Aspartate analysis in formulations using a new enzyme sensor

Cloning, expression, characterisation and mutational analysis of l-aspartate oxidase from Pseudomonas putida

“…As part of an investigation to find new enzymes capable of being incorporated into the chemo-enzymatic reaction cycles described above, or with potential application as part of biosensors for laspartate [16,17], the gene nadB encoding the l-aspartate oxidase from Pseudomonas putida (PpLASPO) has been cloned and expressed in E. coli. PpLASPO (Uniprot code Q88MZ2) has 63% sequence identity with the EcLASPO studied previously.…”

Quantification of aspartame in commercial sweeteners by FT-Raman spectroscopy

“…However, there are also examples of the use of capillary electrophoresis (Herrmannovà, Křivànkova, Bartoš, & Vytřas, 2005;Pesek & Matyska, 1997), flow injection (Capitán-Vallvey, Valencia, Nicolás, & García-Jiménez, 2006;Peña, Limba, & Saraiva, 2004), biosensors (Campanella, Aturki, Sammartino, & Tomassetti, 1995) and voltammetry (Medeiros, De Carvalho, Rocha-Filho, & Fatibello-Filho, 2008) applications for aspartame quantitative determination. Spectrophotometry (Cantarelli, Pellerano, Marchevsky, & Camiña, 2009) and infrared spectroscopy (Khurana, Cho, Shim, Li, & Jun, 2008;Kizil, Irudayaraj, & Seetharaman, 2002) in conjunction with multivariate techniques of data treatment were applied for aspartame quantification.…”

Flow-through Spectrophotometric Sensor for the Determination of Aspartame in Low-Calorie and Dietary Products

“…8 Different enzymatic assays have been developed for aspartame based on different enzyme arrays: for example peptidase, aminotransferase and glutamate oxidase have been used, monitoring the oxygen consumption with a Clark electrode. This method shows a detection limit of 2.5 × 10 -5 M, 9 or carboxyl esterase-alcohol oxidase with a detection limit of 5.0 × 10 -8 M. 10 Other ways explored for enzymatic sensing of aspartame include the bienzyme system carboxy peptidase and L-aspartase in an ammonia electrode, [11][12][13] or phenylalanine decarboxilase and carboxy peptidase in a carbon dioxide electrode. 14 Because most of the methods outlined above are not very selective, and because of the need to determine this sweetener in mixtures with other additives, such as preservatives and antioxidants, separating methods are most commonly used.…”