This paper analyses the phytochemical composition, and antioxidant activity of new selected biotypes of cherry silverberry grown in Poland. In addition, the polyphenolic contents, sugars, and vitamin C, as well as organic acid profile were evaluated. Among the researched compounds, two sugars, seven organic acids, and six fatty acids were reported in new studied biotypes of cherry silverberry. Some new biotypes presented a high contents of vitamin C, linoleic, α-linoleic acids (si1, and si2), glucose, fructose, ash (si5, and si4), other fatty acids, organic acids, and antioxidant activity (si0, and si3). They displayed an average contents of polyphenols [5.68 g GAE/100 g dry matter (d.m.)], vitamin C (15.13 mg/100 g), ash (0.56%), fatty acids (89.99%), organic acids (22.41 g/100 g d.m.), sugars (47.96 g/100 g d.m.), and high antioxidant activity of 3.62 mM Trolox (TE)/100 g d.m. (FRAP assay), and 5.11 mM TE/100 g d.m. (DPPH assay). The new biotypes of cherry silverberry cultivated in Poland might be a good source for the production of functional foods, and direct consumption. Moreover, cherry silverberry is an inquisitive material with a different phytochemical components, and according to the principal component analysis, strongly dependent on the biotype.