Assessing Activity of Antimicrobial Agents and Screening Antibiotic-Resistant Bacteria Through DREAM Assay

A low-cost optofluidic platform for the colorimetric assessment of bacterial activity in domestic wastewater

“…The volumetric ratio of the bacterial culture and methylene blue in the previous studies 22–25 was 400 : 1, i.e. , 2 mL of bacterial culture mixed with 5 μL of methylene blue at a 50 mM concentration.…”

“…Optimum concentration of methylene blue. The volumetric ratio of the bacterial culture and methylene blue in the previous studies [22][23][24][25] was 400 : 1, i.e., 2 mL of bacterial culture mixed with 5 mL of methylene blue at a 50 mM concentration. This is because the assay was performed in a cuvette.…”

“…This assay has been used for assessing the activity of antimicrobial agents and screening antibiotic-resistant bacteria. 23 This assay has also been used to assess the microbial electron-transfer activity in inocula 24 and screening sediment samples used as analytes in microbial fuel cells. 25…”

“…This assay has been used for assessing the activity of antimicrobial agents and screening antibiotic-resistant bacteria. 23 This assay has also been used to assess the microbial electrontransfer activity in inocula 24 and screening sediment samples used as analytes in microbial fuel cells. 25 Bacterial activity assessment of untreated wastewater is primarily important for determining the optimal treatment method and dening treatment parameters that are best suited for decentralized wastewater treatment setups.…”

A selection strategy for enhancing exoelectrogenic consortium towards improved power generation in microbial fuel cells

“…It must be noted that reduction of methylene is performed only by live cells and not by dead cells, medium components or metabolites. 19 This assay can be utilised in a cost-effective and rapid manner to study electron transfer activity of microbes. The Fe-reducing cultures that were responsible for increased methylene blue decolourization performed reduction in a manner similar to dissimilatory metal-reducing microbes.…”