Assessing heterogeneity of the high-mannose glycopeptide gp432 on the variant surface glycoprotein of trypanosomes: a comparison of plasma desorption mass spectrometry and radiolabeling techniques

Californium-252 plasma desorption mass spectrometry of oligosaccharides and glycoconjugates: control of ionization and fragmentation

“…Cotter et al have used PDMS to assess the heterogeneity of an underivatized high-mannose glycopeptide GP 432 from the variant surface glycoprotein of trypanosomes (32) and to assist in the elucidation of the structures of the lipid A portion of the lipopolysaccharides from the rough (Re) mutant of E. coli (33).…”

Analysis of glycoconjugates by fast atom bombardment mass spectrometry and related ms techniques

“…(+) and (-) plasma desorption (PD)-MS analysis of glycopeptides was shown to be suitable for determination of molecular size and degree of glycosylation estimation (143)(144)(145). Different MALD-and ES-MS techniques were used in similar way for assessment of N-and/or 0-glycosylation sites of T-lymphocyte glycoprotein receptor (146), human serotransferrin (147), recombinant human growth hormone and tissue plasminogen activator (148), hirudin from the leech Hirudinaria manillensis (149).…”

Time-of-flight mass spectrometry for the structural analysis of biological molecules

“…The relatively simple, straightforward molecular weight measurements obtained with TOF instruments can provide critical information about the final protein product. In our laboratory PD and/or MALD molecular weight measurements have been used to characterize the N-linked glycopeptides in bovine fetuin (27), to assess carbohydrate heterogeneity in trypanosomes (28) and phosphate heterogeneity in the riboflavin binding peptide (29), to determine cleavage sites in the processing of the amyloid precursor protein (30) and the assembly protein from simian cytomegalovirus (31), and to verify the structures of Staphylococcus nuclease insertion products (32).…”