Assay of Human Leukoagglutinins by Capillary Migration

Thompson, John S.; Severson, Charles D.; Lavender, A R; Forland, Marvin; Russe, Henry P.

doi:10.1097/00007890-196808000-00005

Cited by 19 publications

(8 citation statements)

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“…Leucoagglutination of human buffy coat cells was measured using the method of Thompson et al [1968], Haemagglutination was measured by adding 0.2 ml of seed extract to 0.2 ml of a 3-percent (v/v) suspension of human erythrocytes on a glass slide. Agglutination was viewed macroscopically in daylight against a white background.…”

Section: Methodsmentioning

confidence: 99%

Lymphocyte Mitogens of Indigenous Australian Plant Species

Curtain¹,

Simons²

1972

Int Arch Allergy Immunol

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Saline extracts of seeds from 164 Australian indigenous plant species representing 73 genera and 28 families were tested for their ability to stimulate blast transformation and mitosis in human buffy coat cells. Extracts from 3 species, Rhagodia crassifolia, Bassia decurrens and Bauhinia Carronii were found to be active. The most active extract, from B. Carronii was fractionated by isoelectric focussing, by which it was found possible to separate the mitogenic, haemagglutinating and leucoagglutinating activities. The activity of the B. Carronii mitogen was not inhibited by antiserum to the phytohaemagglutinin of Phaseolus vulgaris (PHA) and its chemical composition and sedimentation co-efficient differed from those published for PHA and the mitogen of the pokeweed (Phytolacca americana).

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Section: Methodsmentioning

confidence: 99%

Lymphocyte Mitogens of Indigenous Australian Plant Species

Curtain¹,

Simons²

1972

Int Arch Allergy Immunol

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“…To detect anti-HLA antibodies developed in these patients, LCY test (14) was performed using antiserum obtained from the patient against a lymphocyte panel obtained from 50 HLA typed unrelated donors on which the HLA antigens were represented more than once. Leukocyte capillary agglutination was described previously (15).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of antibody response to human platelet transfusion.

Wu¹,

Thompson²,

Koepke³

et al. 1976

J. Clin. Invest.

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A B S T R A C T To study the antibody response to human platelet transfusions, nine thrombocytopenic patients with bone marrow failure were given 6 U (3 X 10") of random platelet concentrates twice a week.Before transfusion, none of the patients had preexisting antibodies detectable with lymphocytotoxicity, platelet aggregation, or capillary leukoagglutination techniques. After receiving 18-78 U of platelets, they became refractory to further transfusions of random platelets and alloantibodies were detectable. Two patterns of antibody response could be identified. In three patients, the sera were not lymphocytotoxic with a panel of standard cells in which all the known HLA antigens in the first and second series were represented at least once. Yet, they caused platelet aggregation with 30, 24, and 60%, respectively, of a donor population studied. The aggregating activities were inhibited by antihuman IgG but not by antihuman IgA or antihuman IgM antiserum. The aggregating antibodies could be absorbed out with donor platelets but not lymphocytes or granulocytes. Antibodies from two of these patients aggregated platelets of their respective siblings matched for both HLA haplotypes. Transfusion of platelets from these two siblings did not increase the platelet count while platelets obtained from aggregation-negative donors did. The sera from the remaining six patients were lymphocytotoxic with 15-100% of the panel of standard cells. They also had aggregating antibodies, which could be absorbed out by both platelets and lymphocytes, suggesting that they were HLA antibodies. These data suggest that the development of platelet-specific antibodies may play an Dr. Wu's current address is Section

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“…One microliter of fluorescein diacetate (Serva, Heidelberg, FRG) treated granulocyte suspension (3 X lo6/&) was added to 1 p1 of GF 26.7.3 supernatant or ascitic fluid at various dilutions in a Terasaki tray (Falcon 3034 B-D, Grenoble, France) and incubated for 30 min at room temperature. Then, 5 pl of non-granulocytotoxic, fresh rabbit serum as source of complement were added and after 60 min incubation, the tray was observed with an inverted fluorescence microscope (Thompson et al, 1968).…”

Section: Iif On Peripheral Blood Cells Bone-marrow Cells and Leukemimentioning

confidence: 99%

A monoclonal antibody to human transitional‐cell carcinoma of the bladder cross‐reacting with a differentiation antigen of neutrophilic lineage

Baricordi

Sensi

Vinci

et al. 1985

Intl Journal of Cancer

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A monoclonal antibody (MAb) to human transitional-cell carcinoma of the bladder (TCCB) was obtained by immunization of a BALB/c mouse with formalin-fixed TCCB cells and subsequent fusion of the spleen cells with SP2-OAg14 myeloma line. GF 26.7.3 MAb was selected by indirect immunofluorescence (IIF) as reacting agent with target cells and negative with autologous lymphocytes and Epstein-Barr virus (EBV)-transformed lymphoblastoid cell-line. GF 26.7.3 reacts with a high percentage of bladder and colon carcinomas when examined by IIF and immunoperoxidase techniques and cross-reacts with a determinant expressed on neutrophilic cell lineage. The IIF analysis performed on bone marrow and peripheral blood (PB) from healthy subjects and leukemic patients and on leukemic cell lines showed that the expression of the structure detected by GF 26.7.3 is restricted to the neutrophilic cell lineage and first expressed at the promyelocytic level. Immunoprecipitation and SDS-polyacrylamide gel electrophoresis (PAGE) of 125 I-labelled membrane proteins from target cells were performed, but no bands were detected by autoradiography. In addition, pronase insensitivity and periodate sensitivity suggest the possible involvement of a carbohydrate determinant.

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Assay of Human Leukoagglutinins by Capillary Migration

Cited by 19 publications

References 0 publications

Lymphocyte Mitogens of Indigenous Australian Plant Species

Lymphocyte Mitogens of Indigenous Australian Plant Species

Heterogeneity of antibody response to human platelet transfusion.

A monoclonal antibody to human transitional‐cell carcinoma of the bladder cross‐reacting with a differentiation antigen of neutrophilic lineage

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