Asperosaponin VI protects cardiac myocytes from hypoxia-induced apoptosis via activation of the PI3K/Akt and CREB pathways

Li, Chunmei; Tian, Jingwei; Li, Guisheng; Jiang, Wanglin; Xing, Yanli; Hou, Jian; Zhu, Haibo; Hong, Xu; Zhang, Guanbo; Liu, Zhifeng; Ye, Zuguang

doi:10.1016/j.ejphar.2010.08.060

Cited by 44 publications

(30 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In further experiments, we studied effects of Sox8 on the PI3K/AKT/mTOR signal pathway and MAPK activation. PI3K/AKT signal pathway is an important pathway for protecting myocardial cells against hypoxia-induced injury [35, 36]. Xiao et al reported that hydrogen sulfide protects myocardial cells against hypoxia-induced injury via mTOR activation [37].…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Gong¹,

Xu²,

Guo³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time; ischemia is primarily caused due to hypoxia. The present study evaluated the effects of long non-coding RNA H19 on hypoxic rat H9c2 cells and mouse HL-1 cells. Methods: Hypoxic injury was confirmed by measuring cell viability, migration and invasion, and apoptosis using MTT, Transwell and flow cytometry assays, respectively. H19 expression after hypoxia was estimated by qRT-PCR. We then measured the effects of non-physiologically expressed H19, knockdown of miR-139 with or without H19 silence, and abnormally expressed Sox8 on hypoxia-induced H9c2 cells. Moreover, the interacted miRNA for H19 and downstream target gene were virtually screened and verified. The involved signaling pathways and the effects of abnormally expressed H19 on contractility of HL-1 cells were explored via Western blot analysis. Results: Hypoxia induced decreases of cell viability, migration and invasion, increase of cell apoptosis and up-regulation of H19. Knockdown of H19 increased hypoxia-induced injury in H9c2 cells. H19 acted as a sponge for miR-139 and H19 knockdown aggravated hypoxia-induced injury by up-regulating miR-139. Sox8 was identified as a target of miR-139, and its expression was negatively regulated by miR-139. The mechanistic studies revealed that overexpression of Sox8 might decrease hypoxia-induced cell injury by activating the PI3K/AKT/mTOR pathway and MAPK. Besides, H19 promoted contractility of HL-1 cells. Conclusion: These findings suggest that H19 alleviates hypoxia-induced myocardial cell injury by miR-139-mediated up-regulation of Sox8, along with activation of the PI3K/AKT/mTOR pathway and MAPK.

show abstract

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Gong¹,

Xu²,

Guo³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Thus, the ratio of Bcl-2 to Bax may be a critical factor in the cellular threshold for apoptosis (Tamm et al, 2001). In addition, caspase cascade also plays a key role in apoptosis (Li et al, 2010;Lakhani et al, 2006). Activation of caspase-3 results in cleavage of cytoskeletal and nuclear proteins, and nucleosomal fragmentation of DNA (Nicholson et al, 1995).…”

Section: Discussionmentioning

confidence: 98%

Cardioprotective effect of Danshensu against myocardial ischemia/reperfusion injury and inhibits apoptosis of H9c2 cardiomyocytes via Akt and ERK1/2 phosphorylation

Yin

Guan

Duan

et al. 2013

European Journal of Pharmacology

135

105

View full text Add to dashboard Cite

“…The MTT assay was performed according to a published method. 27) Each experiment was performed at least in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Astragaloside IV Inhibits Doxorubicin-Induced Cardiomyocyte Apoptosis Mediated by Mitochondrial Apoptotic Pathway <i>via</i> Activating the PI3K/Akt Pathway

Jia

Zuo

et al. 2014

Chem. Pharm. Bull.

View full text Add to dashboard Cite

Asperosaponin VI protects cardiac myocytes from hypoxia-induced apoptosis via activation of the PI3K/Akt and CREB pathways

Cited by 44 publications

References 34 publications

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139

Cardioprotective effect of Danshensu against myocardial ischemia/reperfusion injury and inhibits apoptosis of H9c2 cardiomyocytes via Akt and ERK1/2 phosphorylation

Astragaloside IV Inhibits Doxorubicin-Induced Cardiomyocyte Apoptosis Mediated by Mitochondrial Apoptotic Pathway <i>via</i> Activating the PI3K/Akt Pathway

Contact Info

Product

Resources

About