Context. Measurement and improvement of informal caregiver burden are central aims of policy and intervention. Burden itself is a complex construct, and total burden can differ by patient diagnosis, although how diagnosis affects different aspects of caregiver subjective burden is unclear.Objectives. To compare the subjective burden of caregivers across three diagnostic groups using the 22-item Zarit Burden Inventory.Methods. We performed a secondary analysis of pooled cross-sectional data from four U.K. studies of informal caregivers of patients with advanced cancer (n ÂŒ 105), dementia (n ÂŒ 131), and acquired brain injury (ABI) (n ÂŒ 215). Zarit Burden Inventory totals, subscales (personal and role strain), and individual mean scores were compared between diagnostic groups using the general linear model, adjusting for caregiver characteristics. Conclusion. Our data show that total, subscale, and most individual elements of caregiver subjective burden differ between cancer, dementia, and ABI caregivers. This should be considered when designing future intervention strategies to reduce caregiver burden in these groups. J Pain Symptom Manage 2015;-:-e-. Ă