Aspirin attenuates vinorelbine-induced endothelial inflammation via modulating SIRT1/AMPK axis

Quercetin is a potent anti‐atherosclerotic compound by activation of SIRT1 signaling under oxLDL stimulation

“…Quercetin was shown to be a potent AMPK agonist in a previous study . Upregulating the SIRT1/AMPK axis has been an effective strategy to inhibit oxidative injury induced endothelial dysfunction . Blocking AMPK activity activates protein kinase C (PKC), thereby facilitating intracellular ROS formation and oxidative stress augmentation .…”

“…In general, reactive oxygen species (ROS) derived oxidative stress plays a key role in inducing endothelial dysfunction . In human endothelial cells, the NADPH oxidase system is critical for ROS formation under oxidative stimulation and is a primary facilitator of the progression of endothelial dysfunction . High concentrations of free radicals impair NO bioavailability and reduce telomere length, thereby causing apoptosis, DNA injury, and endothelial aging .…”

Aspirin regulates hepatocellular lipid metabolism by activating AMPK signaling pathway

“…HepG2 cells (human hepatoma cells) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Beijing, China) and cultured in 90% DMEM/F12 with 2 mM L-glutamine and supplemented with 10% fetal bovine serum, and 2% penicillin-streptomycin (10,000 U/mL penicillin and 10 mg/mL streptomycin), at 37°C and in a 5% humidified CO2 atmosphere. For the aspirin treatment experiment, HepG2 cells were grown to 85% confluence and subjected to 90% DMEM/F12 supplemented with 2% FBS and containing various concentrations of aspirin or AIC-AR, according to a previous report (Tsai et al, 2014). At various hours post treatment, HepG2 cells were lysed to RNA or protein isolation.…”

“…Professor Grahame Hardie and his colleagues have proven that salicylate directly activated AMPK signaling, by binding to the same site of the synthetic activator A-769662 to cause allosteric activation and inhibition of dephosphorylation of the activating phosphorylation site, threonine-172 of AMPK (Hawley et al, 2012). Recently, aspirin has been well recognized to repress TGF-β-activated kinase-1 (TAK1) activation by modulating Silent Information Regulator T1 (SIRT1)/AMPK axis, to inhibit the interaction of TAK1/TAK-binding protein1 (TAB1), and then to suppress the NF-κB activation and pro-inflammatory cytokine secretion (Tsai et al, 2014). Given the wellconfirmed regulatory role of aspirin in lipid metabolism and its anti-atherosclerotic effect, and the recently recognized modulation by aspirin in the AMPK signaling pathway, aspirin might perform its regulation in lipid metabolism via the AMPK signaling pathway.…”

Nicotinic acid inhibits vascular inflammation via the SIRT1-dependent signaling pathway

“…Therefore, we wondered whether the potential mechanisms of NA were related to the expression of SIRT1. A recent report revealed that aspirin attenuated IL-6 secretion via up-regulating SIRT1 expression in vinorelbine-treated endothelial cells [34]. Our present study showed that the stimulation of collar placement leaded to the decrease of SIRT1 expression in the arteries of rabbits.…”