2006
DOI: 10.1373/clinchem.2005.060038
|View full text |Cite
|
Sign up to set email alerts
|

Assay for the Measurement of Copeptin, a Stable Peptide Derived from the Precursor of Vasopressin

Abstract: Background: Arginine vasopressin (AVP) is a key regulator of water balance, but its instability makes reliable measurement difficult and precludes routine use. We present a method for quantifying AVP release by use of copeptin, a glycopeptide comprising the C-terminal part of the AVP prohormone. Methods: We measured copeptin in 50-L serum and plasma samples from healthy individuals and from critically ill patients with sepsis. Our sandwich immunoluminometric assay used 2 polyclonal antibodies to amino acids 13… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

23
883
12
12

Year Published

2009
2009
2019
2019

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 936 publications
(930 citation statements)
references
References 24 publications
23
883
12
12
Order By: Relevance
“…UAE, given as the mean of the two 24 h urine excretions, was determined by nephelometry with a threshold of 2.3 mg/l and intra-and inter-assay coefficients of variation of less than 2.2% and less than 2.6%, respectively (Dade Behring Diagnostic, Marburg, Germany). Plasma copeptin level was measured using a new sandwich immunoassay (B.R.A.H.M.S GmbH/Thermo Fisher Scientific, Hennigsdorf/Berlin, Germany), which has been described previously [5,20]. The lower detection limit was 0.4 pmol/ l and the functional assay sensitivity (20% inter-assay coefficient of variation) was less than 1 pmol/l [5].…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…UAE, given as the mean of the two 24 h urine excretions, was determined by nephelometry with a threshold of 2.3 mg/l and intra-and inter-assay coefficients of variation of less than 2.2% and less than 2.6%, respectively (Dade Behring Diagnostic, Marburg, Germany). Plasma copeptin level was measured using a new sandwich immunoassay (B.R.A.H.M.S GmbH/Thermo Fisher Scientific, Hennigsdorf/Berlin, Germany), which has been described previously [5,20]. The lower detection limit was 0.4 pmol/ l and the functional assay sensitivity (20% inter-assay coefficient of variation) was less than 1 pmol/l [5].…”
Section: Methodsmentioning
confidence: 99%
“…Plasma copeptin level was measured using a new sandwich immunoassay (B.R.A.H.M.S GmbH/Thermo Fisher Scientific, Hennigsdorf/Berlin, Germany), which has been described previously [5,20]. The lower detection limit was 0.4 pmol/ l and the functional assay sensitivity (20% inter-assay coefficient of variation) was less than 1 pmol/l [5]. All the technicians were blinded to the participants' characteristics.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The advantage of copeptin is that it is more stable in blood circulation and easier to measure compared to AVP. 14 The secretion of AVP can be stimulated through brainstem and limbic pathways triggered by different "stressors", it seems to act as an endogenous barometer of integral homeostasis. Thus copeptin assesses the severity of damage beyond lesion size, the effect of age, gender, and measurable clinical impairment on admission.…”
Section: Discussionmentioning
confidence: 99%
“…In 359 healthy individuals, median copeptin levels were reported to be 4.2 pmol/l with a 99th percentile of 13.5 pmol/l. 14 …”
Section: Biomarker Measurementmentioning
confidence: 99%