ASPECTS OF PATHOGENESIS IN A MOUSE MODEL OF INFECTION BY CORYNEBACTERIUM PSEUDOTUBERCULOSIS

Abstract

Summary.A new medium, nutrient tween 80 broth (NTB) was utilised to achieve improved cultivation of Corynebacterium pseudotuberculosis. Twenty four hour NTB cultures were used for cutaneous and intraperitoneal infection of mice. The intraperitoneal administration of heterologous protein, liquid paraffin or thioglycollate prior to intraperitoneal challenge caused enhanced multiplication of the organism although this could be prevented by modifying host response. Pretreatment of skin with thioglycollate enabled cutaneous infection to be consistently achieved. The progress of lesions in skin and viscera is described. Immunisation of the mice was effected by both intraperitoneal and cutaneous routes of inoculation, the latter being particularly useful because it did not lead to residual lesions of viscera. Immunity was expressed by a failure of organisms to localise at secondary sites although infection of primary sites in skin or peritoneal cavity occurred, apparently at a reduced level. The relevance of these findings to the natural disease of sheep is discussed.

Immunization with Recombinant Corynebacterium pseudotuberculosis Heat-Shock Protein (Hsp)-60 is Able to Induce an Immune Response in Mice, But Fails to Confer Protection Against Infection

“…BALB/c females, 6-8 weeks old, were obtained from the Federal University of Minas Gerais (UFMG) animal facility. This mouse strain was chosen due to its susceptibility to C. pseudotuberculosis infection [27][28].…”

Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain

“…The pathogenesis of CL in mice was demonstrated by monitoring the progression of lesions in the skin and viscera of infected animals [9]. Moreover, the physiology, pathogenicity and virulence mechanisms of C. pseudotuberculosis strains have been elucidated using genomics [8, 10], transcriptomics and proteomics methodologies [11, 12].…”

A case of kidney infection by Corynebacterium pseudotuberculosis in sheep

“…Ingestion is a more rare way of infection and it is apparent only in lesions to nodes of the buccal cavity. Inhalation can produce lung abscesses, while parasitic wounds are considered to be extremely rare as a portal of infection (Batey 1986b, Baird and Fontaine 2007, Radostits 2007. CLA has been reproduced in sheep by intradermal, subcutaneous, intravenous, intratracheal, intravaginal and intralymphatic route (Pepin et al 1994, Fontaine et al 2006, Baird and Fontaine 2007.…”