Asperosaponin VI promotes progesterone receptor expression in decidual cells via the notch signaling pathway

Gao, Jie; Zhou, Chun; Li, Yadi; Gao, Feixia; Wu, Hui; Liu, Yang; Qiu, Weiyu; Zhu, Lin; Du, Xin; Lin, Weixian; Huang, Dandan; Liu, Haibin; Liang, Chun; Luo, Songping

doi:10.1016/j.fitote.2016.06.013

Cited by 13 publications

(12 citation statements)

References 29 publications

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“…, is an important and common traditional Chinese medicine, has a long application history on clinical. The current research showed DR has multiple pharmaco-activities, and the main effective part is the saponins ( Gao et al, 2016 ). Among them, asperosaponin VI (ASA VI) is the one of the highest content of saponins ( Ding et al, 2019 ).…”

Section: Introductionmentioning

confidence: 97%

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Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

Zhang

Xiao

et al. 2020

Saudi Journal of Biological Sciences

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Microglia cells are the main mediators of neuroinflammation. Activation of microglia often aggravates the pathological process of various neurological diseases. Natural chemicals have unique advantages in inhibiting microglia-mediated neuroinflammation and improving neuronal function. Here, we examined the effects of asperosaponin VI (ASA VI) on LPS-activated primary microglia. Microglia were isolated from mice and pretreated with different doses of ASA VI, following lipopolysaccharide (LPS) administration. Activation and inflammatory response of microglia cells were evaluated by real-time fluorescence quantitative polymerase chain reaction (q-PCR), immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Signaling pathways were detected by western blotting. We found that the ASA VI inhibited the morphological expansion of microglia cells, decreased the expression and release of proinflammatory cytokines, and promoted the expression of antiinflammatory cytokines in a dose-dependent manner. ASA VI also activated PPAR-γ signaling pathway in LPS-treated microglia. The anti-inflammatory effects of ASA VI in microglia were blocked by treating PPAR-γ antagonist (GW9662). These results showed that ASA VI promote the transition of microglia cells from proinflammatory to anti-inflammatory by regulating PPAR-γ pathway.

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Section: Introductionmentioning

confidence: 97%

“…Among them, asperosaponin VI (ASA VI) is the one of the highest content of saponins ( Ding et al, 2019 ). The effects of ASA VI on proliferation and differentiation of osteoblasts has been widely reported ( Gao et al, 2016 , Liu et al, 2019 ). However, the role of ASA VI in innate immune regulation is unclear.…”

Section: Introductionmentioning

confidence: 99%

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

Zhang

Xiao

et al. 2020

Saudi Journal of Biological Sciences

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“…Isolation, culture, and identification of primary decidual cells were performed according to protocols published in previous studies [ 22 ]. All patients gave their written informed consent according to Declaration of Helsinki, and we obtained the permission of Ethics Committee of The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine (No.…”

Section: Methodsmentioning

confidence: 99%

Effect of Total Flavones from Cuscuta Chinensis on Anti‐Abortion via the MAPK Signaling Pathway

Feng

Wang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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For centuries, the Chinese herb Cuscuta chinensis has been applied clinically for abortion prevention in traditional Chinese medicine (TCM). Total flavones extracted from Cuscuta chinensis (TFCC) are one of the active components in the herb and also display anti-abortion effect similar to the unprocessed material. However, how TFCC exerts the anti-abortion effect remains largely unknown. In this study, we aim at characterizing the anti-abortion effects of TFCC and its underlying molecular mechanism in vitro and in vivo using human primary decidua cells and a mifepristone-induced abortion model in rat, respectively. The damage to the decidua caused by mifepristone in vivo was reversed by TFCC treatment in a dosage-dependent manner. High dosage of TFCC significantly upregulated the expression of estrogen receptor (ER), progesterone receptor (PR), and prolactin receptor (PRLR) in decidua tissue but downregulated the expression of p-ERK. Furthermore, we detected higher level of p-ERK and p-p38 in primary decidua cells from spontaneous abortion while treatment by TFCC downregulated their expression. Our results suggest TFCC mediates its anti-abortion effect by interfering with MAPK signaling pathway.

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“…Dipsaci Radix (DR), the desiccative radix of Dipsacus asper., is an important and common traditional Chinese medicine, has a long application history on clinical. The current research showed DR has multiple pharmaco-activities, and the main effective part is the saponins (Gao et al, 2016). Among them, one of the highest content of saponins is asperosaponin Ⅵ (ASA Ⅵ) .…”

Section: Introductionmentioning

confidence: 99%

“…Among them, one of the highest content of saponins is asperosaponin Ⅵ (ASA Ⅵ) . The effect of ASA Ⅵ on proliferation and differentiation of osteoblasts has been widely reported (Gao et al, 2016. However, the role of ASA Ⅵ in innate immune regulation is unclear.…”

Section: Introductionmentioning

confidence: 99%

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

Zhang

Xiao

et al. 2020

Preprint

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Microglia cells are the main mediators of neuroinflammation.Activation of microglia often aggravates the pathological process of various neurological diseases. Natural chemicals have unique advantages in inhibiting microglia-mediated neuroinflammation and improving neuronal function. Here, we examined the effects of asperosaponin Ⅵ (ASA Ⅵ) on LPS-activated primary microglia. Microglia were isolated from mice and pretreated with different doses of ASA Ⅵ, following lipopolysaccharide (LPS) administration. Activation and inflammatory response of microglia cells were evaluated by q-PCR, immunohistochemistry and ELISA. Signaling pathways were detected by western blotting. We found that the ASA Ⅵ inhibited the morphological expansion of microglia cells, decreased the expression and release of proinflammatory cytokines, and promoted the expression of antiinflammatory cytokines in a dose-dependent manner. ASA Ⅵ also activated PPAR-γ signaling pathway in LPS-treated microglia. The anti-inflammatory effects of ASA Ⅵ in microglia were blocked by treating PPAR-γ antagonist (GW9662). These results showed that ASA Ⅵ promote the transition of microglia cells from proinflammatory to anti-inflammatory by regulating PPAR-γ pathway.

show abstract

Asperosaponin VI promotes progesterone receptor expression in decidual cells via the notch signaling pathway

Cited by 13 publications

References 29 publications

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

Effect of Total Flavones from Cuscuta Chinensis on Anti‐Abortion via the MAPK Signaling Pathway

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

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