2008
DOI: 10.1083/jcb.200804003
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Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3

Abstract: The phosphoinositide phosphatidylinositol 4-phosphate (PtdIns4P) is an essential signaling lipid that regulates secretion and polarization of the actin cytoskeleton. In Saccharomyces cerevisiae, the PtdIns 4-kinase Stt4 catalyzes the synthesis of PtdIns4P at the plasma membrane (PM). In this paper, we identify and characterize two novel regulatory components of the Stt4 kinase complex, Ypp1 and Efr3. The essential gene YPP1 encodes a conserved protein that colocalizes with Stt4 at cortical punctate structures … Show more

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Cited by 146 publications
(206 citation statements)
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“…Recent reports have shown that EFR3 and TTC7 proteins are responsible for the recruitment of PI4KA to the plasma membrane in yeast (Baird et al, 2008) and also in mammalian cells (Nakatsu et al, 2012), a finding we were able to confirm (N.B. and T.B., unpublished observation).…”
Section: Discussionsupporting
confidence: 80%
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“…Recent reports have shown that EFR3 and TTC7 proteins are responsible for the recruitment of PI4KA to the plasma membrane in yeast (Baird et al, 2008) and also in mammalian cells (Nakatsu et al, 2012), a finding we were able to confirm (N.B. and T.B., unpublished observation).…”
Section: Discussionsupporting
confidence: 80%
“…The yeast orthologue of these proteins, Efr3p, was found to be essential for the localization of the yeast PI4K Stt4 into discrete signaling domains and, together with another protein, Ypp1, to control Stt4 function (Baird et al, 2008). A similar role has been described in the mammalian system, where EFR3 and the TTC7 proteins were shown to keep an active pool of PI4KA at the plasma membrane (Nakatsu et al, 2012).…”
Section: Introductionmentioning
confidence: 69%
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“…Intriguingly, two other proteins, Ypp1p (79,1792) and Efr3p, were described as regulators of Stt4p and organizers of the kinase into special signaling domains at contact zones between the ER and the PM (79,980). Ypp1 also targets the Parkinson-related protein, A30P ␣-synuclein to the vacuole for degradation in yeast, again pointing out the possible role of Stt4p at internal membranes.…”
Section: Pi4kiii␣/pi4kamentioning
confidence: 98%
“…Yeast studies showed that its orthologue, Stt4, is important for the production of PM PtdIns(4,5)P 2 (8), and it was also found that the PI4KA enzyme is responsible for maintenance of the PM PtdIns(4,5)P 2 pool in mammalian cells (9,10). The yeast homologue, Stt4, is localized to the PM and organized into signaling domains (11). Although the mammalian PI4KA is primarily located in the endoplasmic reticulum (2,12), recent studies revealed that the EFR3 and TTC7 proteins together recruit the PI4KA enzyme to the PM (13).…”
mentioning
confidence: 99%