Assay and purity control of tetracycline, chlortetracycline and oxytetracycline in animal feeds and premixes by TLC densitometry with fluorescence detection

Weng, Naidong; Hua, Sun; Roets, Eugène; Hoogmartens, Jos

doi:10.1016/s0731-7085(03)00153-5

Cited by 49 publications

(22 citation statements)

References 13 publications

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“…Several analytical procedures are currently available for the determination of TC residues in animal tissues and at present chromatographic techniques are mainly used as confirmatory ones for these substances. Despite the simplicity and the low cost of TLC [14,15], HPLC coupled with UV [9, 16 -18], fluorescence [13,19], and MS [20 -23] remains the technique of choice for multiresidue analyses. Isolation of TCs from tissues includes an extraction step with a suitable solvent system and a clean-up procedure.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2005

J of Separation Science

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A high-performance liquid chromatographic method with diode-array detection, at 351 nm, was developed and validated for the determination of five tetracyclines (TCs): minocycline, tetracycline, oxytetracycline, chlortetracycline, and doxycycline in bovine muscle. Samples were macerated with a buffer solution, centrifuged, and purified using Abselut Nexus SPE cartridges. The separation of the examined TCs was achieved on an Inertsil ODS-3 5 microm, 250 x 4 mm analytical column, at ambient temperature. A multistep gradient elution was followed using 0.05 M oxalic acid and CH3CN, at a flow rate of 1.65 mL/min. The procedure was validated according to the European Union regulation 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. The results of the validation process demonstrate that the method can be readily applied to European Union statutory veterinary drug residue surveillance programmes. Mean recoveries of TCs from bovine muscle samples spiked at three concentrations (100, 250, and 400 ng/g) were in the range of 98.7-103.3%. Method's LOQ values achieved were 40 microg/kg for MNC, CTC, and DC and 25 microg/kg for OTC and TC. The decision limits (CCalpha) were in the range of 104.7-109.8 microg/kg, while the detection capability (CCbeta) was in the range of 108.4-116.7 microg/kg for all compounds.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2005

J of Separation Science

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“…Unmodified Silica gel 60 F 254 [92][93][94][95][96], Silica gel GF 254 [97], and Silica gel 60 H [98] are commonly used as the sorbent. Reversed-phase plates coated with silica gel with modifying -CN [97], C 8 , and C 18 [98] groups and cellulose-based sorbents [99,100] have been used in some works.…”

Section: Determination Of Organic Impurities In Pharmaceutical Preparmentioning

confidence: 99%

“…Reversed-phase plates coated with silica gel with modifying -CN [97], C 8 , and C 18 [98] groups and cellulose-based sorbents [99,100] have been used in some works. Silufol and Sorbfil plates are widely used in Russia [101,102].…”

Section: Determination Of Organic Impurities In Pharmaceutical Preparmentioning

confidence: 99%

Determination of organic impurities in pharmaceutical preparations

Glazkov

Bochkareva²,

Revel'skii

2005

J Anal Chem

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“…For the determination of methacycline hydrochloride, high performance liquid chromatography [8] and antibiotics-microbial assay [9] were recommended in some pharmacopoeias. Only few studies on the method for determination of methacycline(MC) in pharmaceutical analysis are reported, including high performance liquid chromatography [10], spectrophotometry [11], phosphorimetry [12] and thin-layer chromatography [13][14]. Ion-selective electrodes are playing an important role in pharmaceutical analysis [15][16][17] due to its simplicity, rapidity and accuracy over some other analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

Aboul‐Enein

Sun

2002

Sensors

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A plastic membrane electrode for the determination of methacycline hydrochloride was fabricated based on the use of methacycline-tetraphenylborate as the electroactive substance, and dioctylphtalate as the plasticizing agent. It was found that the potential responses of the electrode were influenced by pH of the tested solution when pH>3. Under the condition of pH 2.6, the linear response range, slope (25ºC) and detection limit obtained were 3.0×10 -2~6.0×10-6 M, 52.9mv per decade and 3.4×10 -6 M ,respectively.The response time of the electrodes was <15 seconds. The values of recovery obtained were 99.2~101.2%. The electrode was successfully applied to determination of methacycline hydrochloride in tablet by direct potentiometric method. The result obtained with the electrode was in good agreement with the value obtained by using the official method (highperformance liquid chromatography).

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Assay and purity control of tetracycline, chlortetracycline and oxytetracycline in animal feeds and premixes by TLC densitometry with fluorescence detection

Cited by 49 publications

References 13 publications

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Determination of organic impurities in pharmaceutical preparations

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

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