Assessing Differences between Clinical Isolates of Aspergillus fumigatus from Cases of Proven Invasive Aspergillosis and Colonizing Isolates with Respect to Phenotype (Virulence in Tenebrio molitor Larvae) and Genotype

El-Kamand, Sam; Steiner, Martina; Ramirez, Carl; Halliday, Catriona; Chen, Sharon C‐A; Papanicolaou, Alexie; Morton, Oliver

doi:10.3390/pathogens11040428

Cited by 2 publications

(1 citation statement)

References 76 publications

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“…Comparative studies involving clinical isolates of A. fumigatus demonstrated decreased survival rates in T. molitor larvae with increasing fungal inoculum concentrations. Lower inoculum concentrations corresponded to reduced fungal burden, suggesting fungus shedding despite heightened larval mortality [ 210 ]. Similarly, the virulence of different species of Malassezia was evaluated in the T. molitor model.…”

Section: The Tenebrio Molitor Modelmentioning

confidence: 99%

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Fusco-Almeida,

de Matos Silva,

dos Santos

et al. 2023

JoF

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In the study of fungal pathogenesis, alternative methods have gained prominence due to recent global legislation restricting the use of mammalian animals in research. The principle of the 3 Rs (replacement, reduction, and refinement) is integrated into regulations and guidelines governing animal experimentation in nearly all countries. This principle advocates substituting vertebrate animals with other invertebrate organisms, embryos, microorganisms, or cell cultures. This review addresses host–fungus interactions by employing three-dimensional (3D) cultures, which offer more faithful replication of the in vivo environment, and by utilizing alternative animal models to replace traditional mammals. Among these alternative models, species like Caenorhabditis elegans and Danio rerio share approximately 75% of their genes with humans. Furthermore, models such as Galleria mellonella and Tenebrio molitor demonstrate similarities in their innate immune systems as well as anatomical and physiological barriers, resembling those found in mammalian organisms.

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Section: The Tenebrio Molitor Modelmentioning

confidence: 99%

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Fusco-Almeida,

de Matos Silva,

dos Santos

et al. 2023

JoF

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Extracellularly Released Molecules by the Multidrug-Resistant Fungal Pathogens Belonging to the Scedosporium Genus: An Overview Focused on Their Ecological Significance and Pathogenic Relevance

Mello

Barcellos

Aor

et al. 2022

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The multidrug-resistant species belonging to the Scedosporium genus are well recognized as saprophytic filamentous fungi found mainly in human impacted areas and that emerged as human pathogens in both immunocompetent and immunocompromised individuals. It is well recognized that some fungi are ubiquitous organisms that produce an enormous amount of extracellular molecules, including enzymes and secondary metabolites, as part of their basic physiology in order to satisfy their several biological processes. In this context, the molecules secreted by Scedosporium species are key weapons for successful colonization, nutrition and maintenance in both host and environmental sites. These biologically active released molecules have central relevance on fungal survival when colonizing ecological places contaminated with hydrocarbons, as well as during human infection, particularly contributing to the invasion/evasion of host cells and tissues, besides escaping from the cellular and humoral host immune responses. Based on these relevant premises, the present review compiled the published data reporting the main secreted molecules by Scedosporium species, which operate important physiopathological events associated with pathogenesis, diagnosis, antimicrobial activity and bioremediation of polluted environments.

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Assessing Differences between Clinical Isolates of Aspergillus fumigatus from Cases of Proven Invasive Aspergillosis and Colonizing Isolates with Respect to Phenotype (Virulence in Tenebrio molitor Larvae) and Genotype

Cited by 2 publications

References 76 publications

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Extracellularly Released Molecules by the Multidrug-Resistant Fungal Pathogens Belonging to the Scedosporium Genus: An Overview Focused on Their Ecological Significance and Pathogenic Relevance

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