Abstract. This paper has improved the traditional interference matrix, with joining the information of robotic assembly resource, which is used to search for the optimal assembly sequence. We have given a processing method of interference matrix containing the resource of the robotic assembly, which includes the decision method of geometry feasibility, method of judging the interference between two parts. By dealing with the proposed interference matrix, we define a fitness function to evaluate the value of the various assembly sequences. At last, the paper use Discrete Particle Swarm Optimization (DPSO) algorithm to search the optimal assembly sequence, which is more conformable to actually assemble by using robot.