The authors analyze phytoplankton diversity of a small urban wetland of Meghalaya to assess biodiversity and limnology interest of small water bodies. This “slightly acidic-circumneutral, demineralized and soft water” subtropical wetland reveals diverse phytoplankton (64 species), indicates high desmid richness and highlights the speciose littoral constellations of up to 55-58 species per sample. Phytoplankton comprises dominant quantitative component of net plankton and registers Charophyta dominance; Chlorophyta > Bacillariophyta > Dinozoa > Chrysophyta > Cyanobacteria depict sub-dominance, and Euglenozoa and Cryptophyta show poor abundance at the littoral and semi-limnetic regions. The richness of phytoplankton and abundance of phytoplankton, Charophyta, Chlorophyta, Dinozoa, Chrysophyta and Cyanobacteria follow bimodal spatio-temporal variations. Closterium, Cosmarium, Staurastrum, Micrasterias, Netrium, Staurodesmus and Scenedesmus are notable genera, and 14 species collectively influence phytoplankton abundance. Phytoplankton registers high species diversity, lower dominance and high evenness. Amongst 15 abiotic factors, only the rainfall and sulphate exert notable influence individually, while the canonical correspondence analysis registers lower cumulative influence of the selected 10 factors on the littoral and semi-limnetic phytoplankton assemblages. This study merits interest for neglected biodiversity and ecology of small aquatic biotopes of India and urban wetlands in particular.