- Assessing Bacterial Diversity in a Seawater-Processing Wastewater Treatment Plant by 454-Pyrosequencing of the 16S rRNA and amoA Genes

doi:10.1201/b18618-11

Cited by 5 publications

(8 citation statements)

References 28 publications

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“…As expected, decreased bacterial diversity was observed in the MF and RO biofilms compared with the secondary effluent; i.e., the influent to the AWPF composed of activated sludge and trickling filter effluent (Q1). It is well understood that proliferation of microorganisms during the activated sludge treatment process will increase microbial activity, thereby enabling removal of organics, toxins, and nutrients (Wang et al 2014). As was observed in the current study, undisinfected Q1 influent would be expected to have greater bacterial diversity than MF and RO biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…Further screening and method assessments are needed to ensure detection and identification of all fungi present, in particular for those present in low abundance (Tan et al 2015). Previous studies using molecular techniques have identified fungi DNA in WWTPs (Kwiatkowska & Zielinska 2016, Wang et al 2014), but the current study is the first to characterize fungi DNA that is present in biofilms related to MF and RO membranes.…”

Section: Figure 5 Relative Abundance Of Bacteria Genera In Mf and Ro mentioning

confidence: 96%

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Characterization of Microbial Signatures From Advanced Treated Wastewater Biofilms

et al. 2017

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Next‐generation sequencing (NGS) and metagenomics were used to identify microbial communities in biofilms of microfiltration (MF) and reverse osmosis (RO) membranes in an advanced water purification facility (AWPF) that treats municipal wastewater to produce potable quality water. Secondary treated wastewater effluent is the source of influent to the AWPF treatment train and was also characterized by NGS. Results show low bacterial diversity in biofilms obtained from the feed‐side surfaces of MF and RO membranes. Microbial communities and antibiotic resistance genes (ARGs) in the RO biofilm were compared with those of the MF and influent, revealing lower abundance of bacterial species and ARGs in the RO biofilm than in the other samples. Opportunistic pathogens were detected in all samples; however, indicator bacteria, viruses, and bacteriophages were not detected in the RO biofilm. It is concluded that NGS has great potential for improved detection and characterization of microbial communities in biofilms that form on AWPF MF and RO membranes.

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Section: Discussionmentioning

confidence: 99%

Section: Figure 5 Relative Abundance Of Bacteria Genera In Mf and Ro mentioning

confidence: 96%

Characterization of Microbial Signatures From Advanced Treated Wastewater Biofilms

et al. 2017

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“…Recently, GeoChip 3.0 and the GeoChip 4 series have been applied to the investigations of industrial WWTPs (Zhang et al 2013) and municipal wastewater treatment systems (Sun et al 2014;Wang et al 2014), respectively. The studies successfully revealed some linkages between functional potential and environmental parameters.…”

Section: Introductionmentioning

confidence: 99%

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

Xia

Wang

Wen

et al. 2014

Appl Microbiol Biotechnol

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Understanding microbial community composition is thought to be crucial for improving process functioning and stabilities of full-scale activated sludge reactors in wastewater treatment plants (WWTPs). However, functional gene compositions of microbial communities within them have not been clearly elucidated. To gain a complete picture of microbial community, in this study, GeoChip 4.2 was used to profile the overall functional genes of three full-scale activated sludge bioreactors, the 16S rRNA gene diversities of which had been unveiled by 454-pyrosequencing in our previous investigation. Triplicate activated sludge samples from each system were analyzed, with the detection of 38,507 to 40,654 functional genes. A high similarity of 77.3-81.2 % shared functional genes was noted among the nine samples, verified by the high 16S rRNA gene similarity with shared operational taxonomic units (OTUs) constituting 66.4-70.0 % of the detected sequences in each system. Correlation analyses showed that the abundances of a wide array of functional genes were associated with system performances. For example, the abundances of carbon degradation genes were strongly correlated to chemical oxygen demand (COD) removal efficiencies (r=0.8697, P<0.01). Lastly, we found that sludge retention time (SRT), influent total nitrogen concentrations (TN inf), and dissolved oxygen (DO) concentrations were key environmental factors shaping the overall functional genes. Together, the results revealed vast functional gene diversity and some links between the functional gene compositions and microbe-mediated processes.

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“…However, bacterial community structures of aerobic sludge (A-O) and anaerobic sludge (A-A) of WWTPA were clearly separated, both of which were dramatically different from those of WWTPB. The difference in wastewater quality may result in the divergence of the aerobic sludge communities of the two WWTPs, and available oxygen is considered a crucial factor contributing to the difference between the aerobic and anaerobic sludge samples [25,26].…”

Section: Biodiversity and Functions Of Microbial Communities Of The Wmentioning

confidence: 99%

Abundance and Diversity of Bacterial Nitrifiers and Denitrifiers and Their Functional Genes in Tannery Wastewater Treatment Plants Revealed by High-Throughput Sequencing

WANG¹,

ZHANG²,

Lu³

et al. 2015

Bioremediation of Wastewater

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Biological nitrification/denitrification is frequently used to remove nitrogen from tannery wastewater containing high concentrations of ammonia. However, information is limited about the bacterial nitrifiers and denitrifiers and their functional genes in tannery wastewater treatment plants (WWTPs) due to the low-throughput of the previously used methods. In this study, 454 pyrosequencing and Illumina high-throughput sequencing, combined with molecular methods, were used to comprehensively characterize structures and functions of nitrification and denitrification bacterial communities in aerobic and anaerobic sludge of two fullscale tannery WWTPs. Pyrosequencing of 16S rRNA genes showed that Proteobacteria and Synergistetes dominated in the aerobic and anaerobic sludge, respectively. Ammonia-oxidizing bacteria (AOB) amoA gene cloning revealed that Nitrosomonas europaea dominated the ammonia-oxidizing community in the WWTPs. Metagenomic analysis showed that the denitrifiers mainly included the genera of Thauera, Paracoccus, Hyphomicrobium, Comamonas and Azoarcus, which may greatly contribute to the nitrogen removal in the two WWTPs. It is interesting that AOB and ammonia-oxidizing archaea had low abundance although both WWTPs demonstrated high ammonium removal efficiency. Good correlation between the qPCR and metagenomic analysis is observed for the quantification of functional genes amoA, nirK, nirS and nosZ, indicating that the metagenomic approach may be a promising method used to comprehensively investigate the abundance of functional genes of nitrifiers and denitrifiers in the environment.

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- Assessing Bacterial Diversity in a Seawater-Processing Wastewater Treatment Plant by 454-Pyrosequencing of the 16S rRNA and amoA Genes

Cited by 5 publications

References 28 publications

Characterization of Microbial Signatures From Advanced Treated Wastewater Biofilms

Characterization of Microbial Signatures From Advanced Treated Wastewater Biofilms

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

Abundance and Diversity of Bacterial Nitrifiers and Denitrifiers and Their Functional Genes in Tannery Wastewater Treatment Plants Revealed by High-Throughput Sequencing

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