Assembly and Cell Surface Expression of Homomeric and Heteromeric 5-HT3 Receptors: The Role of Oligomerization and Chaperone Proteins

Antidepressants and Antipsychotic Drugs Colocalize with 5-HT3Receptors in Raft-Like Domains

“…Second, we performed immunofluorescence experiments with HA epitope-tagged recombinant 5-HT 3 receptors expressed in HEK 293 cells (HEK-5-HT 3 -HA cells). The epitope tag added between amino acids 5 and 6 of the mature polypeptide appears to be silent (Boyd et al, 2002). The immunofluorescence patterns of DMI-or fluoxetine-treated cells were indistinguishable from those of controls (Fig.…”

“…The human 5-HT 3A receptor subunit cDNA was cloned into the mammalian expression vector pCDM8 (Davies et al, 1999). The tagged 5-HT 3A receptor subunit [5-HT 3A -hemagglutinin (HA)] was obtained by tagging the 5-HT 3A receptor with the HA epitope (YPYDVPDYA) between amino acid 5 and 6 (T29-tag-T30) using sitedirected mutagenesis (Boyd et al, 2002). The mutated cDNA was cloned into the expression vector pRK5.…”

Expression of serotonergic system components during early Xenopus embryogenesis

“…Pharmacological and immunohistochemical data support the possible participation of 5-HT 3 and 5-HT 4 receptors in establishing the left-right asymmetry in Xenopus (Fukumoto et al, 2005b). The 5-HT 3 receptor is a ligand-dependent ionic channel whose functional activity is associated with the presence of HTR3A-subunits (Boyd et al, 2002). Therefore, the absence of HTR3A expression during early embryogenesis results in the absence of de novo formation of the functional 5-HT 3 receptors at this stage.…”

Glutamate Receptor Trafficking: Endoplasmic Reticulum Quality Control Involves Ligand Binding and Receptor Function

“…Oligomeric proteins assembled in the proper conformation escape ER retention by association and masking of their ER retention/retrieval motifs, whereas improper conformations leave these motifs exposed to COPImediated retrieval. Such mechanisms ensuring that only proper oligomeric forms are expressed on the cell surface were shown recently to regulate trafficking of GluR6 KA receptors (S. , NMDA receptors (Okabe et al, 1999;Standley et al, 2000;Scott et al, 2001), nicotinic acetylcholine receptors (Keller et al, 2001), serotonin type-3 receptors (Boyd et al, 2002), K ϩ channels (Zerangue et al, 1999(Zerangue et al, , 2001Hough et al, 2000), Ca 2ϩ channels , and various G-protein-coupled receptors (Margeta-Mitrovic et al, 2000;Calver et al, 2001;Chan et al, 2001;Pagano et al, 2001;Grunewald et al, 2002).…”