Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar (Populus spp.)

Zhu, Wenxu; Chu, Ying‐Hao; Ding, Changjun; Huang, Qinjun; Zhang, Bingyu; Zhang, Weixi; Su, Xiaohua

doi:10.1007/s11676-015-0184-z

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…These results indicated that expression of the AhDREB1 gene in Populus does not affect the number of culturable microorganisms present. Similar results were also shown by Zhu et al [35]; in their study, there was no or very limited impact of an eight-year-old transgenic poplar on the bacterial communities in the rhizosphere. However, more than 90% of microorganisms are not culturable [36].…”

Section: Discussionsupporting

confidence: 90%

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

Guo

Luo

et al. 2018

Forests

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Populus tomentosa grow rapidly, but are salt susceptible. To quickly and efficiently gain new poplar breeds with better salt resistance, a DREB transcription factor derived from Atriplex hortensis was transformed into triploid Populus tomentosa by our lab, which significantly improved the salt tolerance of host plants. However, environmental impacts of transgenic plants must be assessed before large-scale cultivation in China. Here, we conducted a field trial of AhDREB1 transgenic and non-transgenic triploid Populus tomentosa to assess the impact of transgenic trees on rhizospheric soil microbial communities and allelopathic activity of leaves. No significant differences in the number of soil microbes present were detected between the transgenic lines and the non-transgenic controls. The allelopathic activity of leaves from both the transgenic and non-transgenic lines varied with sampling time, but did not differ significantly between the transgenic and non-transgenic lines. These results indicate that the impact on the environment of AhDREB1 transgenic P. tomentosa did not differ significantly from that of the non-transformed controls for the variables observed in this field trial. We also investigated the persistence of AhDREB1 genes in decomposing transgenic poplar leaf on the soil under natural conditions for five months, and our data indicated that fragments of the genetically modified DNA were not detectable in the field after more than two months. We used a triphenyl tetrazolium chloride test (TTC) (or pollen germination method) and hybridization to test the pollen viability and fertility, respectively, of the transgenic and non-transgenic trees and the results showed that the pollen viability of both the transgenic and non-transgenic trees was extremely low in 2016; the receptor plant may have been sterile.

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Section: Discussionsupporting

confidence: 90%

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

Guo

Luo

et al. 2018

Forests

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“…Transgenic tobacco also had altered rhizosphere/rhizoplane microbial communities; however, these effects were temporary, and the diversity of the community was restored to the original level after one cycle of plant cultivation (Andreote et al, 2008). Previous research in our laboratory found no significant differences in bacterial communities between rhizosphere soils of 8-year-old GM and non-GM poplars ( Populus × euramericana ‘ Guariento ’) (Zhu et al, 2016). Our transgenic poplar is different from most transgenic plants in that the exogenous gene, the JERF transcription factor, does not contain the Bt gene, and does not release the BT protein into the plant body and surrounding soil.…”

Section: Discussionmentioning

confidence: 53%

“…However, to date, studies on the effects of transgenic plants on the rhizosphere and endogenous microorganisms have mainly focused on annual crops or herbaceous plants, while there are few reports on trees with long growth cycles. Previous research in our laboratory found no significant differences in bacterial communities between rhizosphere soils of 8-year-old genetically modified (GM) and non-GM poplar ( Populus × euramericana ‘Guariento’) (Zhu et al, 2016).…”

Section: Introductionmentioning

confidence: 67%

Endophytic Communities of Transgenic Poplar Were Determined by the Environment and Niche Rather Than by Transgenic Events

et al. 2019

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Microbial communities associated with plants represent key determinants of plant health, survival, and growth. However, a good understanding of the structural composition of the bacterial and fungal microbiome present in different plant tissues and growing environments, especially in transgenic woody plants, is required. In the present study, we hypothesized that environmental conditions, ecological niches, and transgenic events could influence the community structure of plant-associated microorganisms (bacterial and fungal endophytes). We sampled the root and stem endospheres of field-grown transgenic and non-transgenic poplar trees ( Populus alba × P. berolinensis ) and applied 16S rRNA and internal transcribed spacer amplicon Illumina MiSeq sequencing to determine the bacterial and fungal communities associated with the different plant habitats and tissues. We found that actinobacteria, proteobacteria, bacteroidetes, and firmicutes were the dominant endophytic bacteria, and the fungal community was dominated by dothideomycetes, agaricomycetes, leotiomycetes, and sordariomycetes. In conclusion, transgenic events did not affect the endophytic bacterial and fungal diversity of poplar trees. The bacterial and fungal community structure depends on the pH and the soil organic matter content. Each plant tissue represents a unique ecological niche for the microbial communities. Finally, we identified the indicator operational taxonomic units (OTUs) and core microbiome associated with the different plant tissues of Populus and different environmental conditions. The results provide a basis for further study of host-microbial interactions with the identified abundant OTUs of Populus .

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“…In forestry, transgenic genetic poplar, such as insect resistant transgenic poplar, has been approved and become possibility of deployment ( Jing et al, 2004 ; Guifeng et al, 2006 ; Guifeng et al, 2006 ; Zhou et al, 2018 ). More transgenic poplars are still in the stage of trial and environmental risk assessment ( Lu et al, 2014 ; Zhu et al, 2016 ; Hu et al, 2017 ). Five years of field monitoring of the Bt transgenic 741 poplar showed that the introduction of the Bt gene has neither affected the stability of arthropod communities nor the rhizosphere soil physicochemical properties and microbial community structure ( Zuo et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil

Zheng

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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Rhizosphere microorganisms are essential parts in maintaining soil ecological functions. Reforestation using genetically modified trees might have great potential to enhance tree production in biotic and abiotic stress, however, their long-term impact on rhizosphere microorganisms is scant. In this study, we studied soil enzyme activities and composition of rhizosphere microorganisms in 2-year-old transgenic PaGLK overexpression (OE), repressed expression (RE) and wild-type (WT) poplar (P. alba × P.berlinensis). The root exudates of PaGLK transgenic poplar (P.alba × P. berlinensis) were analyzed by liquid chromatography-mass spectrometry (LC-MS). The results showed that there were significant difference for soil sucrase, urease, catalase, neutral protease and cellulase between the transgenic and WT lines at different growth periods. Alpha diversity analysis showed that bacterial community abundance and diversity for RE lines were significantly lower than WT (p < 0.05), while RE lines for fungi were significantly higher than WT lines. At the genus level, Burkholderia was the dominant group of rhizosphere bacterial community, and the relative abundance for RE was significantly higher than WT. Tomentella was the dominant group for fungi community. Serendipita for RE was significantly higher than WT and OE. Main metabolite contents of (S)-ACPA, geniposidic acid, agnuside, hydroquinone and pyranocoumarins were significantly different among transgenic lines. These results suggest that transgenic activities have effects on root exudates, rhizosphere soil enzyme activities and soil microbial community composition, but long term effects need to be further investigated.

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Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar (Populus spp.)

Cited by 5 publications

References 49 publications

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

Endophytic Communities of Transgenic Poplar Were Determined by the Environment and Niche Rather Than by Transgenic Events

Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil

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