Assembling Two‐Phase Enzymatic Cascade Pathways in Pickering Emulsion

Abstract

The design of enzymatic cascade pathways in biphasic systems with high efficiency is still a big challenge. Herein, we report a simple and efficient strategy for one‐pot biphasic tandem reactions by positioning distinct biocatalysts in spatially separate domains of Pickering emulsion. Due to the large interfacial area and the stability of Pickering interfacial biocatalysts, the as‐obtained Pickering emulsion system was confirmed as a nice microreactor with highly enhanced catalytic performance. Furthermore, in this hybrid catalytic system, unconventional computing relied on enzymatic cascade reactions can be performed. Such Pickering emulsion‐based logic system offers several distinctive advantages, including less “cross‐talk” and signal amplification. We believe that our result will have critical applications in future biocatalysis, nanomachines, smart diagnostics, intelligent drug delivery, nanomechanical, and electronics.

Computing Arithmetic Functions Using Immobilised Enzymatic Reaction Networks

“…Although addition might be straightforward in small networks, designing complex networks with more components is not trivial. The promiscuity of enzyme active sites, substrate competition, and possible side interactions between input or output molecules and the enzymes can significantly influence the final output of the system [25–27] . For example, a substrate saturating the active site of one enzyme can globally affect the conversion of other substrates affecting the final response of the system [22] .…”

“…The promiscuity of enzyme active sites, substrate competition, and possible side interactions between input or output molecules and the enzymes can significantly influence the final output of the system. [25][26][27] For example, a substrate saturating the active site of one enzyme can globally affect the conversion of other substrates affecting the final response of the system. [22] Cross-reactivity can be identified within the overall dynamics of complete experimental sets, as they represent the deviation from the simple model.…”

Logic-gated tumor-microenvironment nanoamplifier enables targeted delivery of CRISPR/Cas9 for multimodal cancer therapy

“…Biocomputing, an ambitious field using biological tools for information processing, displays great potential in sensing systems as well as intelligent machines, which also provides a powerful tool for biomedical implementation including bio-imaging and precision medicine 1 , 2 , 3 , 4 . Recent studies on molecular diagnostics and gene expression profiling have demonstrated remarkable heterogeneity within cancer 5 , 6 .…”