Assessing genetic relationships among bitter gourd (<i>Momordica charantia</i>L.) accessions using inter-simple sequence repeat (ISSR) markers

Singh, Ashutosh; Behera, T. K.; Chandel, D.; Sharma, Priya; Singh, Nisha

doi:10.1080/14620316.2007.11512222

Cited by 39 publications

(41 citation statements)

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“…5 The relative efficiency of marker types for genetic analysis varies among crop species. Russell et al 9 and ISSR (6.30) 10 analyses was dramatically lower. Garcia-Mas et al 19 observed similar differences in polymorphism when comparing AFLP (23.1%), RFLP (62%) and RAPD (18%) markers in melon (Cucumis melo L.).…”

Section: Resultsmentioning

confidence: 91%

Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.)

et al. 2008

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BACKGROUND: Bitter gourd (Momordica charantia L.) is an important vegetable crop in tropical countries, including China and India. A wide range of genetic diversity exists in India with respect to fruit morphology such as colour, size and exocarp. A diversity assessment conducted using different DNA marker systems amplified fragment length polymorphism (AFLP), inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers) will be helpful in the establishment of a broad-based description for improved germplasm curation and the identification of germplasm for genome mapping and breeding of bitter gourd.

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Section: Resultsmentioning

confidence: 91%

Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.)

et al. 2008

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“…Based on both historical literature [1,2,5], and recent analysis of random amplified polymorphic DNA [6], inter simple sequence repeats [7] and amplified fragment length polymorphisms [8] molecular analyses, eastern India (including the states of Orissa, West Bengal, Assam, Jharkhand and Bihar) may be considered as the primary center of the diversity of bitter gourd, where a wild feral form, M. charantia varieties also exists. M. dioica and M. charantia are ubiquitous in distribution over India except in north-east region, whereas M. subangulata ssp.…”

Section: Biogeography and Botanical Descriptionmentioning

confidence: 99%

Food prospects and nutraceutical attributes of Momordica species: A potential tropical bioresources – A review

Nagarani

Abirami

Siddhuraju

2014

Food Science and Human Wellness

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Plants with potential therapeutic values have been used from time immemorial to cure various ailments and infectious diseases. Of late, scientific evidences have been provided on the potential therapeutic agent exhibited by certain traditionally used vegetable extracts. The importance of wild edible plants may be traced to antiquity but systemic studies are recent. All the Momordica species have been consumed as vegetable and traditionally used for various disorders. The whole plant parts are ascribed to possess the anti-diabetic effect in traditional medicinal system. The active constituents of Momordica plant parts were cucurbitane type triterpenoids, phenolics, glycosides, and several kinds of peptides including Momordica anti-HIV protein (MAP 30). Recent reports revealed the presence of several kinds of cucurbitane type triterpenoids in leaf, stem and fruits of Momordica species having several pharmacological activities. There is lack of scientific information available on the wild species which also having several bioactive components with potential activities. So the present review compares and highlights the current knowledge of the nutritional value, phytochemistry and physiological effects of wild species with known variety.

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“…Random amplification of polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSRs) have been used to identify genetic variations between plant species because their applications does not require prior information regarding the target sequence in the genome (Khanuja et al, 1998) and require very little starting DNA template (Zietkiewicz et al, 1994). Thus, this technology has been widely used to investigate the genetic diversity and genetic structure of plant species (Singh et al, 2007;Liu et al, 2008;Ding et al, 2009;Ercisli et al, 2009). There have been no reports examining the genetic diversity of Gypsophila species.…”

Section: Introductionmentioning

confidence: 99%

Biogeographic pattern of genetic diversity detected by RAPD and ISSR analysis in Gypsophila (Caryophyllaceae) species from Turkey

Korkmaz

2015

Genet. Mol. Res.

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ABSTRACT. Gypsophila L. is the 3rd-largest genus of Caryophyllaceae in Turkey, a country that includes 60 taxa belonging to 56 species of the 126 recognized in the genus. A total of 35 taxa are endemic to Turkey, with an approximately 60% endemism ratio. In this study, the genetic diversity of 14 Gypsophila species from Turkey was analyzed using random amplification of polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Sixteen RAPD and 6 ISSR primers produced 132 polymorphic bands. RAPD, ISSR, and RAPD + ISSR primers for the 14 species showed 92.7, 93.8, and 92.9% polymorphism, respectively. Our results indicate that RAPD and ISSR markers are reliable and effective for assessing the genetic diversity of Gypsophila species. Gypsophila species studied were separated into 2 clusters. Our analysis shows that the clusters correlated with geographic and phytogeographic regions.

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Assessing genetic relationships among bitter gourd (Momordica charantiaL.) accessions using inter-simple sequence repeat (ISSR) markers

Cited by 39 publications

References 0 publications

Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.)

Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.)

Food prospects and nutraceutical attributes of Momordica species: A potential tropical bioresources – A review

Biogeographic pattern of genetic diversity detected by RAPD and ISSR analysis in Gypsophila (Caryophyllaceae) species from Turkey

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