Assay of Phospholipase A Activity

Heinze, Michael; Roos, Werner

doi:10.1007/978-1-62703-401-2_22

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Lysophospholipids and their metabolites are signaling molecules that are involved in cellular adaptation to stress, developmental processes, and various diseases in both plants and animals [ 93 ]. Current evidence suggests that PLA represents a crucial target involved in the modulation of intracellular molecular pathways, leading to the generation of lipid mediators, which control and regulate cell proliferation, survival, differentiation, motility, and tissue vascularization [ 94 ]. Therefore, the deregulation of PLA is involved in the pathogenesis of several diseases and cancer development and progression.…”

Section: Pla and Lung Cancermentioning

confidence: 99%

Phospholipase Family Enzymes in Lung Cancer: Looking for Novel Therapeutic Approaches

Salucci,

Aramini,

Bartoletti-Stella

et al. 2023

Cancers

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Lung cancer (LC) is the second most common neoplasm in men and the third most common in women. In the last decade, LC therapies have undergone significant improvements with the advent of immunotherapy. However, the effectiveness of the available treatments remains insufficient due to the presence of therapy-resistant cancer cells. For decades, chemotherapy and radiotherapy have dominated the treatment strategy for LC; however, relapses occur rapidly and result in poor survival. Malignant lung tumors are classified as either small- or non-small-cell lung carcinoma (SCLC and NSCLC). Despite improvements in the treatment of LC in recent decades, the benefits of surgery, radiotherapy, and chemotherapy are limited, although they have improved the prognosis of LC despite the persistent low survival rate due to distant metastasis in the late stage. The identification of novel prognostic molecular markers is crucial to understand the underlying mechanisms of LC initiation and progression. The potential role of phosphatidylinositol in tumor growth and the metastatic process has recently been suggested by some researchers. Phosphatidylinositols are lipid molecules and key players in the inositol signaling pathway that have a pivotal role in cell cycle regulation, proliferation, differentiation, membrane trafficking, and gene expression. In this review, we discuss the current understanding of phosphoinositide-specific phospholipase enzymes and their emerging roles in LC.

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Section: Pla and Lung Cancermentioning

confidence: 99%

Phospholipase Family Enzymes in Lung Cancer: Looking for Novel Therapeutic Approaches

Salucci,

Aramini,

Bartoletti-Stella

et al. 2023

Cancers

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“…After adding a protein with phospholipase activity, the lipids will be cleaved, so one of the two acyl chains is cleaved off. This will create a distance between the two fluorescent molecules, and they will start to emit a detectable signal (Figure 12c) (52,53).…”

Section: Fluorescence Based Assaysmentioning

confidence: 99%

Structural and Functional Studies on Evolutionary Repurposing of H-box/NC-proteins : From Host Factor to Virus Protein

Wehlin

2023

Linköping Studies in Science and Technology. Dissertations

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“…The RNAi vector DNA was fixed at gold particles and used for the biolistic bombardment of E. californica wild-type cells by the Bio-Rad PDS-1000/He Particle Delivery System, following a recently published protocol (Heinze and Roos, 2013). The stably transformed cell lines were grown over at least three 9-d passages on selection medium containing 100 mg mL 21 paromomycin and later maintained by growth cycles in medium with 50 mg mL 21 paromomycin.…”

Section: Rnai-based Silencing Of Nhx Genesmentioning

confidence: 99%

The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica

et al. 2015

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Cell cultures of Eschscholzia californica react to a fungal elicitor by the overproduction of antimicrobial benzophenanthridine alkaloids. The signal cascade toward the expression of biosynthetic enzymes includes (1) the activation of phospholipase A2 at the plasma membrane, resulting in a peak of lysophosphatidylcholine, and (2) a subsequent, transient efflux of vacuolar protons, resulting in a peak of cytosolic H + . This study demonstrates that one of the Na + /H + antiporters acting at the tonoplast of E. californica cells mediates this proton flux. Four antiporter-encoding genes were isolated and cloned from complementary DNA (EcNHX1-EcNHX4). RNA interference-based, simultaneous silencing of EcNHX1, EcNHX3, and EcNHX4 resulted in stable cell lines with largely diminished capacities of (1) sodium-dependent efflux of vacuolar protons and (2) elicitor-triggered overproduction of alkaloids. Each of the four EcNHX genes of E. californica reconstituted the lack of Na + -dependent H + efflux in a Dnhx null mutant of Saccharomyces cerevisiae. Only the yeast strain transformed with and expressing the EcNHX1 gene displayed Na + -dependent proton fluxes that were stimulated by lysophosphatidylcholine, thus giving rise to a net efflux of vacuolar H + . This finding was supported by three-dimensional protein homology models that predict a plausible recognition site for lysophosphatidylcholine only in EcNHX1. We conclude that the EcNHX1 antiporter functions in the elicitor-initiated expression of alkaloid biosynthetic genes by recruiting the vacuolar proton pool for the signaling process.

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Assay of Phospholipase A Activity

Cited by 7 publications

References 19 publications

Phospholipase Family Enzymes in Lung Cancer: Looking for Novel Therapeutic Approaches

Phospholipase Family Enzymes in Lung Cancer: Looking for Novel Therapeutic Approaches

Structural and Functional Studies on Evolutionary Repurposing of H-box/NC-proteins : From Host Factor to Virus Protein

The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica

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