Assembly Pathway Selection with DNA Reaction Circuits for Programming Multiple Cell–Cell Interactions

Xiao, Mingshu; Lai, Wei; Yu, Huizhen; Yu, Zijing; Li, Li; Fan, Chunhai; Pei, Hao

doi:10.1021/jacs.0c12358

Cited by 66 publications

(55 citation statements)

References 37 publications

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“…[26] Both DNAzyme and its substrate were modified with cholesterol tail at the 5'-end, so the chains can be inserted into the membranes by hydrophobic interactions. [27] They used Zn 2 + -specific and Mg 2 + -specific RNAcleaving DNAzyme and their substrate strands to trigger the disassembly of cells, which also realized a two-factor controlled disassembly switch. From the 5'-end to the 3'-end, a Zn 2 + -specific DNAzyme is firstly added on AND chain-1, and next is a substrate cleavable by a Mg 2 + -specific DNAzyme.…”

Section: Non-specific Modification Methodsmentioning

confidence: 99%

Recent Progress and Perspectives on Cell Surface Modification

Zhang

Zhou

Qian

2021

Chemistry An Asian Journal

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The cell membrane is a biological interface consisting of phospholipid bilayer, saccharides and proteins that maintains a stable metabolic intracellular environment as well as regulating and controlling the exchange of substances inside and outside the cell. Cell membranes provide a highly complex biological surface carrying a variety of essential surfaces ligands and receptors for cells to receive various stimuli of external signals, thereby inducing corresponding cell responses regulating the life activities of the cell. These surface receptors can be manipulated via cell surface modification to regulate cellular functions and behaviors Thus, cell surface modification has attracted considerable attention due to its significance in cell fate control, cell engineering and cell therapy. In this minireview, we describe the recent developments and advances of cell surface modification, and summarize the main modification methods with corresponding functions and applications. Finally, the prospect for the future development of the modification of the living cell membrane is discussed.

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Section: Non-specific Modification Methodsmentioning

confidence: 99%

Recent Progress and Perspectives on Cell Surface Modification

Zhang

Zhou

Qian

2021

Chemistry An Asian Journal

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“…Taking advantages of the lipid molecules such as stearic acid, cholesterol and palmitic acid, functionalized probes can be inserted into the cell membrane through hydrophobic interaction. [28][29][30][31][32][33] Klymchenko et al synthesized a series of fluorescent probes through the click reaction between alkyl chains and various cyanine fluorophores for fast and uniform imaging of cell membrane. 34 Moreover, lipids and proteins of the cell membrane also give the cell membrane a surplus of negative charge.…”

Section: Introductionmentioning

confidence: 99%

Peptide-Conjugated Aggregation-Induced Emission Fluorogen: Precise and Firm Cell Membrane Labeling by Multiple Weak Interactions

Yang¹,

Hu²,

Wei³

et al. 2022

CCS Chem

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Cell membrane is a vital barrier to protect the cell from outside damage and involved in many biochemical processes. It is of great significance to label the cell membrane to explore its function. However, due to the complex and dynamic nature of cell membrane, precisely and firmly labeling the cell membrane simultaneously is still a challenge. Herein, we report a peptide-conjugated aggregation-induced emission fluorogen (AIEgen), named RTP. RTP mainly consists of three components. (1) RGD peptide (R), which can specifically bind to integrin  v  3 on cell membrane through ligand-receptor interaction. ( 2) T-MY (T), an AIE-active tetraphenylethene derivative for fluorescent imaging. (3) Palmitic acid modified peptide Pal-RRRR (P), in which Pal is inserted into the lipid on the cell membrane by hydrophobic interaction, and RRRR is combined with the negatively charged cell membrane through electrostatic interaction. RTP can precisely label the tumor cells with high integrin  v  3 expression, firmly trace the cell membrane for up to 4 hours, and has strong resistance to photobleaching. Moreover, RTP achieves tumor specific imaging by labeling cell membrane in vivo. Utilizing multiple weak interactions between the fluorescent probe and cell membrane provides a new strategy for precise and firm imaging of cell membrane simultaneously.

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“…Among these materials, amphiphilic DNA has been intensively applied for cell surface engineering and cell–cell interaction regulations [22–27, 30–32] due to their good biocompatibility, high programmability and low cost [33–35] . DNA‐based materials can be easily immobilized on the membrane surface by hydrophobic insertion of hydrophobic group labelled on DNA strand, which avoids complex genetic manipulation, toxic chemical reactions, time‐consuming metabolic regulation and other problems.…”

Section: Introductionmentioning

confidence: 99%

Manipulation of Multiple Cell–Cell Interactions by Tunable DNA Scaffold Networks

et al. 2021

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Manipulation of cell-cell interactions via cell surface engineering has potential biomedical applications in tissue engineering and cell therapy. However, manipulation of the comprehensive and multiple intercellular interactions remains a challenge and missing elements. Herein, utilizing a DNA triangular prism (TP) and a branched polymer (BP) as functional modules, we fabricate tunable DNA scaffold networks on the cell surface. The responsiveness of cell-cell recognition, aggregation and dissociation could be modulated by aptamer-functionalized DNA scaffold networks with high accuracy and specificity. By regulating the DNA scaffold networks coated on the cell surface, controlled intercellular molecular transportation is achieved. Our tunable network provides a simple and extendible strategy which addresses a current need in cell surface engineering to precisely manipulate cell-cell interactions and shows promise as a general tool for controllable cell behavior.

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Assembly Pathway Selection with DNA Reaction Circuits for Programming Multiple Cell–Cell Interactions

Cited by 66 publications

References 37 publications

Recent Progress and Perspectives on Cell Surface Modification

Recent Progress and Perspectives on Cell Surface Modification

Peptide-Conjugated Aggregation-Induced Emission Fluorogen: Precise and Firm Cell Membrane Labeling by Multiple Weak Interactions

Manipulation of Multiple Cell–Cell Interactions by Tunable DNA Scaffold Networks

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