Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

Starek, Małgorzata; Guja, Anna; Dąbrowska, Monika; Krzek, Jan

doi:10.1007/s12161-014-0019-0

Cited by 16 publications

(12 citation statements)

References 36 publications

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“…The nutritionists have recommended that the public should eat more vegetables and fruits. Drinking juice is an effective method to promote consumption of fruit and vegetables, and is very popular in many countries [17,18,19,20]. A large amount of evidence shows that juice is a part of balanced diet offering profound risk reduction of many diseases, such as cancers, neurodegenerative diseases, and cardiovascular diseases [18,21,22].…”

Section: Introductionmentioning

confidence: 99%

Effects and Mechanisms of Fruit and Vegetable Juices on Cardiovascular Diseases

Zheng

Zhou

et al. 2017

IJMS

137

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Many studies have indicated that consumption of vegetables and fruits are positively related to lower incidence of several chronic noncommunicable diseases. Although composition of fruit and vegetable juices is different from that of the edible portion of fruits and vegetables, they contain polyphenols and vitamins from fruits and vegetables. Drinking vegetable and fruit juices is very popular in many countries, and also an efficient way to improve consumption of fruits and vegetables. The studies showed that fruit and vegetable juices affect cardiovascular risk factors, such as lowering blood pressure and improving blood lipid profiles. The main mechanisms of action included antioxidant effects, improvement of the aspects of the cardiovascular system, inhibition of platelet aggregation, anti-inflammatory effects, and prevention of hyperhomocysteinemia. Drinking juices might be a potential way to improve cardiovascular health, especially mixtures of juices because they contain a variety of polyphenols, vitamins, and minerals from different fruits and vegetables. This review summarizes recent studies on the effects of fruit and vegetable juices on indicators of cardiovascular disease, and special attention is paid to the mechanisms of action.

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Section: Introductionmentioning

confidence: 99%

Effects and Mechanisms of Fruit and Vegetable Juices on Cardiovascular Diseases

Zheng

Zhou

et al. 2017

IJMS

137

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“…Another HPLC approach's linearity range, accuracy, and precision were reported as 0.10-50 µg mL −1 , 97.50-102.10%, and 1.20-4.40% respectively, all of which were inferior to the greener reversed-phase HPTLC approach [23]. The reported HPTLC approach has a linearity range of 0.76-9.14 µg band −1 , which was significantly inferior than the greener reversed-phase HPTLC approach [31].…”

Section: Comparison With Literature Analytical Approachesmentioning

confidence: 87%

“…Various "high-performance liquid chromatography (HPLC)" approaches are also used to determine β-carotene either alone or in combination with other phytopharmaceuticals in various vegetable crops, nutritional supplements, and commercial formulations [20][21][22][23][24][25][26][27][28]. Several "high-performance thin-layer chromatography (HPTLC)" approaches are also used to determine β-carotene either alone or in combination with other phytopharmaceuticals in various vegetable crops, nutritional supplements, and commercial formulations [29][30][31][32][33][34]. A gas-chromatography mass-spectrometry approach was also applied to identify various carotenoid contents including α-, and β-carotene of minimally processed carrots stored at different temperatures [35].…”

Section: Introductionmentioning

confidence: 99%

A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

Alqarni

Alam

et al. 2021

Agronomy

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Various analytical approaches for determining β-carotene in vegetable crops and commercial dosage forms have been documented. However, neither the qualitative nor quantitative environmental safety and greener aspects of the literature analytical methodologies of β-carotene analysis have been assessed. As a result, the goal of this research is to develop and validate a reversed-phase “high-performance thin-layer chromatography (HPTLC)” approach for determining β-carotene in traditional (TE) and ultrasound-assisted (UBE) extracts of different fractions of Daucus carota (L.), Ipomea batatas (L.), and commercial formulation. The greener mobile phase for β-carotene analysis was a ternary mixture of ethanol, cyclohexane, and ammonia (95:2.5:2.5, v v v−1). The detection of β-carotene was done at a wavelength of 459 nm. In the 25–1000 ng band−1 range, the greener reversed-phase HPTLC approach was linear. Other validation factors for β-carotene analysis, including as accuracy, precision, robustness, and sensitivity, were likewise dependable. The contents of β-carotene were found to be maximum in hexane: acetone (50:50%) fractions of TE and UBE of D. carota and I. batatas compared to their acetone and hexane fractions. The amount of β-carotene in hexane: acetone (50:50%) portions of TE of D. carota, I. batatas and commercial formulation A was estimated to be 10.32, 3.73, and 6.73 percent w w−1, respectively. However, the amount of β-carotene in hexane: acetone (50:50%) portions of UBE of D. carota, I. batatas and commercial formulation A was estimated to be 11.03, 4.43, and 6.89 percent w w−1, respectively. The greenness scale for the proposed HPTLC strategy was calculated as 0.81 using the “analytical GREEnness (AGREE)” method, indicating that the proposed HPTLC methodology has good greenness. The UBE approach for extracting β-carotene outperformed the TE procedure. These results indicated that the greener reversed-phase HPTLC approach can be utilized for the determination of β-carotene in different vegetable crops, plant-based phytopharmaceuticals, and commercial products. In addition, this approach is also safe and sustainable due to the utilization of a greener mobile phase compared to the toxic mobile phases utilized in literature analytical approaches of β-carotene estimation.

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“…Further when compared to standard, the Rf value was determined to be 0.94, on Echinostoma trivolvis rediae isolated from Helisoma trivolvis snails showed the presence of ß-carotenoids with a retention factor (Rf = 0.93) when compared with standard (Fried et al, 1993). Starek and coworkers in 2015 also validated the presence of b -Carotene in Dietary Supplements and Fruit Juices by TLC with a standard b -carotenoid Rf value of 0.99 (Starek et al, 2015).…”

Section: Thin Layer Chromatography Analysismentioning

confidence: 94%

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan

Chokriwal

Stephen

Singh

2019

PRT

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Purpose Carotenoids are pigments that have significant value as colorants and antioxidants in pharmaceutical, food, textile and cosmetic industries. Owing to its high demand, this study aims to identify ß-carotene producing bacteria from different terrestrial habitats of Jaipur region. Design/methodology/approach In this paper, standard isolation and purification process was used, followed by colony morphology and biochemical characterization of ß-carotene producing bacteria. ß-carotene concentration was determined quantitatively using spectrophotometric method. Findings Out of 43 isolates, 21 isolates showed peak range between 400 and 500 nm confirming the presence of carotenoids. Only one bacteria SAN-A has capacity to produce ß-carotene confirmed by the thin layer chromatography and high performance liquid chromatography (HPLC) with a yield of 1.68 mg/l. The 2, 2-Diphenyl-2-picrylhydrazil (DPPH) assay showed an IC50 value of 4.0 mg/ml. Originality/value The present study revealed the presence of ß-carotene producing bacteria in the soil of different terrestrial habitat of Jaipur region which can be exploited as an economical source for ß-carotene production.

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Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

Cited by 16 publications

References 36 publications

Effects and Mechanisms of Fruit and Vegetable Juices on Cardiovascular Diseases

Effects and Mechanisms of Fruit and Vegetable Juices on Cardiovascular Diseases

A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan

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