2021
DOI: 10.3390/biom11111732
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Assaying Paenibacillus alvei CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release

Abstract: Ketalpyruvyltransferases belong to a widespread but little investigated class of enzymes, which utilise phosphoenolpyruvate (PEP) for the pyruvylation of saccharides. Pyruvylated saccharides play pivotal biological roles, ranging from protein binding to virulence. Limiting factors for the characterisation of ketalpyruvyltransferases are the availability of cognate acceptor substrates and a straightforward enzyme assay. We report on a fast ketalpyruvyltransferase assay based on the colorimetric detection of pho… Show more

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“…Pyruvylation (pyruvate–ketal modification) is a rare modification of glycans located in certain microorganisms. Hager-Mair et al [ 4 ] present a method used to study the enzyme activity of a ketalpyruvyltransferase from Paenibacillus alvei . The new assay is based on the colorimetric detection of phosphate release during pyruvyltransfer from phosphoenolpyruvate onto the acceptor via complexation with Malachite Green and molybdate.…”
mentioning
confidence: 99%
“…Pyruvylation (pyruvate–ketal modification) is a rare modification of glycans located in certain microorganisms. Hager-Mair et al [ 4 ] present a method used to study the enzyme activity of a ketalpyruvyltransferase from Paenibacillus alvei . The new assay is based on the colorimetric detection of phosphate release during pyruvyltransfer from phosphoenolpyruvate onto the acceptor via complexation with Malachite Green and molybdate.…”
mentioning
confidence: 99%