Assay validation of hair androgens across the menstrual cycle

Wen, Wang; Moody, Shannin N.; Kiesner, Jeff; Appiani, Anna Tonon; Robertson, Olivia; Shirtcliff, Elizabeth A.

doi:10.1016/j.psyneuen.2018.10.029

Cited by 23 publications

(39 citation statements)

References 56 publications

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“…Hair collection. We collected approximately 100 mg of hair near the posterior vertex of the scalp to be shipped at room temperature to the SPIT lab at Iowa State University and then assayed for DHEA, testosterone, and estradiol using the methodology described by (Wang et al, 2019) . We assayed the 5cm closest to the scalp.…”

Section: Methodsmentioning

confidence: 99%

“…Measuring hormone levels in hair from the posterior vertex of the scalp is a more recent development in the puberty literature and has the advantage of being able to derive a cumulative basal hormone level that collapses across extraneous fluctuations. For example, whereas levels of hormones in saliva are affected by diurnal variation and reactivity to acute stressors (Ankarberg & Norjavaara, 1999;Bao et al, 2003;Goodyer et al, 1998;Izawa et al, 2008) , measuring hormones in hair has the advantage of observing presumably more stable, basal measures of hormone over a number of months (Wang et al, 2019) . Measurement of androgens in hair is also reliable in samples of children and adolescents (Smith et al, 2018) .…”

Section: Introductionmentioning

confidence: 99%

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Multi-method confirmatory factor analyses of puberty in early adolescent girls

Byrne¹,

Chavez²,

Vijayakumar³

et al. 2019

Preprint

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(full):Correctly measuring and operationalizing pubertal processes during development is important for capturing variation in both normative development and trajectories associated with adverse outcomes. The aim of the current study was to understand how this variance may be captured by different pubertal processes (adrenarche and gonadarche) and how multiple methods may contribute to variance (and how they are correlated). The study explored multi-method cross-sectional pubertal data, including self-reported physical characteristics from the Pubertal Development Scale (PDS) and Morris & Udry line drawings, and levels of hormones dehydroepiandrosterone (DHEA), testosterone (T), and estradiol (E2) in saliva and hair, from a cohort of 174 early adolescent girls aged 10-13 years. Authors developed a theory-driven structural equation modelling framework of puberty for girls that includes multiple measures of puberty and considers both processes of adrenarche and gonadarche. Self-reported puberty and hormone levels from saliva and hair can estimate an overall pubertal process as well as each process of adrenarche and gonadarche. The self-reported PDS item on height growth had the lowest standardized loading of the self-report items. In full models with all measures, the more parsimonious one-factor model was not a significantly worse fit than the two-factor model. The shared variance between the hormonal latent puberty factor and the self-reported latent puberty factor was 32%. This suggests fairly good reliability between hormones and self-report puberty data but also suggests that measuring puberty via hormones and via self-report questionnaires is not entirely redundant, and models of puberty require collecting both. Exploratory analyses showed 34%, 48%, and 52% shared variance between chronological age and the adrenarcheal, gonadarcheal, and overall pubertal factors, respectively, suggesting that there is additional variance in these pubertal processes that is not explained by age alone. When controlling for age, the adrenarcheal and gonadarcheal factors were still strongly associated (90% shared variance), suggesting that these factors are correlated when calculated as pubertal timing (i.e., stage compared to same-age peers), as well. Finally, correlations with age were higher when using the latent factors, whether for self-report questionnaire data alone, or for hormones, and correlations were highest when using all available self-report and biological measures. Abstract (short):This structural equation modelling study explored how variance in pubertal development is captured by different processes (adrenarche and gonadarche) and methods, using cross-sectional pubertal data from a cohort of 174 early adolescent girls aged 10-13 years. Self-reported puberty and hormone levels from saliva and hair can estimate an overall pubertal process as well as each process of adrenarche and gonadarche. The self-reported PDS item on height growth had the lowest standardized loading of the self-report items. Hormonal and self-reported latent puberty factors had 32% shared variance, suggesting that research should collect both. There was 34%, 48%, and 52% shared variance between chronological age and the adrenarcheal, gonadarcheal, and pubertal factors, respectively, suggesting these processes are not explained by age alone.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multi-method confirmatory factor analyses of puberty in early adolescent girls

Byrne¹,

Chavez²,

Vijayakumar³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Grotzinger (author JGS, 8/2/2018) and that open dialogue led to an erratum which corrected Grotzinger's lower level of detection from 0.1 pg/ml to 0.1 pg/mg (Grotzinger et al, 2018b). Compared to pg/ mg, these hormone concentrations in pg/ml are 6.25 and 25 times higher for DHEA and testosterone, respectively (Wang et al, 2019, by our estimates based on specific hair extraction protocols). Such efficient open dialogue has helped clarify expected concentrations for hair androgens while the literature is still in its nascent stage, not 15 years from now when a typo has become tradition and established the prior art in the field.…”

mentioning

confidence: 78%

“…Decisions of whether to use 200 versus 400 ml of buffer, to incubate for 18–24 hr with or without vibration and/or rotation may be key to establishing the assay's validity, but do not constitute the stroke of ingenuity needed to pass the test of Prior Art . Despite considerable time, money, and thankless effort that goes into testing whether the assay works best with 15 mg or 25 mg of hair, such conclusions should be freely and openly shared with the field (Wang et al, ; Wang, Moody, & Shirtcliff, ).…”

mentioning

confidence: 99%

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Letter to the editor: A call for transparency in immunoassay techniques to enhance rigor and reproducibility

Shirtcliff

Wang

Moody

et al. 2019

Developmental Psychobiology

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Do Parental Hormone Levels Synchronize During the Prenatal and Postpartum Periods? A Systematic Review

Daneshnia,

Chechko,

Nehls

2024

Clin Child Fam Psychol Rev

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Physiological synchrony is the phenomenon of linked physiological processes among two or more individuals. Evidence of linkage between dyads has been found among a broad range of physiological indices, including the endocrine systems. During the transition to parenthood, both men and women undergo hormonal changes that facilitate parenting behavior. The present review sought to address the question as to whether hormonal synchronization occurs among expecting or new parents. A systematic literature search yielded 13 eligible records. The evidence of cortisol synchrony during the prenatal period, with additional testosterone, prolactin, and progesterone covariations in the time leading up to childbirth, was found to be most significant. During the postpartum period, parental synchrony was reported for oxytocin, testosterone, and cortisol levels. The implications of these covariations were found to translate into adaptive parenting behaviors and the facilitation of romantic bond. Associations with infant development were also reported, suggesting far-reaching effects of hormonal synchrony outside the parental dyad. The results highlight the importance of physiological interrelatedness during this sensitive period, underscoring the need for further research in this field. In view of the limited data available in this research domain, we have put forward a framework for future studies, recommending the adoption of standardized research protocols and repeated collections of specimens.

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Assay validation of hair androgens across the menstrual cycle

Cited by 23 publications

References 56 publications

Multi-method confirmatory factor analyses of puberty in early adolescent girls

Multi-method confirmatory factor analyses of puberty in early adolescent girls

Letter to the editor: A call for transparency in immunoassay techniques to enhance rigor and reproducibility

Do Parental Hormone Levels Synchronize During the Prenatal and Postpartum Periods? A Systematic Review

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