Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

Fernández, Eva; Ortíz, José E.; Pérez‐Pérez, Alejandro; Prats, Eva; Turbón, Daniel; Torres, Trinidad; Arroyo-Pardo, Eduardo

doi:10.1016/j.jas.2008.11.009

Cited by 23 publications

(13 citation statements)

References 41 publications

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“…Because the preservation of both amino acids and proteins and aDNA is highly plausible in the prehistoric remains recovered from hot, dry environments, AAR cannot inform the plausibility that the aDNA in our samples is authentic. Moreover, neither of two recent critical evaluations of AAR have found it useful as a means of assessing aDNA preservation in bones and teeth (Collins et al 2009;Fernandez et al 2009). Because cloning identifies only the majority consensus sequence, which itself can be a contaminant, it provides no confidence in the authenticity of aDNA samples unless a target sequence is unknown (e.g., as in the case of Neanderthal mtDNA sequences).…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA of Protohistoric Remains of an Arikara Population from South Dakota: Implications for the Macro-Siouan Language Hypothesis

et al. 2010

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Mitochondrial DNA (mtDNA) was extracted from skeletal remains excavated from three Arikara sites in South Dakota occupied between AD 1600 and 1832. The diagnostic markers of four mtDNA haplogroups to which most Native Americans belong (A, B, C, and D) were successfully identified in the extracts of 55 (87%) of the 63 samples studied. The frequencies of the four haplogroups were 42%, 29%, 22%, and 7%, respectively, and principal coordinates analysis and Fisher's exact tests were conducted to compare these haplogroup frequencies with those from other populations. Both analyses showed closer similarity among the Mohawk, Arikara, and Sioux populations than between any of these three and any other of the comparison populations. Portions of the first hypervariable segment (HVSI) of the mitochondrial genome were successfully amplified and sequenced for 42 of these 55 samples, and haplotype networks were constructed for two of the four haplogroups. The sharing of highly derived lineages suggests that some recent admixture of the Arikara with Algonquian-speaking and Siouan-speaking groups has occurred. The Arikara shared more ancient lineages with both Siouan and Cherokee populations than with any other population, consistent with the Macro-Siouan language hypothesis that Iroquoian, Siouan, and Caddoan languages share a relatively recent common ancestry.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA of Protohistoric Remains of an Arikara Population from South Dakota: Implications for the Macro-Siouan Language Hypothesis

et al. 2010

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“…The early development of this field in archaeology and palaeontology had a strong influence on the initial criteria that emerged regarding the precautions required for the production of verifiable data based on aDNA [16,17]. These have since been modified [18] to take account of our increased understanding of the sources of contamination [19] and the stability of Mycobacterium tuberculosis complex (MTBC) aDNA [20] as compared with other biomolecules [21,22].…”

Section: Early Biomolecular Studiesmentioning

confidence: 99%

Insights gained from palaeomicrobiology into ancient and modern tuberculosis

Donoghue

2011

Clinical Microbiology and Infection

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The direct detection of ancient Mycobacterium tuberculosis molecular biomarkers has profoundly changed our understanding of the disease in ancient and historical times. Initially, diagnosis was based on visual changes to skeletal human remains, supplemented by radiological examination. The introduction of biomolecular methods has enabled the specific identification of tuberculosis in human tissues, and has expanded our knowledge of the palaeopathological changes associated with the disease. We now realize that the incidence of past tuberculosis was greater than previously estimated, as M. tuberculosis biomarkers can be found in calcified and non-calcified tissues with non-specific or no visible pathological changes. Modern concepts of the origin and evolution of M. tuberculosis are informed by the detection of lineages of known location and date.

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“…Diagenetic alteration of protein structure may lead to cross-reactions and false identifications (Tuross et al, 1996). Aspartic (Asp) acid racemization ratios have generally been used to assess the potential for aDNA and protein preservation in an archaeological assemblage (Poinar et al, 1996; for a critique on the usage of racemization to assess aDNA preservation see Ferná ndez et al, 2009). Ratio values over 0.1 are interpreted as reflecting samples that have been subjected to diagenetic processes and likely will not produce well-preserved proteins.…”

Section: Pria Techniquementioning

confidence: 99%

Assessing the reliability of pRIA for identifying ancient proteins from archaeological contexts

Potter

Reuther

Lowenstein

et al. 2010

Journal of Archaeological Science

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Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

Cited by 23 publications

References 41 publications

Mitochondrial DNA of Protohistoric Remains of an Arikara Population from South Dakota: Implications for the Macro-Siouan Language Hypothesis

Mitochondrial DNA of Protohistoric Remains of an Arikara Population from South Dakota: Implications for the Macro-Siouan Language Hypothesis

Insights gained from palaeomicrobiology into ancient and modern tuberculosis

Assessing the reliability of pRIA for identifying ancient proteins from archaeological contexts

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