Assessing Genetic Diversity in Olea europaea L. Using ISSR and SSR Markers

Gomes, Sónia; Martins-Lopes, Paula; Lopes, João M.; Guedes-Pinto, Henrique

doi:10.1007/s11105-009-0106-3

Cited by 48 publications

(43 citation statements)

References 27 publications

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“…The high level of polymorphism at ISSR loci also indicates high genetic variability in wild olive trees, which is in agreement with other studies made on cultivated (Gemas et al, 2004;Terzopoulos et al, 2005;Essadki et al, 2006;Gomes et al, 2009;Martins-Lopes et al, 2009) and wild olive trees (Hess et al, 2000).…”

Section: Discussionsupporting

confidence: 92%

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Noormohammadi

Samadi-Molayousefi²,

Sheidai³

2012

Genet. Mol. Res.

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ABSTRACT. Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR markers. The mean value of polymorphism for RAPD loci was 73.71%, while the value for ISSR loci was 81.74%. The Keshar population had the highest value of intra-population polymorphism for both RAPD and ISSR loci (66.86 and 62.71%, respectively), while the Tudar population had the lowest values (20.35 and 28.81%, respectively). Similarly, the highest and lowest number of effective alleles, Shannon index and Nei's genetic diversity were also found for these two populations. The highest value of H pop /H sp within population genetic diversity for RAPD and ISSR loci was found for the Keshar population (H pop = 0.85 and H sp = 0.90). OPA04-750, OPA13-650 and OPA02-350 RAPD bands were specific for Tudar, Bondon and Keshar populations, respectively, while no specific ISSR bands were observed. Analysis of molecular variance as well as the pairwise F ST test showed significant differences for RAPD and ISSR markers among the populations. The NJ and UPGMA trees also separated the wild olive populations from each other, indicating their genetic distinctness. UPGMA clustering of the four wild olive populations placed the Tudar population far from the other populations; Keshar and Bokhoon population samples revealed more similarity and were grouped together. We conclude that there is high genetic diversity among O. europaea ssp cuspidata populations located in southern Iran. We also found RAPD and ISSR markers to be useful molecular tools to discriminate and evaluate genetic variations in wild olive trees.

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Section: Discussionsupporting

confidence: 92%

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Noormohammadi

Samadi-Molayousefi²,

Sheidai³

2012

Genet. Mol. Res.

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“…In the present study, the efficiency of both ISSR and SCoT markers is estimated through parameters such as PIC, MI and Rp. Parameters such as PIC have been used usually for evaluating the informative potential of ISSR markers in different germplasm and cultivated genotypes [24][25][26][27]. Based on the results of data analysis, the moderate values of PIC for both marker primers could be attributed to evaluation of genetic diversity.…”

Section: B D (Non A) D D (Non C) Y D (C T) and R D (A G)mentioning

confidence: 99%

Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes

Etminan

Pour-Aboughadareh

Mohammadi

et al. 2016

Biotechnology & Biotechnological Equipment

124

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Durum wheat (Triticum turgidum var. durum) is one of the most important cereal crops widely cultivated all over the world with high economic value. In the present study, genetic variation in a mini-core collection of durum wheat germplasm, including 25 breeding lines and 18 landraces, was evaluated using 15 inter-simple sequence repeat (ISSR) and six start codon targeted (SCoT) markers. High levels of polymorphism were observed; 98.70% (ISSR) and 100% (SCoT), which indicated that these markers are useful tools for detection of genetic variation in the collection. Analysis of molecular variance revealed that the major part of genetic variations (90% and 93% for ISSR and SCoT, respectively) occurred within genotypes set. Comparing the genetic variation of breeding lines and landraces based on genetic parameters showed that effective number of alleles (Ne), Nei's gene diversity (He) and Shannon's Information index (I) in landraces were higher than in breeding lines. Although cluster analysis, based on both markers, separated the genotypes in five groups, the dendrogram obtained from SCoT provided the best clustering pattern. Inter-population differentiation (Gst) estimated on the basis of two marker systems representing that a vast portion of the total genetic diversity refers to variation within two sets of genotypes. In conclusion, the results verified a high level of genetic variation among the durum wheat mini-core collection, particularly among landraces, which can be interesting for future breeding programmes.

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“…Nesse sentido, alguns estudos sobre a diversidade genética de cultivares de oliveira revelam a importância do conhecimento da origem e do efeito da região agroecológica de cultivo (Gemas et al, 2004), e outros confirmam a semelhança genética entre as cultivares (Martins-Lopes et al, 2007;Cordeiro et al, 2008), a existência de clones entre elas (Rony et al, 2009) e a necessidade de garantir a diferença entre as cultivares para fins de certificação (Gomes et al, 2009 …”

Section: Análise De Correlaçõesunclassified

Avaliação da interação micorrízica em cultivares de oliveira (Olea europea L.)

Vieira¹,

Melloni²,

Neto³

2011

Rev. Bras. Ciênc. Solo

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A maioria dos estudos com oliveira no Brasil e no mundo tem sido direcionada à produção e ao manejo. Apesar da importância para o crescimento vegetal e resistência a estresses diversos, não há estudos sobre a ocorrência de associação micorrízica em oliveira, no Brasil. O objetivo do presente trabalho foi avaliar a interação micorrízica em cultivares de oliveira na Fazenda Experimental de Maria da Fé - MG (FEMF - EPAMIG), a qual serve como ponto de partida na produção de inoculantes comerciais de fungos micorrízicos arbusculares para a cultura. Amostras de solo e de raízes de sete cultivares de oliveira foram coletadas para determinação do comprimento de micélio extrarradicular ativo e total e avaliação da percentagem e intensidade de colonização micorrízica, bem como da densidade e diversidade de esporos. Nas rizosferas das cultivares de oliveira, foram isoladas nove espécies de fungos micorrízicos: Acaulospora denticulata, Acaulospora scrobiculata, Acaulospora sp1, Acaulospora sp2, Entrophospora sp1, Gigaspora sp1, Glomus mosseae, Scutellospora pellucida e Scutellospora sp1. Os resultados comprovaram o micotrofismo da espécie. No entanto, não houve efeito das cultivares na distribuição dos propágulos de FMAs, os quais estiveram homogeneamente distribuídos nas diferentes rizosferas.

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Assessing Genetic Diversity in Olea europaea L. Using ISSR and SSR Markers

Cited by 48 publications

References 27 publications

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Intra-specific genetic diversity in wild olives (Olea europaea ssp cuspidata) in Hormozgan Province, Iran

Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes

Avaliação da interação micorrízica em cultivares de oliveira (Olea europea L.)

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