Aspectos morfológicos da tuba uterina de cutias (Dasyprocta aguti, Mammalia: Rodentia)

Fortes, Eunice Anita de Moura; Carvalho, Maria Acelina Martins de; Almeida, Mônica Marcos de; Júnior, Aírton Mendes Conde; Cruz, Nádia Expedita de Almeida e; Neto, Antônio Chaves de Assis

doi:10.11606/issn.1678-4456.bjvras.2005.26443

Cited by 8 publications

(9 citation statements)

References 4 publications

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“…Uterine tubes in D. leporina were convoluted and divided into three regions: infundibulum, ampulla, and isthmus, which were histologically differentiated regarding the composition or thickness of serosa, mucosa, and middle layers, similar to that described for C. lanigera (Weir, ), Myocastor coypus (Felipe, Callejas, & Cabodevila, ), Hydrochoerus hydrochaeris (Costa, Paula, Fonseca, & Neves, ), Atherurus africanus (Mayor et al, ), Dasyprocta aguti (Fortes et al, ), C. paca (Reis, Gerbasi, et al, ), G. spixii (Santos et al, ), and other mammals (Favoretto, Silva, Menezes, Guerra, & Campos, ; Monteiro et al, ).…”

Section: Discussionmentioning

confidence: 87%

Morphology of the genital organs of the female red‐rumped agouti (Dasyprocta leporina, Linnaeus, 1758) during estrous cycle phases and in advanced pregnancy

Oliveira

Júnior

Sousa³

et al. 2019

Journal of Morphology

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The study investigated the gross and microscopic anatomy of the genital organs of 20 agoutis at different stages of the estrous cycle and four in the final trimester of pregnancy. Specimens were euthanized and their reproductive organs were fixed in a 4% paraformaldehyde or 2.5% glutaraldehyde solution and submitted to routine histological techniques for light and scanning electron microscopy. In the ovary, during the proestrus phase, we observed developing follicles and corpus luteum (CL) in regression; during estrus, there were Graafian follicles; during metestrus, there was a hemorrhagic corpus, whereas in diestrus, there was a mature CL. The uterus was partially double because the cervix was cranially septate but caudally, the septum disappeared, forming a single ostium that opened into the vagina. Changes occurred along the estrous cycle in the uterine and vaginal epithelia, that is, an increase in the uterine epithelium height accompanied by an increase of thickness of the vaginal epithelium during the follicular phase and a decrease of thickness of both epithelia during the luteal phase. The endometrial lining was composed of a simple cuboidal epithelium to simple columnar epithelium with basal nuclei. The vaginal mucosa consisted of epithelium that varied from nonkeratinized stratified squamous (luteal phase) to keratinized stratified squamous (follicular phase). The clitoris was external to the vagina. It presented two protruding lateral keratinized spicules and a centralized urethra, with no common parts between the urinary and genital tracts. Anatomical and histological changes were observed mainly in the cervix, vagina and spicules of the clitoris during the EC.

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Section: Discussionmentioning

confidence: 87%

Morphology of the genital organs of the female red‐rumped agouti (Dasyprocta leporina, Linnaeus, 1758) during estrous cycle phases and in advanced pregnancy

Oliveira

Júnior

Sousa³

et al. 2019

Journal of Morphology

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“…The agouti is a rodent that has been extensively studied , Menezes et al 2003, Azevêdo et al 2008) and which shows biological and physiological characteristics favorable for use in experimental research as a animal model (Fortes et al 2005, Conde-Júnior et al 2012, Rocha et al 2012. Bone marrow mononuclear cells of this animal were injected to treat induced renal injury (Cabral et al 2012) and in culture undifferentiated cells were obtained through the isolation and expansion by several passages (Rocha et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Isolation, expansion and differentiation of cellular progenitors obtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831)

et al. 2015

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The study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37⁰C. The growth curve, CFU assay, osteogenic/adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue.

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“…Therefore, obtaining information regarding reproduction of this species will also provide information for their rational production and consequent preservation [11]. In this regard, several studies have been performed involving the reproductive characteristics of agoutis, such as placentation [10], morphology of the ovaries [12] and oviducts [13], semen collection, and semen cryopreservation [14,15].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Wanderley¹,

Luz²,

Faustino³

et al. 2012

Theriogenology

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].You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol AbstractThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing, all samples were fixed in Carnoy prior to histological analysis. To evaluate ultrastructure, follicles from the control and all cryopreserved treatments were fixed in Karnovsky and processed for transmission electron microscopy. After exposure and freezing, there was a significant decrease in the percentage of morphologically normal preantral follicles in all treatments when compared to the control (92.67 ± 2.79, mean ± SD). However, there were no significant difference when the exposure and freezing procedures were compared using the same cryoprotectant. Moreover, there was no significant difference among cryoprotectants at the time of exposure (DMSO: 64.7 ± 3.8; EG: 70.7 ± 11.2, PROH: 63.3 ± 8.5) or after freezing (DMSO: 60.6 ± 3.6, EG: 64.0 ± 11.9; PROH: 62.0 ± 6.9). However, only follicles frozen with PROH had normal ultrastructure. In conclusion, preantral follicles enclosed in agouti ovarian tissue were successfully cryopreserved using 1.5 M PROH, with satisfactory maintenance of follicle morphology and ultrastructure.

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Aspectos morfológicos da tuba uterina de cutias (Dasyprocta aguti, Mammalia: Rodentia)

Cited by 8 publications

References 4 publications

Morphology of the genital organs of the female red‐rumped agouti (Dasyprocta leporina, Linnaeus, 1758) during estrous cycle phases and in advanced pregnancy

Morphology of the genital organs of the female red‐rumped agouti (Dasyprocta leporina, Linnaeus, 1758) during estrous cycle phases and in advanced pregnancy

Isolation, expansion and differentiation of cellular progenitors obtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831)

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

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