Aspartic proteases gene family in rice: Gene structure and expression, predicted protein features and phylogenetic relation

Chen, Jiongjiong; Ouyang, Yidan; Wang, Lei; Xie, Weibo; Zhang, Qifa

doi:10.1016/j.gene.2009.04.021

Cited by 62 publications

(76 citation statements)

References 59 publications

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“…We thus propose that OsAP25 and OsAP37 may activate downstream caspase-like protease(s) involed in PCD rather than directly acting on caspase-specific substrates. Moreover, even though OsAP25, OsAP37, PCS1 and UNDEAD belong to the C-group of aspartic proteases 43,44 , OsAP25 and OsAP37 can promote cell death (Fig. 6b-e) whereas UNDEAD and PCS1 have anti-PCD functions 31,32 , suggesting diversified roles of aspartic proteases in plant PCD.…”

Section: Discussionmentioning

confidence: 99%

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

et al. 2013

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Programmed cell death is essential for the development of multicellular organisms, yet pathways of plant programmed cell death and its regulation remain elusive. Here we report that ETERNAL TAPETUM 1, a basic helix-loop-helix transcription factor conserved in land plants, positively regulates programmed cell death in tapetal cells in rice anthers. eat1 exhibits delayed tapetal cell death and aborted pollen formation. ETERNAL TAPETUM 1 directly regulates the expression of OsAP25 and OsAP37, which encode aspartic proteases that induce programmed cell death in both yeast and plants. Expression and genetic analyses revealed that ETERNAL TAPETUM 1 acts downstream of TAPETUM DEGENERATION RETARDATION, another positive regulator of tapetal programmed cell death, and that ETERNAL TAPETUM 1 can also interact with the TAPETUM DEGENERATION RETARDATION protein. This study demonstrates that ETERNAL TAPETUM 1 promotes aspartic proteases triggering plant programmed cell death, and reveals a dynamic regulatory cascade in male reproductive development in rice.

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Section: Discussionmentioning

confidence: 99%

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

et al. 2013

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“…Aspartic proteases (APs) are a large family of proteolytic enzymes found in nearly all organisms. In plants, they are involved in many biological processes, including senescence, stress responses, programmed cell death, and reproduction (Chen et al, 2009;Guo et al, 2013). According to Contour-Ansel et al (2010), water stress in bean led to the gene expression regulation of an AP gene (PvAP1) both at the transcriptional and post-transcriptional levels, which is paramount in the conservation of cellular homeostasis and recovery.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in drought-tolerant sugarcane roots

et al. 2015

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ABSTRACT. Drought is one of the most frequent abiotic stresses limiting the productivity and geographical distribution of sugarcane culture. The use of drought-tolerant genotypes is one approach for overcoming the effects of water stress. We conducted a comparative study to identify gene expression profiles under water stress in tolerant sugarcane roots. Two different cultivars, 1 drought tolerant (RB867515) and 1 drought susceptible (SP86-155), were evaluated at 4 sampling time points (1, 3, 5, and 10 days) using the cDNA-amplified fragment length polymorphism technique. A total of 173 fragments were found to be differentially expressed in response to water stress in the tolerant cultivar. Seventy of these were cloned, sequenced, and categorized. Similarity analysis using Candidate genes to drought tolerance in sugarcane BLAST revealed that 64% of the fragments differentially expressed code proteins classified as no hits (23%), hypothetical (21%), or involved in stress response (20%), with others were involved in communication pathways and signal transduction, bioenergetics, secondary metabolism, and growth and development. Four genes were analyzed and validated using real-time quantitative polymerase chain reaction to determine their expression and showed consistency with the cDNA-amplified fragment length polymorphism analyses. Our results contribute insight into the molecular responses to water stress in sugarcane and possibility to the development of cultivars with improved tolerance to drought.

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“…These BLAST searches have usually been performed using one or several known amino acid sequences for proteins that belong to the selected gene family. Alternatively, HMMs have been constructed and used for the search [44,45]. Most analyses include additional manual curation for identified proteins to refine selection of genes belonging to the different families.…”

Section: Protein-coding Family Analyses: Peptidase Families and Theirmentioning

confidence: 99%

Plant protein-coding gene families: emerging bioinformatics approaches

Martínez

2011

Trends in Plant Science

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Protein-coding gene families are sets of similar genes with a shared evolutionary origin and, generally, with similar biological functions. In plants, the size and role of gene families has been only partially addressed. However, suitable bioinformatics tools are being developed to cluster the enormous number of sequences currently available in databases. Specifically, comparative genomic databases promise to become powerful tools for gene family annotation in plant clades. In this review, I evaluate the data retrieved from various gene family databases, the ease with which they can be extracted and how useful the extracted information is.

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Aspartic proteases gene family in rice: Gene structure and expression, predicted protein features and phylogenetic relation

Cited by 62 publications

References 59 publications

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

Differential gene expression in drought-tolerant sugarcane roots

Plant protein-coding gene families: emerging bioinformatics approaches

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