Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Abstract

In this paper, a miRNA‐based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR‐27a, miR‐27b, and Let‐7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR‐27a and miR‐27b. The selective sensing of miR‐27a in mixed miRNA solution was also achieved. This miRNA‐based QCM biosensor has the advantages of real‐time, label‐free, and short cycle detection.

Miniaturized differential scanning calorimeter with an integrated mass sensing system: first steps

“…Quartz-crystal microbalances (QCM) change their resonance properties as mass is deposited on the surface of the crystal. The relationship between mass loading of a quartz crystal and a resulting resonant frequency shift was described as early as 1959 (Sauerbrey, 1959); since then, the technique has become increasingly important in research and development (Li et al, 2019;Kadirsoy et al, 2020;Liu et al, 2021). Moreover, resonant cantilevers in the micro-and nanogravimetric range are used for applications in bio/chemical sensing -for example, in combination with optical or piezoresistive detection methods (Ziegler, 2004;Johnson et al, 2012;Li and Lee, 2012;Basu et al, 2020;Jia et al, 2021).…”

Non-Coding RNA-Based Biosensors for Early Detection of Liver Cancer

“…A biosensor based on QCM technology is one of the most popular label-free biosensing platforms for the detection and quantification of a wide range of biomolecules. Furthermore, the high sensitivity and short detection process offered by QCM biosensing assays make them attractive for the development of novel and disposable diagnostic tools [163][164][165]. QCM biosensors consist of a piezoelectric crystal (quartz) coated with a metal electrode and their function is based on the change in frequency of the quartz in response to the adherence of a target molecule.…”