Aspectos esenciales sobre las técnicas de fertilización in vitro en bovinos

Salgado-Cruz, Erika; Vasquez, Ricaurte Lopera

doi:10.15381/rivep.v31i3.17138

Cited by 4 publications

(1 citation statement)

References 34 publications

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“…Post-thawing, sperm motility was evaluated. "Swim up" was performed in Eppendorf tubes (Sorenson Biosciences, Inc., Salt Lake City, UT, USA) [2] in the IVM medium recovered from the oocytes that were matured from the control group [14]. Ten µL were taken from the upper part of the tubes with the motile sperm fraction (0.5 × 106 mL −1 sperm) and added to the IVF medium containing the oocytes from each treatment, incubating for 24 h under the same conditions [2].…”

Section: In Vitro Fertilization (Ivf) and In Vitro Development (Ivd) ...mentioning

confidence: 99%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

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The in vitro production and cryopreservation of mammalian embryos generates reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems to improve blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effects of resveratrol on the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0 µM, 0.25 µM, 0.5 µM, and 1 µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, were then vitrified for 24 h, and, after heating, they were stained with DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increased GSH and decreased ROS production (p < 0.05) in a dose-dependent manner. It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification by decreasing reactive oxygen species levels and facilitating the generation of embryo antioxidant enzymes like glutathione.

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Section: In Vitro Fertilization (Ivf) and In Vitro Development (Ivd) ...mentioning

confidence: 99%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

View full text Add to dashboard Cite

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Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Preprint

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The in vitro production and cryopreservation of mammalian embryos generate reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems improving blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effect of resveratrol in the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0µM, 0.25µM, 0.5µM and 1µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, then were vitrified for 24 h and after heating they were stained with DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increases GSH and decreases ROS production (p<0.05). It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification, protecting them from oxidative damage by promoting the synthesis of antioxidants.

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Ovum Pick Up (OPU) and in vitro embryo production in pregnant cows (Bos Indicus) at the Bachigualatito rancho, la Trinitaria, Chiapas

Noguez-Estrada,

Cornejo-Cervantes,

Vargas-Monter

et al. 2023

JANRE

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With the objective of determining the production, quality and development of bovine embryos (Bos Indicus), oocytes were collected from 14 females distributed in two groups: empty females (T1; n=7) and an experimental group of pregnant females (T2; n=7). The two groups were subjected to Ovum Pick Up (OPU) using disposable 18-gauge needles and a vacuum pressure of 100 mmHg. to later carry out in vitro fertilization (IVF). The study variables were the number of oocyte aspirated and fertilized and the expected embryos. A Wilcoxon test was performed for non-parametric data between two groups, finding no significant differences between treatments P> 0.05. 250 oocytes were recovered by fertilizing out of 190, obtaining a fertilization rate of 77%, reaching a transferrable blastocyst rate of 24% (45/190). In empty females (7), 113 oocytes were obtained, reaching a fertilization rate of 75% (81/113) and 25% transferable blastocysts (18/81). In the 7 pregnant females, 137 oocytes were collected, reaching a fertilization rate of 83% (109/137) and 23% transferable blastocysts (27/109). Follicular aspiration for in vitro fertilization of embryos from pregnant and empty cows is viable without differences between them, but being more variable in pregnant cows.

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Aspectos esenciales sobre las técnicas de fertilización in vitro en bovinos

Cited by 4 publications

References 34 publications

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Ovum Pick Up (OPU) and in vitro embryo production in pregnant cows (Bos Indicus) at the Bachigualatito rancho, la Trinitaria, Chiapas

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