Assessing different liquid-storage temperatures for rooster spermatozoa

Blank, Marcel Henrique; Ruivo, Lourenço Proença; Novaes, Gabriel A.; Lemos, Evelyn C.; Losano, João Diego de Agostini; Siqueira, Adriano Felipe Perez; Pereira, Ricardo José Garcia

doi:10.1016/j.anireprosci.2021.106845

Cited by 8 publications

(8 citation statements)

References 48 publications

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“…Spermatozoa motility in semen typically decreases over the storage period, aligning with findings by Blank et al (2021), which note a significant decline in spermatozoa motility poststorage. Semen storage induces changes in spermatozoa quality due to increased oxidative stress, which in turn diminishes motility (Víquez et al, 2020).…”

Section: Spermatozoa Motilitysupporting

confidence: 78%

Supplementation of Glycine and Glucose into Egg Yolk Lactated Ringer Diluent on The Quality of Local Chicken Semen Stored at 5oC for 120 Hours

Junaedi,

Isnaini,

Natsir

et al. 2024

J. Med. Vet.

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The impact of supplementing glucose, glycine, or a combination of both in Ringer’s lactate egg yolk base extender to preserve the quality of semen from local Indonesian chickens has not been previously investigated. This study aimed to examine the potential of glucose and glycine on chicken semen stored at 5°C for 120 hours. In this study, five local roosters were used. The parameters under observation included semen volume, odor, pH levels, consistency, color, mass movement, concentration, motility, viability, abnormality, plasma membrane integrity, chromatin degeneration, and acrosomal cap integrity. This study was conducted using a completely randomized design (CRD) with four treatments groups and 10 replication, i.e. T1 (control without supplementation), T2 (50 mM glucose), T3 (60 mM glycine), and T4 (a combination of 50 mM glucose and 60 mM glycine), respectively. In result, semen volume was 0.54 ± 0.17 mL/ejaculate, a milky white color, distinctive odor, thick consistency, good mass movement (++/+++), pH of 7.37 ± 0.23, motility of 91.50 ± 2.42%, plasma membrane integrity of 96.85 ± 0.96%, abnormality at 2.88 ± 0.77%, the concentration of 3.04 ± 0.3 billion/mL, and viability of 96.47 ± 1.71%. Following storage at 5°C for 120 hours, the motility, viability, abnormality, and acrosomal cap integrity of local chicken spermatozoa significantly different (p < 0.05) between T3 and T4 compared to T1 and T2 groups. Moreover, the integrity of the plasma membrane and chromatin degeneration in treatment T3 significantly different (p < 0.05) from T1, T2, and T4 groups. In conclusion, local chickens exhibited fair quality fresh semen both in macroscopic and microscopic evaluations. Furthermore, the combination of 60 mM glycine and 50 mM glucose into local chicken semen stored at 5°C for 120 hours effectively preserved motility and viability, minimized abnormality, maintained plasma membrane integrity, minimized chromatin degeneration, and retained acrosomal integrity.

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Section: Spermatozoa Motilitysupporting

confidence: 78%

Supplementation of Glycine and Glucose into Egg Yolk Lactated Ringer Diluent on The Quality of Local Chicken Semen Stored at 5oC for 120 Hours

Junaedi,

Isnaini,

Natsir

et al. 2024

J. Med. Vet.

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“…Sperm DNA integrity is an important variable associated with fertility and early embryonic development ( Hamilton and Assumpção, 2020 ) and can be evaluated in roosters by comet assay ( Gliozzi et al, 2011 ), sperm chromatin structure assay ( Salehi et al, 2020 ; Blank et al, 2021 ), and sperm chromatin dispersion ( SCD ) ( Shanmugam et al, 2014 ; Johnston et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

L-arginine supplementation minimizes aging-induced changes in the sperm chromatin of roosters

Oliveira

Butolo

Butolo³

et al. 2023

Poultry Science

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“…The high temperature of the Makler chamber reactivates cell activity causing a sperm motility recovery. To preserve fertilization capacity in poultry, pooled semen is normally stored at 2–8°C so that sperm metabolism decreases and less reactive oxygen species (ROS) are produced [ 46 – 48 ]. In the stallion, a temperature about 5°C has been determined as the optimal storage temperature for maintenance of motility and fertility [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

Madeddu

Marelli

Sayed

et al. 2022

Veterinary Medicine International

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In order to preserve endangered psittacine species, more basic and applied research in reproductive biology is required. Assisted reproductive technologies such as artificial insemination play an important role in parrots species conservation programs to overcome the problem of infertile eggs and male infertility. The aim of this study was to define an effective in vitro protocol in order to standardize the sperm quality evaluation in psittacines, studying Melopsittacus undulatus as model species. Semen was collected from twenty adult males by massage technique from May to June. Sperm concentration was measured by the spectrophotometric method. Sperm quality (sperm membrane integrity (SMI), motility, and kinetic parameters) was assessed on fresh semen. Three different experimental protocols were performed to compare the effects of various processing conditions on SMI, motility, and kinetic parameters. In protocol 1, test was performed by Lake extender with three different pH, 7.4 versus 8.2 versus 8.4, and two different equilibration temperatures after dilution of fresh semen (4°C versus 25°C). In protocol 2, two dilution rates of semen after collection were valuated, 1 : 3 versus 1 : 4, as well as three different semen storage temperatures (4°C versus 25°C versus 38°C) before sperm motility analysis with the computer-assisted sperm analysis (CASA). In protocol 3, two different Makler chamber temperatures (38 versus 41°C) during motility analysis were tested. A significant progressive improvement in spermatozoa motility and kinetic parameters was registered with pH 8.4. Progressive motility and all kinetic parameters were higher at 4°C equilibration temperature. Straightness (STR) kinetic parameter was better with 1 : 4 dilution rate. Total motile sperm was higher in 41°C Makler chamber. In this study, for the first time, the effects of different processing protocols on psittacines seminal quality analysis were investigated. Significant differences conditioning the effectiveness of analysis protocols have been described.

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Assessing different liquid-storage temperatures for rooster spermatozoa

Cited by 8 publications

References 48 publications

Supplementation of Glycine and Glucose into Egg Yolk Lactated Ringer Diluent on The Quality of Local Chicken Semen Stored at 5oC for 120 Hours

Supplementation of Glycine and Glucose into Egg Yolk Lactated Ringer Diluent on The Quality of Local Chicken Semen Stored at 5oC for 120 Hours

L-arginine supplementation minimizes aging-induced changes in the sperm chromatin of roosters

Assessment of Sperm Viability and Computer-Assisted Motility Analysis in Budgerigars (Melopsittacus undulatus): Effect of Several In Vitro Processing Conditions

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