Assay of the synthetic estrogen fosfestrol in pharmaceutical formulations using capillary electrophoresis

Themelis, Demetrius G.; Trellopoulos, Anastasios V.; Tzanavaras, Paraskevas D.; Karlberg, Bo

doi:10.1016/j.jpba.2005.05.007

Cited by 8 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to monitoring drug levels in patients quality control of the purity of pharmaceutical formulations is also important. Recently Themelis et al [182] described a validated method to determine the purity of the prodrug, fosfesterol, by CZE. They were able to show that quantization by their method compared favorably with the USP photometric assay raising the possibility of measuring the drug, after removal of plasma proteins, in patients.…”

Section: Cytostaticsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

View full text Add to dashboard Cite

Over the past 15 years, CE as an analytical tool has shown great promise in replacing many conventional clinical laboratory methods, such as electrophoresis and HPLC. CE's appeal was that it was fast, used very small amounts of sample and reagents, was extremely versatile, and was able to separate large and small analytes, whether neutral or charged. Because of this versatility, numerous methods have been developed for analytes that are of clinical interest. Other than molecular diagnostic and forensic laboratories CE has not been able to make a major impact in the United States. In contrast, in Europe and Japan an increasing number of clinical laboratories are using CE. Now that automated multicapillary instruments are commercially available along with cost-effective test kits, CE may yet be accepted as an instrument that will be routinely used in the clinical laboratories. This review will focus on areas where CE has the potential to have the greatest impact on the clinical laboratory. These include analyses of proteins found in serum and urine, hemoglobin (A1c and variants), carbohydrate-deficient transferrin, forensic and therapeutic drug screening, and molecular diagnostics.

show abstract

Section: Cytostaticsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

View full text Add to dashboard Cite

show abstract

“…This method is the most efficient separation technique available for the analysis of both large and small molecules. There are six types of capillary electro separation available but the most commonly used technique seems to be CZE, which is widely used for analyses of various substances, including active pharmaceutical ingredients (either in formulations or biological fluids), drug counter ions . Taking this into account we decided to use this method to detection and separation of compounds with biological activity.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Grodner

Łukaszkiewicz

Kuran

et al. 2014

J of Separation Science

View full text Add to dashboard Cite

The purpose of this study, the direct separation of aminoalkanol derivatives I and II of 1,7-dimethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0(2,6) ]dec-8-ene-3,5,10-trione, which was found in earlier studies as potential anticancer drugs, were performed. Capillary electrophoresis offers the possibility of fast, cheap, and reproducible separations for compounds I and II. In this paper, the simultaneous separation of I and II by capillary zone electrophoresis has been achieved within 8 min by use of 50 mM phosphate buffer of pH 2.5. Analysis of the two compounds in the serum plasma standards was conducted. Limits of detection of I and II by UV absorbance at 200 nm were achieved in the range of 156.3-156.6 ng/mL. The method was validated for linearity, accuracy, precision, limits of detection, and quantification. The calibration equation revealed a good linear relationship (r(2) = 0.998-0.999). Sufficient recovery was observed in the range of 96.3-99.5%. The method showed good reproducibility with intra- and interday precision of 0.97 and 1.76%, respectively. The quantification limits for the compounds were in the range of 477.0-479.8 ng/mL. The proposed method was applied to the analysis of real serum samples.

show abstract

“…Post-translational protein modification plays an important role in every aspect of cellular processes. Deficiency in protein modification is associated with numbers of human diseases (Themelis et al, 2005;Yang et al, 2016). Ubiquitination is the first and the one of the most prevalent post-translational protein modifications described so far.…”

Section: Introductionmentioning

confidence: 99%

Ufl1 deficiency causes skin pigmentation by up-regulation of Endothelin-1

Wang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Ufmylation (UFM1 modification) is a newly identified ubiquitin-like modification system involved in numerous cellular processes. However, the regulatory mechanisms and biological functions of this modification remain mostly unknown. We have recently reported that Ufmylation family genes have frequent somatic copy number alterations in human cancer including melanoma, suggesting involvement of Ufmylation in skin function and disease. UFL1 is the only known Ufmylation E3-like ligase. In this study, we generated the skin-specific Ufl1 knockout mice and show that ablation of Ufl1 caused epidermal thickening, pigmentation and shortened life span. RNA-Seq analysis indicated that Ufl1 deletion resulted in upregulation of the genes involved in melanin biosynthesis. Mechanistically, we found that Endothelin-1 (ET-1) is a novel substrate of Ufmylation and this modification regulates ET-1 stability, and thereby deletion of Ufl1 upregulates the expression and secretion of ET-1, which in turn results in up-regulation of genes in melanin biosynthesis and skin pigmentation. Our findings establish the role of Ufl1 in skin pigmentation through Ufmylation modification of ET-1 and provide opportunities for therapeutic intervention of skin diseases.

show abstract

Assay of the synthetic estrogen fosfestrol in pharmaceutical formulations using capillary electrophoresis

Cited by 8 publications

References 12 publications

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Ufl1 deficiency causes skin pigmentation by up-regulation of Endothelin-1

Contact Info

Product

Resources

About

Assay of the synthetic estrogen fosfestrol in pharmaceutical formulations using capillary electrophoresis

Cited by 8 publications

References 12 publications

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.02,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs

Ufl1 deficiency causes skin pigmentation by up-regulation of Endothelin-1

Contact Info

Product

Resources

About

Capillary electrophoresis separation of aminoalkanol derivatives of 1,7‐dimethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.0^2,6]dec‐8‐ene‐3,5,10‐trione as potential anticancer drugs