Assessing detoxification of a complex hazardous waste, using the Microtox?bioassay

Symons, Brian D.; Sims, Ronald C.

doi:10.1007/bf01055515

Cited by 51 publications

(26 citation statements)

References 6 publications

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“…One of the main factors for determining toxicity in a Microtox assay seems to be compound solubility or bioavailability. The Microtox assay is a microbial assay used for assessing the toxicity of complex wastes, the effectiveness of treatment of hazardous wastes, and it has recently extended to assessment of treatability of hazardous organic compounds in soil (Symons and Sims 1988). However, no single bioassay procedure can provide a comprehensive toxicity evaluation of a complex mixture such as oil.…”

Section: Resultsmentioning

confidence: 99%

Determination of Genotoxicity Using a Chloroperoxidase-Mediated Model of PAH-DNA Adduct Formation

Márquez-Rocha

Pica-Granados

Sandoval-Villasana

et al. 1997

Bulletin of Environmental Contamination and Toxicology

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Section: Resultsmentioning

confidence: 99%

Determination of Genotoxicity Using a Chloroperoxidase-Mediated Model of PAH-DNA Adduct Formation

Márquez-Rocha

Pica-Granados

Sandoval-Villasana

et al. 1997

Bulletin of Environmental Contamination and Toxicology

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“…Early environmental applications of biosensors employed naturally luminescent (490 nm) bacteria such as Vibrio fischeri (re-classified as Aliivibrio fischeri) for monitoring water quality [18], sediments and pore water toxicity [17,31], waste toxicity [122] and efficiency of bioremedation [137] [18,46,52,60,69,129]. These constitutive naturally luminescent biosensors provide information on the soil and environmental toxicity because the luciferase activity is related to cell integrity and full metabolic activity and is reduced upon cell damage or toxicity.…”

Section: Applications Of Biosensors To Soil and Environmental Studiesmentioning

confidence: 99%

Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

Renella

2016

Chem. Biol. Technol. Agric.

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Whole-cell biosensors are natural or engineered microorganisms producing signals in response to specific stimuli. This review introduces the use of whole-cell biosensors for the study of the soil system, discuss the recent developments and some current limitations and draws future prospects of the whole-cell biosensors for application to the study of the agro-ecosystems. The review focuses mainly on the lux-and gfp-inserted whole-cell biosensors producing bioluminescence and multicoloured fluorescent proteins, which allow an easy and reproducible detection of the signals from a large number of prokaryotic and eukaryotic soil-borne microorganisms. This review also points out how the whole-cell biosensors indicate the bioavailability of selected analyte, an information that cannot be straight forwardly extrapolated using the chemical methods of soil analysis. However, regardless of the immense progress in biotechnology and genetics that allows to construct whole-cell biosensors for virtually detecting any chemical at ultra low concentrations, the soil still remains the most extreme natural system to be studied with these biotechnological analytical tools. Although a lack of standardization for most of the constructed whole-cell biosensors along with the scarce knowledge of their performance concur to prevent their use in the official methods of soil and environmental analysis, owing to their stability and selectivity we restate that the whole-cell biosensors are ready to provide information on the main processes occurring in soil, and represent unprecedented sensitive tools for improving agriculture and for soil monitoring.

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“…Mixtures then were left for 6 hrs for sedimentation of soil particles. 22 % NaCl solution was added to the supernatant to obtain the final concentration of 2% NaCl (Symons & Sims, 1988;Skacel et al, 1993;Demuth et al, 1993).…”

Section: Ecotoxicity Assessmentmentioning

confidence: 99%

US DOE and Polish Institute for Ecology of Industrial Areas JCCES FY01 Annual Report

Altman¹

2002

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Assessing detoxification of a complex hazardous waste, using the Microtox?bioassay

Cited by 51 publications

References 6 publications

Determination of Genotoxicity Using a Chloroperoxidase-Mediated Model of PAH-DNA Adduct Formation

Determination of Genotoxicity Using a Chloroperoxidase-Mediated Model of PAH-DNA Adduct Formation

Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

US DOE and Polish Institute for Ecology of Industrial Areas JCCES FY01 Annual Report

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