Assesment of the Resistance of Clinical Isolates Pseudomonas Aeruginosa to Quinolonones

Savov, Encho; Trifonova, Angelina; Todorova, Irina; Gergova, Ivanka; Borisova, M.; Ananieva, M. M.; Kjoseva, E.; Kardjeva, None Velichka

doi:10.15547//tjs.2014.03.001

Cited by 2 publications

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“…The mechanism of action of ciprofloxacin is inhibition of DNA gyrase and topoisomerase IV. DNA gyrase is the bacterial topoisomerase II that controls the topology of the double helix of DNA during the replication and translation process (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

The role of Gene Mutations (gyrA, parC) in Resistance to Ciprofloxacin in Clinical Isolates of Pseudomonas Aeruginosa

et al. 2021

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Scan to discover onlineBackground & Objective: Pseudomonas aeruginosa is an opportunistic pathogen and one of the most common causes of nosocomial infections. This bacterium's antibiotic resistance to the common fluoroquinolone antibiotics, especially ciprofloxacin, is due to mutations in the gyrA and parC genes. This study aimed to investigate the effect of the mutation in (gyrA, parC) on ciprofloxacin resistance in clinical isolates of Pseudomonas aeruginosa.Methods: A total of 140 clinical samples were collected from hospitals. The samples were identified by standard biochemical tests, and the antibiotic resistance was investigated by the disk diffusion method. DNA was extracted from 30 isolates, and PCR was performed. PCRsequencing was carried out to assess gyrA and parC mutations in drug-resistant isolates. NCBI-Blast and MEGA7 software was used to analyze the nucleotide sequences.Results: 30 clinical isolates were 80% resistant to ciprofloxacin; meanwhile, in 21 samples, mutations were observed. 87/5% of mutations were related to gyrA (Thr83 → Ile), 79/16 % parC (Ser87 → Leu), and 4/18% (Glu91 → Lys). The antibiotic resistance to ciprofloxacin and mutations in gyrA and parC genes in resistant isolates are significantly related to each other (P<0.05). Conclusion:The mutations in the gyrA and parC genes play an essential role in resistance to ciprofloxacin in clinical isolates of Pseudomonas aeruginosa.

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Section: Discussionmentioning

confidence: 99%