Assessing biodegradation of furfuryl alcohol using Pseudomonas putida MTCC 1194 and Pseudomonas aeruginosa MTCC 1034 and its kinetics

Abstract

The biodegradation of furfuryl alcohol (FA) in shake flask experiments using a pure culture of Pseudomonas putida (MTCC 1194) and Pseudomonas aeruginosa (MTCC 1034) was studied at 30 °C and pH 7.0. Experiments were performed at different FA concentrations ranging from 50 to 500 mg/l. Before carrying out the biodegradation studies, the bacterial strains were acclimatized to the concentration of 500 mg/l of FA by gradually raising 100 mg/l of FA in each step. The well acclimatized culture of P. putida and P. aeruginosa degraded about 80 and 66% of 50 mg/l FA, respectively. At higher concentration of FA, the percentage of FA degradation decreased. The purpose of this study was to determine the kinetics of biodegradation of FA by measuring biomass growth rates and concentration of FA as a function of time. Substrate inhibition was calculated from experimental growth parameters using the Haldane equation. Data for P. putida were determined as µ = 0.23 h, K = 23.93 mg/l and K = 217.1 mg/l and for P. aeruginosa were determined as µ = 0.13 h, K = 21.3 mg/l and K = 284.9 mg/l. The experimental data were fitted in Haldane, Aiba and Edwards inhibition models.

High biorenewable content acrylate photocurable resins for DLP 3D printing

“…Glycerol ester groups and furan ring in polymers AESO/THFA and AESO/THFMA were triggered by microorganisms and participated in biodegradation process. Furan ring moieties cleave in the place of oxygen heteroatom forming various levulinic acid or 2‐furoic acid derivatives, which further cleave into smaller molecules 64,65 . Due to this degradation mechanism, all furan derivatives are biodegradable, although the rate of biodegradation is irregular as it was observed for polymers AESO/THFA and AESO/THFMA.…”

“…Furan ring moieties cleave in the place of oxygen heteroatom forming various levulinic acid or 2-furoic acid derivatives, which further cleave into smaller molecules. 64,65 Due to this degradation mechanism, all furan derivatives are biodegradable, although the rate of biodegradation is irregular as it was observed for polymers AESO/THFA and AESO/THFMA. The rate of biodegradation of these polymers differs due to the different density of cross-links.…”

Isolation of Furfuryl Alcohol Degrading Bacteria from Aromatic Compound‐Based Industrial Waste

“…[ 4 ] In addition, bacterial cell mass was monitored by taking optical density at 600 nm at the interval of 24 h for 7 days. [ 5 ] Figures and show the growth curve of isolates from soil samples collected from sites A, B, and C.…”

Degradation of Polyfurfuryl Alcohol-based Biopolymer by Soil-burial and Photo-degradation Methods

“…The most alluring aspect of PFA-based biopolymers is the capacity of FA to degrade by micro-organisms in general and enzymes in particular [9]. To assess the potential of any biopolymer as commodity materials, it is important to study it's degradation aspect in environmental condition [10].…”