Assembly of newly replicated chromatin

Worcel, Abraham; Han, Stella; Wong, May

doi:10.1016/0092-8674(78)90280-5

Cited by 357 publications

(181 citation statements)

References 32 publications

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“…1) (Jackson 1987(Jackson , 1990Gruss et al 1993). These data are consistent with other experiments demonstrating that incorporation of H3 and H4 precedes H2A and H2B in vivo (Worcel et al 1978) and in vitro (Smith and Stillman 1991). Intriguingly, recent data suggest that histone chaperones may bind dimers of H3/H4 instead of tetramers (Tagami et al 2004) suggesting that two chaperone molecules may act in concert to form new (H3/H4) 2 tetramers.…”

Section: Dna Replicationsupporting

confidence: 87%

“…A variety of data from multiple organisms indicate close coordination between DNA synthesis and histone deposition. Nucleosome formation occurs rapidly following DNA replication in vivo (Worcel et al 1978;Jackson 1990). Additionally, in both human and yeast cells, histone deposition during S phase is required for viability (Han et al 1987;Kim et al 1988;Nelson et al 2002;Nabatiyan and Krude 2004), and in human cells concomitant histone deposition is required for completing DNA replication (Hoek and Stillman 2003;Ye et al 2003).…”

Section: Dna Replicationmentioning

confidence: 99%

“…In vivo, histone deposition and DNA replication are temporally coupled (Worcel et al 1978;Sogo et al 1986;Jackson 1990), and inhibition of either CAF-1 or new histone synthesis in human cells inhibits DNA replication (Nelson et al 2002;Hoek and Stillman 2003;Ye et al 2003). Why does DNA synthesis halt when histone deposition is disrupted?…”

Section: Active Coupling Of Histone Deposition Dna Replicationmentioning

confidence: 99%

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Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Franco¹,

Kaufman²

2004

Cold Spring Harbor Symposia on Quantitative Biology

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Section: Dna Replicationsupporting

confidence: 87%

Section: Dna Replicationmentioning

confidence: 99%

Section: Active Coupling Of Histone Deposition Dna Replicationmentioning

confidence: 99%

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Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Franco¹,

Kaufman²

2004

Cold Spring Harbor Symposia on Quantitative Biology

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“…As the cells returned to G 1 , mono-methylated H4K20 declined. In contrast, the global levels of both di-and trimethylated H4K20 were highest at G 1 /S and slowly declined as cells progressed through S phase, most likely because of the deposition of unmodified histones during DNA replication (38,39). As cells cycled through mitosis, the levels of both steadily increased as they returned to G 1 .…”

Section: Global Changes In H4k20 Methylation Occur During Cell Cyclementioning

confidence: 92%

Catalytic Function of the PR-Set7 Histone H4 Lysine 20 Monomethyltransferase Is Essential for Mitotic Entry and Genomic Stability

Houston

McManus

Adams

et al. 2008

Journal of Biological Chemistry

142

169

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Histone-modifying enzymes play a critical role in modulating chromatin dynamics. In this report we demonstrate that one of these enzymes, PR-Set7, and its corresponding histone modification, the monomethylation of histone H4 lysine 20 (H4K20), display a distinct cell cycle profile in mammalian cells: low at G 1 , increased during late S phase and G 2 , and maximal from prometaphase to anaphase. The lack of PR-Set7 and monomethylated H4K20 resulted in a number of aberrant phenotypes in several different mammalian cell types. These include the inability of cells to progress past G 2 , global chromosome condensation failure, aberrant centrosome amplification, and substantial DNA damage. By employing a catalytically dead dominant negative PR-Set7 mutant, we discovered that its mono-methyltransferase activity was required to prevent these phenotypes. Importantly, we demonstrate that all of the aberrant phenotypes associated with the loss of PR-Set7 enzymatic function occur independently of p53. Collectively, our findings demonstrate that PR-Set7 enzymatic activity is essential for mammalian cell cycle progression and for the maintenance of genomic stability, most likely by monomethylating histone H4K20. Our results predict that alterations of this pathway could result in gross chromosomal aberrations and aneuploidy.Dynamic alterations in chromatin structure are modulated, in part, by the post-translational modifications of the DNAassociated histone proteins. Specialized chromatin-modifying enzymes can phosphorylate, acetylate, ubiquitylate, or methylate specific amino acids within certain histones, and each of these modifications are associated with distinct biological events (1). One of the first histone modifications to be identified nearly forty-five years ago was the methylation of histone H4 lysine 20 (H4K20) 4 (2). Earlier biochemical studies linked H4K20 methylation to diverse biological events including transcriptional regulation, chromatin compaction, cell division, and the formation of heterochromatin (3-9). Importantly, it was also found that H4K20 is differentially methylated in vivo and therefore can be either mono-, di-, or trimethylated (10). Together, these findings strongly suggest that different methylated states of H4K20 may be involved in distinct biological processes, similar to what is observed for the various methylated states of histone H3 lysine 4 and 9 methylation (11, 12).Increasing evidence indicates that certain enzymes are responsible for the specific degree of histone lysine methylation (13). For example, the mono-and dimethylation of histone H3 lysine 9 in humans is mediated by the G9a enzyme, whereas trimethylation is mediated by the SUV39H1 enzyme (14,15). Similarly, the Suv4 -20 enzymes are responsible for di-and trimethylation in mammals (16,17). Trimethylated H4K20 is associated with repressed chromatin because it is targeted to constitutive heterochromatin, various repetitive elements, and imprinting control regions (16,18,19). Dimethylated H4K40 is more widely distributed within...

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“…Histones and transcription factors were found to compete for association with the 5S rRNA gene, yet the first histones to associate with the 5S rRNA genes during chromatin assembly were less repressive toward transcription. Chromatin assembly in vivo and in vitro is optimally coupled to replication (Worcel et al 1978;Almouzni et al 1991;Gruss et al 1990) such that an acetylated histone tetramer (H3/H4)2 is sequestered before H2A/H2B and, finally, HI. This creates a window of opportunity for the programming of the 5S rRNA genes with transcription factors, because the (H3/H4)2 tetramer is less repressive than the complete (H2A/H2B, H3/H4)2 octamer, whereas chromatin containing histone H1 is essentially inert and inaccessible to the class III gene transcriptional machinery.…”

Section: Histone H1 and The Selective Repression Of The Oocyte 5s Rrnmentioning

confidence: 99%

Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

Bouvet

Dimitrov²,

Wolffe³

1994

Genes Dev.

233

154

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The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone HI within chromatin facilitates the activation of the oocyte 5S rRNA genes by TFIIIA. Variation in the amount of histone HI in chromatin does not significantly influence somatic 5S rRNA gene transcription. Thus, the regulated expression of histone HI during Xenopus development has a specific and dominant role in mediating the differential expression of the oocyte and somatic 5S rRNA genes. This example demonstrates that histones can exert dominant repressive effects on the transcription of a gene in vivo in spite of an abundance of transcription factors for that gene.

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Assembly of newly replicated chromatin

Cited by 357 publications

References 32 publications

Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing

Catalytic Function of the PR-Set7 Histone H4 Lysine 20 Monomethyltransferase Is Essential for Mitotic Entry and Genomic Stability

Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

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