched diets promote male fertility alterations by affecting critical steps in sperm development: spermatogenesis and spermiogenesis. It was also demonstrated that hypercholesterolemic rabbit model is a useful tool to study serum cholesterol increment linked to sub/infertility.

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Section: Discussionmentioning

confidence: 98%

Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model

et al. 2017

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“…Although not all [3, 6, 39], several other authors found alterations in sperm quality secondary to MetS or MetS-related diets [4, 7, 8, 9, 10, 23, 40]. The sperm variables most affected are sperm concentration and motility.…”

Section: Discussionmentioning

confidence: 99%

“…In humans, MetS has been associated with hypogonadism [1, 3, 4]; in fact, some authors suggested that this trait should be added to the group of abnormalities that characterizes MetS [5]. Moreover, although without consensus [3, 6], some studies have reported a decrease in human seminal quality secondary to this pathology [4, 7, 8, 9, 10]. Specifically, some studies proposed that the oxidative stress associated to MetS and related diseases (such as obesity and type 2-diabetes) results in sperm membrane lipid peroxidation, with the consequent membrane dysfunction, motility decrease and DNA damage [reviewed in 1, 7].…”

Section: Introductionmentioning

confidence: 99%

“…However, despite the essential role of epididymis in sperm function and fertilizing capability, the possible deleterious effects of MetS (or related pathologies) on this tissue have rarely been studied. Moreover, since epididymal maturation requires a fine tuned balance between reactive oxygen species (ROS) production and neutralization, systemic or local alterations in the redox state may negatively impact semen quality [7, 26]. It is important to remark that several sperm parameters may be altered by epididymal dysfunction, including motility, sperm count and even morphology [23, 27, 28].…”

Section: Introductionmentioning

confidence: 99%

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Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet

Ramı́rez

Luque

Jones

et al. 2019

Heliyon

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The objectives of this study were: 1) to evaluate the effects of a fructose enriched diet (FED) on rat sperm quality, epididymal function (i.e. oxidative stress and alpha-glucosidase expression) and testosterone concentrations; 2) to determine if the administration of ghrelin (Ghrl), reverses the effects induced by FED. After validating the protocol as an inductor of metabolic syndrome like-symptoms, adult male rats were assigned to one of the following treatments for 8 weeks: FED ¼ 10% fructose enriched in water (v/v); FED þ Ghrl ¼ fructose enriched diet plus Ghrl (6 nmol/animal/day, s.c.) from week 6-8; or C ¼ water without fructose (n ¼ 5-10 animals/group). FED significantly decreased sperm concentration and motile sperm count/ml vs C (FED: 19.0 AE 1.6 Â 10 6 sperm/ml and 834.6 AE 137.0, respectively vs C: 25.8 AE 2.8 Â 10 6 and 1300.4 AE 202.4, respectively; p < 0.05); ghrelin injection reversed this negative effect (23.5 AE 1.6 Â 10 6 sperm/ml and 1381.7 AE 71.3 respectively). FED resulted in hypogonadism, but Ghrl could not normalize testosterone concentrations (C: 1.4 AE 0.1 ng/ml vs FED: 0.8 AE 0.2 ng/ml and FED þ Ghrl: 0.6 AE 0.2 ng/ml; p < 0.05). Ghrelin did not reverse metabolic abnormalities secondary to FED. FED did not alter epididymal expression of antioxidants enzymes (superoxido-dismutase, catalase and glutathione peroxidases-Gpx-). Nevertheless, FED þ Ghrl significantly increased the expression of Gpx3 (FED þ Ghrl: 3.47 AE 0.48 vs FED: 0.69 AE 0.28 and C: 1.00 AE 0.14; p < 0.05). The expression of neutral alpha-glucosidase, which is a marker of epididymal function, did not differ between treatments. In conclusion, the administration of Ghrl modulated the negative effects of FED on sperm quality, possibly by an epididymal increase in Gpx3 expression. However, Ghrl could not neither normalize the metabolism of FED animals, nor reverse hypogonadism.

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“…Nowadays, the incidence of conditions related to metabolic syndrome is rapidly rising worldwide, including oxidative damage, high blood sugar, hypertension, hyperlipidaemic diseases, and so on [1,2]. Nutritionists have confirmed that oxidative stress and high-calorie diets were closely related to the occurrence of chronic metabolic syndrome [3].…”

Section: Introductionmentioning

confidence: 99%

Free and Bound Phenolics of Buckwheat Varieties: HPLC Characterization, Antioxidant Activity, and Inhibitory Potency towards α-Glucosidase with Molecular Docking Analysis

et al. 2019

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Free and bound phenolic fractions from six buckwheat varieties were investigated for their compositions, antioxidant activities, and inhibitory effects on α-glucosidase. The results showed that different buckwheat varieties have significant differences in phenolic/flavonoid contents, and these contents were found in higher quantities in free form than in bound form. HPLC results revealed that rutin, quercetin, and kaempferol-3-O-rutinoside were the most abundant components in free and bound forms, whereas dihydromyricetin was found only in the bound form. Free phenolics showed higher antioxidant activities of DPPH, ABTS+, OH•, and FRAP than those of bound phenolics. Strong inhibitory effects against α-glucosidase by the free/bound phenolic fractions were found in all buckwheat varieties, and free phenolics showed stronger α-glucosidase inhibition than that of the corresponding bound phenolics. More importantly, the main phenolic compounds observed in the buckwheat varieties were subjected to molecular docking analysis to provide insight into their interactions with α-glucosidase. The contributions by individual phenolics to the observed variation was analysed by Pearson correlation coefficient analysis and principal component analysis. The present study provides a comprehensive comparison for the phenolic fractions of buckwheat varieties and identify the main contributors to antioxidant and α-glucosidase inhibitory activity.

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Asociación entre obesidad abdominal y daño oxidativo seminal en pacientes con síndrome metabólico

Abstract: (Rev Med Chile 2014; 142: 732-737)

Cited by 8 publications

References 26 publications

Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model

Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model

Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet

Free and Bound Phenolics of Buckwheat Varieties: HPLC Characterization, Antioxidant Activity, and Inhibitory Potency towards α-Glucosidase with Molecular Docking Analysis

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