Aspartyl proteases identified as candidate genes of a fiber length QTL, qFLD05, that regulates fiber length in cotton (Gossypium hirsutum L.)

Zhang, Sujun; Wang, Haitao; Li, Xinghe; Tang, Liyuan; Cai, Xiao; Liu, Cunjing; Zhang, Xiangyun; Zhang, Jianhong

doi:10.1007/s00122-024-04559-3

Theor Appl Genet

2024

DOI: 10.1007/s00122-024-04559-3

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Aspartyl proteases identified as candidate genes of a fiber length QTL, qFLD05, that regulates fiber length in cotton (Gossypium hirsutum L.)

Sujun Zhang,

Haitao Wang,

Xinghe Li

et al.

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Cited by 3 publications

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Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers

Wang,

Liu,

Zhang

et al. 2024

New Phytologist

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Summary Length and strength are key parameters impacting the quality of textiles that can be produced from cotton fibers, and therefore are important considerations in cotton breeding. Through map‐based cloning and function analysis, we demonstrated that GhROPGEF5, encoding a ROP guanine nucleotide exchange factor, was the gene controlling fiber length and strength at qFSA10.1. Evolutionary analysis revealed that a base deletion in the third exon of GhROPGEF5 resulting in superior fiber length and strength was a rare mutation occurring in a tiny percentage of Upland cottons, with reduced fiber yield hindering its spread. GhROPGEF5 interacted with and activated GhROP10. Knockout or mutation of GhROPGEF5 resulted a loss of the ability to activate GhROP10. Knockout of GhROPGEF5 or GhROP10 affected the expression of many downstream genes associated with fiber elongation and secondary wall deposition, prolonged fiber elongation and delayed secondary wall deposition, producing denser fiber helices and increasing fiber length and strength. These results revealed new molecular aspects of fiber development and revealed a rare favorable allele for improving fiber quality in cotton breeding.

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Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers

Wang,

Liu,

Zhang

et al. 2024

New Phytologist

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An aldehyde dehydrogenase gene, GhALDH7B4_A06, positively regulates fiber strength in upland cotton (Gossypium hirsutum L.)

Tang,

Liu,

et al. 2024

Front. Plant Sci.

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High fiber strength (FS) premium cotton has significant market demand. Consequently, enhancing FS is a major objective in breeding quality cotton. However, there is a notable lack of known functionally applicable genes that can be targeted for breeding. To address this issue, our study used specific length–amplified fragment sequencing combined with bulk segregant analysis to study FS trait in an F2 population. Subsequently, we integrated these results with previous quantitative trait locus mapping results regarding fiber quality, which used simple sequence repeat markers in F2, F2:3, and recombinant inbred line populations. We identified a stable quantitative trait locus qFSA06 associated with FS located on chromosome A06 (90.74–90.83 Mb). Within this interval, we cloned a gene, GhALDH7B4_A06, which harbored a critical mutation site in coding sequences that is distinct in the two parents of the tested cotton line. In the paternal parent Ji228, the gene is normal and referred to as GhALDH7B4_A06O; however, there is a nonsense mutation in the maternal parent Ji567 that results in premature termination of protein translation, and this gene is designated as truncated GhALDH7B4_A06S. Validation using recombinant inbred lines and gene expression analysis revealed that this mutation site is correlated with cotton FS. Virus-induced gene silencing of GhALDH7B4 in cotton caused significant decreases in FS and fiber micronaire. Conversely, GhALDH7B4_A06O overexpression in Arabidopsis boosted cell wall component contents in the stem. The findings of our study provide a candidate gene for improving cotton fiber quality through molecular breeding.

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GhWRKY40 Interacts with an Asparaginase GhAPD6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.)

Zhang,

Cai,

Wei

et al. 2024

Genes

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Fiber quality improvement is a primary goal in cotton breeding. Identification of fiber quality-related genes and understanding the underlying molecular mechanisms are essential prerequisites. Previously, studies determined that silencing the gene GhWRKY40 resulted in longer cotton fibers; however, both the underlying mechanisms and whether this transcription factor is additionally involved in the regulation of cotton fiber strength/fineness are unknown. In the current study, we verified that GhWRKY40 influences the fiber strength, fiber fineness, and fiber surface structure by using virus-induced gene silencing (VIGS). Potential proteins that may interact with the nucleus-localized GhWRKY40 were screened in a yeast two-hybrid (Y2H) nuclear-system cDNA library constructed from fibers at 0, 10, and 25 days post-anthesis (DPA) in two near-isogenic lines differing in fiber length and strength. An aspartyl protease/asparaginase-related protein, GhAPD6, was identified and confirmed by Y2H and split-luciferase complementation assays. The expression of GhAPD6 was approximately 30-fold higher in the GhWRKY40-VIGS lines at 10 DPA and aspartyl protease activity was significantly upregulated in the GhWRKY40-VIGS lines at 10–20 DPA. This study suggested that GhWRKY40 may interact with GhAPD6 to regulate fiber development in cotton. The results provide a theoretical reference for the selection and breeding of high-quality cotton fibers assisted by molecular technology.

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Aspartyl proteases identified as candidate genes of a fiber length QTL, qFLD05, that regulates fiber length in cotton (Gossypium hirsutum L.)

Cited by 3 publications

References 46 publications

Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers

Natural variation in GhROPGEF5 contributes to longer and stronger cotton fibers

An aldehyde dehydrogenase gene, GhALDH7B4_A06, positively regulates fiber strength in upland cotton (Gossypium hirsutum L.)

GhWRKY40 Interacts with an Asparaginase GhAPD6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.)

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