Aspergillus saccharolyticus sp. nov., a black Aspergillus species isolated in Denmark

Sørensen, Annette; Lübeck, Peter Stephensen; Lübeck, Mette; Nielsen, Kristian Fog; Ahring, Birgitte Kiær; Teller, Philip J.; Frisvad, Jens Christian

doi:10.1099/ijs.0.029884-0

Cited by 31 publications

(20 citation statements)

References 30 publications

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“…The identification and characterization of new fungal species are often encountered in literature. Within the black Aspergilli, to which several efficient beta-glucosidase products belong, several new species have been identified within recent years [94,95,96,97,98,99,100,101,102]. Screening for new enzymes can be performed at the genomic as well as the proteomic level—in either case, it can be a mixed gene or protein pool or a sample representing a specific species.…”

Section: New and Improved Beta-glucosidasesmentioning

confidence: 99%

Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

et al. 2013

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Profitable biomass conversion processes are highly dependent on the use of efficient enzymes for lignocellulose degradation. Among the cellulose degrading enzymes, beta-glucosidases are essential for efficient hydrolysis of cellulosic biomass as they relieve the inhibition of the cellobiohydrolases and endoglucanases by reducing cellobiose accumulation. In this review, we discuss the important role beta-glucosidases play in complex biomass hydrolysis and how they create a bottleneck in industrial use of lignocellulosic materials. An efficient beta-glucosidase facilitates hydrolysis at specified process conditions, and key points to consider in this respect are hydrolysis rate, inhibitors, and stability. Product inhibition impairing yields, thermal inactivation of enzymes, and the high cost of enzyme production are the main obstacles to commercial cellulose hydrolysis. Therefore, this sets the stage in the search for better alternatives to the currently available enzyme preparations either by improving known or screening for new beta-glucosidases.

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Section: New and Improved Beta-glucosidasesmentioning

confidence: 99%

Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

et al. 2013

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“…Since A . saccharolyticus is one of the best β-glucosidase producers known 22, 23 , it should be investigated whether A . labruscus is also an effective producer of such enzymes.…”

Section: Resultsmentioning

confidence: 99%

Aspergillus labruscus sp. nov., a new species of Aspergillus section Nigri discovered in Brazil

Fungaro

Ferranti

Massi

et al. 2017

Sci Rep

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A novel fungal species, Aspergillus labruscus sp. nov., has been found in Brazil during an investigation of the fungal species present on the surface of grape berries (Vitis labrusca L.) for use in the production of concentrated grape juice. It seems to be associated to V. labrusca, and has never been recovered from Vitis vinifera. This new species belonging to Aspergillus subgenus Circumdati section Nigri is described here using morphological characters, extrolite profiling, partial sequence data from the BenA and CaM genes, and internal transcribed spacer sequences of ribosomal DNA. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other black aspergilli. A. labruscus sp. nov. is uniseriate, has yellow mycelium, poor sporulation on CYA at 25 °C, abundant salmon to pink sclerotia and rough conidia. Neoxaline and secalonic acid D were consistently produced by isolates in this taxon. The type strain of A. labruscus sp. nov. is CCT 7800 (T) = ITAL 22.223 (T) = IBT 33586 (T).

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“…However, besides of this known strain, one of our own isolates, strain AP, showed a very promising profile in terms of onsite enzyme production using FC as growth medium. This strain AP (CBS 127449) has recently been characterized as a novel Aspergillus species: Aspergillus saccharolyticus [28]. Stain AP was able to compete with the reference strain and was sufficient for hydrolysis of WO in combination with Celluclast 1.5L.…”

Section: Synergies With Commercial Enzymesmentioning

confidence: 99%

Onsite Enzyme Production During Bioethanol Production from Biomass: Screening for Suitable Fungal Strains

Sørensen

Teller

Lübeck

et al. 2011

Appl Biochem Biotechnol

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Cellulosic ethanol production from biomass raw materials involves process steps such as pre-treatment, enzymatic hydrolysis, fermentation, and distillation. Use of streams within cellulosic ethanol production was explored for onsite enzyme production as part of a biorefinery concept. Sixty-four fungal isolates were in plate assays screened for lignocellulolytic activities to discover the most suitable fungal strain with efficient hydrolytic enzymes for lignocellulose conversion. Twenty-five were selected for further enzyme activity studies using a stream derived from the bioethanol process. The filter cake left after hydrolysis and fermentation was chosen as substrate for enzyme production. Five of the 25 isolates were further selected for synergy studies with commercial enzymes, Celluclast 1.5L and Novozym 188. Finally, IBT25747 (Aspergillus niger) and strain AP (CBS 127449, Aspergillus saccharolyticus) were found as promising candidates for onsite enzyme production where the filter cake was inoculated with the respective fungus and in combination with Celluclast 1.5 L used for hydrolysis of pre-treated biomass.

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Aspergillus saccharolyticus sp. nov., a black Aspergillus species isolated in Denmark

Cited by 31 publications

References 30 publications

Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials

Aspergillus labruscus sp. nov., a new species of Aspergillus section Nigri discovered in Brazil

Onsite Enzyme Production During Bioethanol Production from Biomass: Screening for Suitable Fungal Strains

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