ASSAY OF 17β-Oestradiol BY COMPETITIVE PROTEIN BINDING METHODS

Corker, C. S.; Exley, D.; Naftolin, F

doi:10.1530/acta.0.065s305

Cited by 20 publications

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“…The antiserum used was relatively specific for oestradiol with only a 5% cross-reaction with oestrone. Levels of immunoreactive oestrogens by this method corresponded closely with those obtained using a competitive protein-binding method specific for oestradiol (Corker, Exley, and Naftolin, 1970). The average coefficient of variance of oestradiol recovery from a pooled plasma sample was 6%.…”

Section: Methods Of Assay Plasma Luteinizing Hormone (Lh) This Wassupporting

confidence: 68%

Plasma levels of luteinizing hormone and of immunoreactive oestrogens (oestradiol) in anorexia nervosa: response to clomiphene citrate

1973

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SynopsisIn a study of 11 patients with anorexia nervosa, plasma levels of luteinizing hormone (LH) and of oestrogens (oestradiol) were found to be low while the subjects were emaciated. After weight gain, hormone values rose and two patients spontaneously resumed menstruation. Clomiphene citrate was administered on one or more occasions to five patients, and bleeding was induced in the majority of those whose weight exceeded 80% of standard. In two of these patients an irregular cycle resumed for a brief period thereafter. Clomiphene appeared to be of limited practical value in the mangement of patients with anorexia nervosa, but the study provided some elucidation of the relationship between body weight and the menstrual disturbance in this condition.

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Section: Methods Of Assay Plasma Luteinizing Hormone (Lh) This Wassupporting

confidence: 68%

Plasma levels of luteinizing hormone and of immunoreactive oestrogens (oestradiol) in anorexia nervosa: response to clomiphene citrate

1973

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“…These eluates were divided into three equal portions; one of these served for determining the recovery of the radioactive internal standard, and the other two for the CPB assay. The assay was done in duplicate according to the method of Korenman (1968), as modified by Corker, Exley «fe Naftolin (1970).…”

Section: Animals and Blood Collectionsmentioning

confidence: 99%

Oestradiol Levels in the Peripheral Blood of Cows During the Oestrous Cycle

Shemesh¹,

Ayalon²,

Lindner³

1972

Journal of Endocrinology

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Changes in the peripheral plasma level of oestradiol during the bovine oestrous cycle were determined by a competitive protein-binding assay, following chromatographic purification of the steroid. Oestradiol concentration increased during the 3 days preceding oestrus and attained its peak value [17 \m=+-\1\m=.\9 (s.e.m.) ng/100 ml] 4 h before oestrous behaviour could be detected. The level declined steeply during the day of oestrus to reach a nadir (0\m=.\8 \ m=+-\ 0\m=.\11) before the time of ovulation. A minor rise was observed on day 4 and a more sustained increase on days 10-13, with a peak on day 11 (8\m=.\1\ m=+-\ 3\m=.\6).Comparison with published data on luteinizing hormone (LH) levels suggests that the pre-oestrous surge of oestradiol precedes the pr e\ x=r eq-\ ovulatory surge of LH in the cow.

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“…After incubation with 20 nM-[3H]DHT for 20 h, the nuclear extracts were precipitated with protamine sulphate and filtered on Whatman GF/C filters. The filters were then extracted three times with méthylène dichloride and the extracted steroids were chromatographed on thin-layer alumina gel plates using benzene-ethanol (20/1, v/v) (Corker, Exley & Naftolin, 1970). At the end of incubation, Chromato¬ graphie analysis indicated that 74% of the [3H]DHT was unchanged.…”

Section: Methodsmentioning

confidence: 99%

Relationship of testosterone pulses to androgen binding in the pituitary of rams

Pelletier¹,

Terqui²,

Thieulant³

1985

Reproduction

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The effects of testosterone on cytosol and nuclear androgen receptors of ram pituitary were examined in two experiments. In Exp. I, 500 micrograms testosterone were injected intravenously and groups of 4 rams were slaughtered at 0, 15, 30, 45, 90 and 360 min after injection. Cytosolic receptor concentration decreased from 21 +/- 0.9 to 6 +/- 0.9 fmol/mg protein 30 min after the testosterone injection (P less than 0.001), and then returned towards the preinjection level after 90 min. The pattern of nuclear receptor concentration was the opposite; a maximal increase (12 +/- 3.5 to 32 +/- 5.7 fmol/mg protein) was observed 30 min after injection (P less than 0.001), followed by a progressive but incomplete decrease by 360 min. In Exp. II, blood was collected every 20 min for 17 h in three successive series, each of 12 rams, which were then slaughtered. Plasma LH and testosterone concentrations were measured by radioimmunoassay. No changes were observed in cytosol receptor concentration, but nuclear receptor concentration was negatively correlated with the interval elapsed since the beginning of the last testosterone pulse (r = -0.62; P less than 0.001). The highest values for nuclear receptor concentrations were observed at an interval equal to or less than 120 min. These results indicate that natural pulses are associated with androgen binding particularly in the pituitary nuclei.

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ASSAY OF 17β-Oestradiol BY COMPETITIVE PROTEIN BINDING METHODS

Cited by 20 publications

References 0 publications

Plasma levels of luteinizing hormone and of immunoreactive oestrogens (oestradiol) in anorexia nervosa: response to clomiphene citrate

Plasma levels of luteinizing hormone and of immunoreactive oestrogens (oestradiol) in anorexia nervosa: response to clomiphene citrate

Oestradiol Levels in the Peripheral Blood of Cows During the Oestrous Cycle

Relationship of testosterone pulses to androgen binding in the pituitary of rams

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