Aspects of 4-Hydroxy-pentenale on nucleic acid biosynthesis

Seeber, S.; Warnecke, P.; Weser, Ulrich

doi:10.1007/bf00524769

Z Krebs-forsch

1969

DOI: 10.1007/bf00524769

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Aspects of 4-Hydroxy-pentenale on nucleic acid biosynthesis

S. Seeber

P. Warnecke

Ulrich Weser

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1976

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Cited by 11 publications

(1 citation statement)

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“…The 4-hydroxyalkenals have especially interesting biological reactivities: 4-hydroxyoctenal, for example, can exert strongly inhibitory effects on various enzyme reactions (Schauenstein, 1967). The related 4-hydroxypentenal exerts strongly inhibitory effects on DNA and protein synthesis (Seeber et al, 1969;Dostal et al, 1974;Dianzani, 1979), reacts rapidly with thiols such as cysteine (Esterbauer et al, 1976) and glutathione (Esterbauer et al, 1975) and has inhibitory actions on certain tumour cell populations both in vitro and in vivo (for detailed references, see Schauenstein et al, 1977;Schauenstein & Esterbauer, 1979;Conroy et al, 1975).…”

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confidence: 99%

Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes

Esterbauer¹,

Cheeseman²,

Dianzani³

et al. 1982

Biochemical Journal

593

198

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1. Methods using t.l.c. and high-pressure liquid chromatography (h.p.l.c.) have been used to separate the complex variety of substances possessing a carbonyl function that are produced during lipid peroxidation. 2. The major type of lipid peroxidation studied was the ADP-Fe2+-stimulated peroxidation of rat liver microsomal phospholipids. Preliminary separation of the polar and non-polar products was achieved by t.l.c.: further separation and identification of individual components was performed by h.p.l.c. Estimations were performed on microsomal pellets and the supernatant mixture after incubation of microsomes for 30 min at 37 degrees C. 3. The polar fraction was larger than the non-polar fraction when expressed as nmol of carbonyl groups/g of liver. In the non-polar supernatant fraction the major contributors were n-alkanals (31% of the total), alpha-dicarbonyl compounds (22%) and 4-hydroxyalkenals (37%) with the extraction method used. 4. Major individual contributors to the non-polar fraction were found to be propanal, 4-hydroxynonenal, hexanal and oct-2-enal. Other components identified include butanal, pent-2-enal, hex-2-enal, hept-2-enal, 4-hydroxyoctenal and 4-hydroxyundecenal. The polar carbonyl fraction was less complex than the non-polar fraction, although the identities of the individual components have not yet been established. 5. Since these carbonyl compounds do not react significantly in the thiobarbituric acid reaction, which largely demonstrates the presence of malonaldehyde, it is concluded that considerable amounts of biologically reactive carbonyl derivatives are released in lipid peroxidation and yet may not be picked up by the thiobarbituric acid reaction.

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mentioning

confidence: 99%

Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes

Esterbauer¹,

Cheeseman²,

Dianzani³

et al. 1982

Biochemical Journal

593

198

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Zusammenhang zwischen den Protein-Thiolen und der Dopplungszeit verschiedener Ratten-Hepatome

Carow

Kharrazi

Khoschsorur

et al. 1982

J Cancer Res Clin Oncol

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The SH content of the soluble proteins (nanomol./mg protein) from five transplantable rat hepatomas and from the DENA-hepatoma were determined with dithionitrobenzoate (Ellman reagent). Both the total number of thiols as well as the number of SH groups that can be blocked by hydroxypentenal (HPE) increase with increasing growth rate of the tumors. In comparison with the protein thiol content of the slowest growing DENA-hepatoma (doubling time 100 days), the total protein thiols of the fastest growing Yoshida hepatoma (doubling time 2,5 days) increase by 100% and the HPE-sensitive protein thiols by 350%. The total protein thiols are significantly correlated with the growth rate (probability of error 5%), the HPE-sensitive thiols are correlated with high significance (probability of error less than 1%). These results are in accordance with the "Molecular Correlation Concept" of G. Weber and might possibly be understood as a consequence of reprogramming of gene expressions.

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Detection of 4-hydroxynonenal as a product of lipid peroxidation in native ehrlich ascites tumor cells

Winkler

Lindner

Esterbauer

et al. 1984

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Aspects of 4-Hydroxy-pentenale on nucleic acid biosynthesis

Cited by 11 publications

References 12 publications

Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes

Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADP-Fe2+ in rat liver microsomes

Zusammenhang zwischen den Protein-Thiolen und der Dopplungszeit verschiedener Ratten-Hepatome

Detection of 4-hydroxynonenal as a product of lipid peroxidation in native ehrlich ascites tumor cells

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